Ontology type: schema:ScholarlyArticle
2002-09
AUTHORSL. V. Basova, N. B. Ilyina, K. S. Vassilenko, E. I. Tiktopulo, V. E. Bychkova
ABSTRACTCytochrome b5 is a membrane protein that comprises two fragments: one is water-soluble and heme-containing, and the other is hydrophobic and membrane-embedded. The function of electron transfer is performed by the former whose crystal structure is known; however, its conformational states when in the membrane field and interacting with other proteins are still to be studied. Previously, we proposed water–alcohol mixtures for modeling the effect of membrane surface on proteins, and used this approach to study the conformational behavior of positively charged cytochrome c as well as relatively neutral retinol-binding protein also functioning in the field of a negatively charged membrane. The current study describes the conformational behavior of the negatively charged water-soluble fragment of cytochrome b5 as dependent on pH. Decreasing pH was shown to transform the fragment state from native to intermediate, similar to the molten globule reported earlier for other proteins in aqueous solutions: at pH 3.0, the fragment preserved a pronounced secondary structure and compactness but lost its rigid tertiary structure. A possible role of this intermediate state in cytochrome b5 functioning is discussed. More... »
PAGES718-725
http://scigraph.springernature.com/pub.10.1023/a:1020687800727
DOIhttp://dx.doi.org/10.1023/a:1020687800727
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1003577489
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biochemistry and Cell Biology",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Institute of Protein Research",
"id": "https://www.grid.ac/institutes/grid.418952.3",
"name": [
"Institute of Protein Research, Russian Academy of Sciences, 142290, Pushchino, Moscow Region, Russia"
],
"type": "Organization"
},
"familyName": "Basova",
"givenName": "L. V.",
"id": "sg:person.0652623571.68",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0652623571.68"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Institute of Protein Research",
"id": "https://www.grid.ac/institutes/grid.418952.3",
"name": [
"Institute of Protein Research, Russian Academy of Sciences, 142290, Pushchino, Moscow Region, Russia"
],
"type": "Organization"
},
"familyName": "Ilyina",
"givenName": "N. B.",
"id": "sg:person.015565450337.14",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015565450337.14"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Institute of Protein Research",
"id": "https://www.grid.ac/institutes/grid.418952.3",
"name": [
"Institute of Protein Research, Russian Academy of Sciences, 142290, Pushchino, Moscow Region, Russia"
],
"type": "Organization"
},
"familyName": "Vassilenko",
"givenName": "K. S.",
"id": "sg:person.01243765636.34",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01243765636.34"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Institute of Protein Research",
"id": "https://www.grid.ac/institutes/grid.418952.3",
"name": [
"Institute of Protein Research, Russian Academy of Sciences, 142290, Pushchino, Moscow Region, Russia"
],
"type": "Organization"
},
"familyName": "Tiktopulo",
"givenName": "E. I.",
"id": "sg:person.054365237.19",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.054365237.19"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Institute of Protein Research",
"id": "https://www.grid.ac/institutes/grid.418952.3",
"name": [
"Institute of Protein Research, Russian Academy of Sciences, 142290, Pushchino, Moscow Region, Russia"
],
"type": "Organization"
},
"familyName": "Bychkova",
"givenName": "V. E.",
"id": "sg:person.01063655771.30",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01063655771.30"
],
"type": "Person"
}
],
"citation": [
{
"id": "https://doi.org/10.1016/0014-5793(88)80223-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001898197"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0300-9084(94)90144-9",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1003900336"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0300-9084(94)90144-9",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1003900336"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0003-9861(71)90186-x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1005763884"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0079-6107(85)90004-5",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1008219389"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1006/bbrc.1998.8502",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1011722568"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/pro.5560020914",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1016952108"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0022-2836(72)90510-4",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018363084"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00248050",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1019794945",
"https://doi.org/10.1007/bf00248050"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00248050",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1019794945",
"https://doi.org/10.1007/bf00248050"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s1359-0278(98)00039-x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1021264499"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s1359-0278(98)00039-x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1021264499"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.77.4.1917",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022094288"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1074/jbc.271.44.27438",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1023336224"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.68.5.1042",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1024461485"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0022-2836(74)90188-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027426104"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1098/rstb.1995.0043",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1029033801"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1006/jmbi.1996.0241",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1034990547"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf02110101",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1040214012",
"https://doi.org/10.1007/bf02110101"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf02110101",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1040214012",
"https://doi.org/10.1007/bf02110101"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.71.11.4565",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1040847835"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0005-2795(80)90172-5",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044604983"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0005-2795(80)90172-5",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044604983"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1432-1033.1990.tb19281.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1047681751"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/bip.1981.360201102",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1047793856"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0014-5793(90)81180-v",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1052329522"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/bi00030a005",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055157893"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/bi00083a037",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055160170"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/bi00098a012",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055160809"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/bi00105a010",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055161064"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/bi00161a014",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055163225"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/bi00370a049",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055172499"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/bi00421a003",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055175160"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/bi00446a035",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055176568"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/bi00457a025",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055177027"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/bi00460a004",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055177132"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/bi00537a021",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055179923"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/bi00591a026",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055181934"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/bi9522460",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055211001"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/bi9522460",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055211001"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/bi961895o",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055212653"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/bi961895o",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055212653"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/bi9805036",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055215712"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/bi9805036",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055215712"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/bi980885f",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055215953"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/bi980885f",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055215953"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1126/science.2154849",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1062524618"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1126/science.2837825",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1062564697"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1075951686",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1077861782",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1079734004",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1080109986",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1080771107",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1081999260",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1082228481",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1082228481",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1082355374",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1082820664",
"type": "CreativeWork"
}
],
"datePublished": "2002-09",
"datePublishedReg": "2002-09-01",
"description": "Cytochrome b5 is a membrane protein that comprises two fragments: one is water-soluble and heme-containing, and the other is hydrophobic and membrane-embedded. The function of electron transfer is performed by the former whose crystal structure is known; however, its conformational states when in the membrane field and interacting with other proteins are still to be studied. Previously, we proposed water\u2013alcohol mixtures for modeling the effect of membrane surface on proteins, and used this approach to study the conformational behavior of positively charged cytochrome c as well as relatively neutral retinol-binding protein also functioning in the field of a negatively charged membrane. The current study describes the conformational behavior of the negatively charged water-soluble fragment of cytochrome b5 as dependent on pH. Decreasing pH was shown to transform the fragment state from native to intermediate, similar to the molten globule reported earlier for other proteins in aqueous solutions: at pH 3.0, the fragment preserved a pronounced secondary structure and compactness but lost its rigid tertiary structure. A possible role of this intermediate state in cytochrome b5 functioning is discussed.",
"genre": "research_article",
"id": "sg:pub.10.1023/a:1020687800727",
"inLanguage": [
"en"
],
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1356887",
"issn": [
"0026-8933",
"1608-3245"
],
"name": "Molecular Biology",
"type": "Periodical"
},
{
"issueNumber": "5",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "36"
}
],
"name": "Conformational States of the Water-Soluble Fragment of Cytochrome b5. I. pH-Induced Denaturation",
"pagination": "718-725",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"110ab787276b1c7e0eb3d3d7810b7f7a48da761af6f2d8888dfb83b0b4af960d"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1023/a:1020687800727"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1003577489"
]
}
],
"sameAs": [
"https://doi.org/10.1023/a:1020687800727",
"https://app.dimensions.ai/details/publication/pub.1003577489"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-11T01:12",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8697_00000536.jsonl",
"type": "ScholarlyArticle",
"url": "http://link.springer.com/10.1023%2FA%3A1020687800727"
}
]
Download the RDF metadata as:Â json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1023/a:1020687800727'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1023/a:1020687800727'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1023/a:1020687800727'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1023/a:1020687800727'
This table displays all metadata directly associated to this object as RDF triples.
226 TRIPLES
21 PREDICATES
75 URIs
19 LITERALS
7 BLANK NODES