Preparative High-Resolution Two-Dimensional Electrophoresis Enables the Identification of RNA Polymerase B Transcription Factor 3 as an Apoptosis-Associated Protein in the ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1999-02

AUTHORS

Ekkehard Brockstedt, Albrecht Otto, Anke Rickers, Kurt Bommert, Brigitte Wittmann-Liebold

ABSTRACT

Apoptosis or programmed cell death is essential in the process of controlling lymphocyte growth and selection. We identified RNA polymerase B transcription factor 3 (BTF3), which is associated with anti-IgM antibody-mediated apoptosis, using a subclone of the human Burkitt lymphoma cell line BL60. To identify the transcription factor BTF3, which is expressed only in minor amounts, we used preparative high-resolution two-dimensional gel electrophoresis (2DE) employing carrier ampholytes for isoelectric focusing. Comparison of the 2DE protein patterns from apoptotic and nonapoptotic cells showed BTF3 as a predominantly altered protein spot. The characterization of the differentially expressed transcription factor and 13 marker proteins described in this study were performed by internal Edman microsequencing and/or by peptide mass fingerprinting using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). The proteome analysis was significantly improved by performing the newly developed preparative high-resolution two-dimensional gels employing high protein concentrations. More... »

PAGES

225-231

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1020636308270

DOI

http://dx.doi.org/10.1023/a:1020636308270

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1045264561

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/10333297


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