Genetic variability evaluation in a Moroccan collection of barley, Hordeum vulgare L., by means of storage proteins and RAPDs View Full Text


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Article Info

DATE

2002-12

AUTHORS

EL-Habib Dakir, María-Luisa Ruiz, Pedro García, Marcelino Pérez de la Vega

ABSTRACT

The genetic variation existing in a set of barley (Hordeum vulgare L.) landrace samples recently collected in Morocco was estimated. Two kinds of genetic markers, seed storage proteins (hordeins) and random amplified polymorphic DNA (RAPD), were used. Only six out of 31 landraces were subjected to RAPD analysis. Both kinds of markers, RAPD and storage proteins, yielded similar results, showing that the level of variation observed in Moroccan barley was high: all landraces showed variability; 808 different storage protein patterns (multilocus associations) were observed among 1897 individuals (2.32 seeds per association, on average) with an average of 43 multilocus associations per accession. In general, genetic variation within accessions was higher than between accessions. The 100 polymorphic RAPD bands generated by 21 effective primers were able to generate enough patterns to differentiate between uniform cultivars and even between individuals in variable accessions. One of the aims of this work was to compare the effectiveness of RAPD versus storage protein techniques in assessing the variability of genetic resource collections. On average hordeins were more polymorphic than RAPDs: they showed more alternatives per band on gels and a higher percentage of polymorphic bands, although RAPDs supply a higher number of bands. Although RAPD is an easy and standard technique, storage protein analysis is technically easier, cheaper and needs less sophisticated equipment. Thus, when resources are a limiting factor and considering the cost of consumables and work time, seed storage proteins must be the technique of choice for a first estimation of genetic variation in plant genetic resource collections. More... »

PAGES

619-631

References to SciGraph publications

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  • 2000-04. Genetic diversity of barley landrace accessions (Hordeum vulgare ssp. vulgare) conserved for different lengths of time in ex situ gene banks in HEREDITY
  • 1999-06. RAPD divergence caused by microsite edaphic selection in wild barley in GENETICA
  • 1993-02. Random amplified polymorphic DNA and pedigree relationships in spring barley in THEORETICAL AND APPLIED GENETICS
  • 1987-10. Wheat storage proteins: diversity of HMW glutenin subunits in wild emmer from Israel in THEORETICAL AND APPLIED GENETICS
  • 1986-11. Proposal for the identification of barley varieties based on the genotypes for 2 hordein and 39 isoenzyme loci of 47 reference varieties in EUPHYTICA
  • 1993-01. Genetic constitution for spring growth habit and some other characters in barley cultivars in the Mediterranean coastal regions in EUPHYTICA
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  • 1983-09. A plant DNA minipreparation: Version II in PLANT MOLECULAR BIOLOGY REPORTER
  • 2000-01. RAPD characterization of Brazilian barley (Hordeum vulgare ssp. vulgare) varieties in EUPHYTICA
  • 1986-09. Genetic diversity and environmental associations of wild barley,Hordeum spontaneum (Poaceae), in Iran in PLANT SYSTEMATICS AND EVOLUTION
  • 1999-06. Malting enzyme activities, grain protein variation and yield potentials in the displaced genetic resources of barley landraces of Finland in GENETIC RESOURCES AND CROP EVOLUTION
  • 1997. Plant genetic adaptedness to climatic and edaphic environment in ADAPTATION IN PLANT BREEDING
  • 1997-06. The assessment of genetic variation in Spanish primitive cultivars of barley, Hordeum vulgare L., by a combination of isozymes and hordeins in GENETIC RESOURCES AND CROP EVOLUTION
  • 2001-02. RAPD data do not support a second centre of barley domestication in Morocco in GENETIC RESOURCES AND CROP EVOLUTION
  • 1994-04. Ecogeographical distribution and differential adaptedness of multilocus allelic associations in Spanish Avena sativa L. in THEORETICAL AND APPLIED GENETICS
  • 1999-11. Genetic diversity of old French six-rowed winter barley varieties assessed with molecular, biochemical and morphological markers and its relation to BaMMV resistance in HEREDITY
  • 1983-06. A differential rate of regional distribution of barley flavonoid patterns in Ethiopia, and a view on the center of origin of barley in HEREDITAS
  • 1970-08. Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 in NATURE
  • Journal

    TITLE

    Genetic Resources and Crop Evolution

    ISSUE

    6

    VOLUME

    49

    Author Affiliations

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1023/a:1021228730714

    DOI

    http://dx.doi.org/10.1023/a:1021228730714

    DIMENSIONS

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    38 schema:description The genetic variation existing in a set of barley (Hordeum vulgare L.) landrace samples recently collected in Morocco was estimated. Two kinds of genetic markers, seed storage proteins (hordeins) and random amplified polymorphic DNA (RAPD), were used. Only six out of 31 landraces were subjected to RAPD analysis. Both kinds of markers, RAPD and storage proteins, yielded similar results, showing that the level of variation observed in Moroccan barley was high: all landraces showed variability; 808 different storage protein patterns (multilocus associations) were observed among 1897 individuals (2.32 seeds per association, on average) with an average of 43 multilocus associations per accession. In general, genetic variation within accessions was higher than between accessions. The 100 polymorphic RAPD bands generated by 21 effective primers were able to generate enough patterns to differentiate between uniform cultivars and even between individuals in variable accessions. One of the aims of this work was to compare the effectiveness of RAPD versus storage protein techniques in assessing the variability of genetic resource collections. On average hordeins were more polymorphic than RAPDs: they showed more alternatives per band on gels and a higher percentage of polymorphic bands, although RAPDs supply a higher number of bands. Although RAPD is an easy and standard technique, storage protein analysis is technically easier, cheaper and needs less sophisticated equipment. Thus, when resources are a limiting factor and considering the cost of consumables and work time, seed storage proteins must be the technique of choice for a first estimation of genetic variation in plant genetic resource collections.
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