Characterization of transgenic rice plants over-expressing the stress-inducible β-glucanase gene Gns1 View Full Text


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Article Info

DATE

2003-01

AUTHORS

Yoko Nishizawa, Masayasu Saruta, Kyoko Nakazono, Zenta Nishio, Masato Soma, Takanobu Yoshida, Emi Nakajima, Tadaaki Hibi

ABSTRACT

The Gns1 gene of rice (Oryza sativa L. japonica) encodes 1,3;1,4-β glucanase (EC 3.2.1.73), which hydrolyzes 1,3;1,4-β-glucosidic linkages on 1,3;1,4-β-glucan, an important component of cell walls in the Poaceae family. RNA and protein gel blot analyses demonstrated that blast disease or dark treatment induced the expression of the Gns1 gene. To assess the function of the Gns1 gene in disease resistance, we characterized transgenic rice plants constitutively expressing the Gns1 gene. The introduced Gns1 gene was driven by the CaMV 35S promoter and its products were found in the apoplast and accumulated in up to 0.1% of total soluble protein in leaves. Although transgenic plants showed stunted growth and impaired root formation, fertility, germination, and coleoptile elongation appeared unaffected compared to non-transgenic control plants, indicating that Gns1 does not play a crucial role in rice germination and coleoptile elongation. When transgenic plants were inoculated with virulent blast fungus (Magnaporthe grisea), they developed many resistant-type lesions on the inoculated leaf accompanying earlier activation of defense-related genes PR-1 and PBZ1 than in control plants. Transgenic plants spontaneously produced brown specks, similar in appearance to those reported for an initiation type of disease-lesion-mimic mutants, on the third and fourth leaves and occasionally on older leaves without inoculation of pathogens. Expression of the two defense-related genes was drastically increased after the emergence of the lesion-mimic phenotype. More... »

PAGES

143-152

References to SciGraph publications

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  • 1999-08. Enhanced resistance to blast (Magnaporthe grisea) in transgenic Japonica rice by constitutive expression of rice chitinase in THEORETICAL AND APPLIED GENETICS
  • 1994-08-01. Enhanced Protection Against Fungal Attack by Constitutive Co–expression of Chitinase and Glucanase Genes in Transgenic Tobacco in NATURE BIOTECHNOLOGY
  • 1995-07-01. Genetic Engineering of Rice for Resistance to Sheath Blight in NATURE BIOTECHNOLOGY
  • 1990. Fungal Cell Walls — A Review in BIOCHEMISTRY OF CELL WALLS AND MEMBRANES IN FUNGI
  • 2001-09. Structure-function relationships of β- D-glucan endo- and exohydrolases from higher plants in PLANT MOLECULAR BIOLOGY
  • 2000-05. A Recessive Lesion Mimic Mutant of Rice with Elevated Resistance to Fungal Pathogens in JOURNAL OF GENERAL PLANT PATHOLOGY
  • 1992-07. Structure of the genes encoding Hordeum vulgare (1→3,1→4)-β-glucanase isoenzymes I and II and functional analysis of their promoters in barley aleurone protoplasts in MOLECULAR GENETICS AND GENOMICS
  • 1990-12. Structure and tissue-specific regulation of genes encoding barley (1→3, 1→4)-β-glucan endohydrolases in MOLECULAR GENETICS AND GENOMICS
  • 1997-09. Large-scale EST sequencing in rice in PLANT MOLECULAR BIOLOGY
  • 1999-03. Regulation of the chitinase gene expression in suspension-cultured rice cells by N-acetylchitooligosaccharides: differences in the signal transduction pathways leading to the activation of elicitor-responsive genes in PLANT MOLECULAR BIOLOGY
  • 1993-08. Purification and characterization of (1→3, 1→4)-β-glucan endohydrolases from germinated wheat (Triticum aestivum) in PLANT MOLECULAR BIOLOGY
  • 1992-01. Structure of a rice β-glucanase gene regulated by ethylene, cytokinin, wounding, salicylic acid and fungal elicitors in PLANT MOLECULAR BIOLOGY
  • 1989-04. Cauliflower mosaic virus gene VI causes growth suppression, development of necrotic spots and expression of defence-related genes in transgenic tobacco plants in MOLECULAR GENETICS AND GENOMICS
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    http://scigraph.springernature.com/pub.10.1023/a:1020714426540

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    https://www.ncbi.nlm.nih.gov/pubmed/12602898


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    33 schema:description The Gns1 gene of rice (Oryza sativa L. japonica) encodes 1,3;1,4-β glucanase (EC 3.2.1.73), which hydrolyzes 1,3;1,4-β-glucosidic linkages on 1,3;1,4-β-glucan, an important component of cell walls in the Poaceae family. RNA and protein gel blot analyses demonstrated that blast disease or dark treatment induced the expression of the Gns1 gene. To assess the function of the Gns1 gene in disease resistance, we characterized transgenic rice plants constitutively expressing the Gns1 gene. The introduced Gns1 gene was driven by the CaMV 35S promoter and its products were found in the apoplast and accumulated in up to 0.1% of total soluble protein in leaves. Although transgenic plants showed stunted growth and impaired root formation, fertility, germination, and coleoptile elongation appeared unaffected compared to non-transgenic control plants, indicating that Gns1 does not play a crucial role in rice germination and coleoptile elongation. When transgenic plants were inoculated with virulent blast fungus (Magnaporthe grisea), they developed many resistant-type lesions on the inoculated leaf accompanying earlier activation of defense-related genes PR-1 and PBZ1 than in control plants. Transgenic plants spontaneously produced brown specks, similar in appearance to those reported for an initiation type of disease-lesion-mimic mutants, on the third and fourth leaves and occasionally on older leaves without inoculation of pathogens. Expression of the two defense-related genes was drastically increased after the emergence of the lesion-mimic phenotype.
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    117 schema:name Characterization of transgenic rice plants over-expressing the stress-inducible β-glucanase gene Gns1
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