A preliminary study in the use of RAPD markers in detecting genetic differences in hatching patterns of Chirocephalus diaphanus Prévost, ... View Full Text


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Article Info

DATE

2002-10

AUTHORS

P. Zarattini, V. Rossi, B. Mantovani, G. Mura

ABSTRACT

Randomly amplified polymorphic DNA (RAPD) was used to detect intra-clutch genetic differences of the anostracan Chirocephalus diaphanus Prévost, 1803. Thirty primers were tested on 130 nauplii from three different clutches. Two primers produced five repeatable and well-defined amplification products, that were used as genotype markers. Nauplii hatched after three successive dehydration and rehydration cycles. Consistent genetic differentiation was observed among nauplii from the same clutch. Significant association between presence or absence of three polymorphic amplification products and hatching after different stimuli was observed. These results suggest that RAPD markers might be related to the difference in nauplii hatching and provide reasonable support to the hypothesis that the source of the observed variation in hatching phenology are genetic as well as epigenetic. More... »

PAGES

315-323

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1023/a:1021335826251

    DOI

    http://dx.doi.org/10.1023/a:1021335826251

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1024121437


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