Ontology type: schema:ScholarlyArticle
2002-09
AUTHORSM. Beccerra, S. Díaz Prado, E. Rodríguez-Belmonte, M.E. Cerdán, M.I. González Siso
ABSTRACTA recombinant strain of Saccharomyces cerevisiae, secreting β-galactosidase from Kluyveromyces lactis, grew efficiently with more than 60 g lactose l−1. The growth rate (0.23 h−1) in a cheese-whey medium was close to the highest reported hitherto for other recombinant S. cerevisiae strains that express intracellular β-galactosidase and lactose-permease genes. The conditions for growth and β-galactosidase secretion in this medium were optimized in a series of factorial experiments. Best results were obtained at 23 °C for 72 h. Since the recombinant strain produced less than 3% ethanol from the lactose, it was also assayed for the production of fructose 1,6-bisphosphate from cheese whey, and 0.06 g l−1 h−1 were obtained. More... »
PAGES1391-1396
http://scigraph.springernature.com/pub.10.1023/a:1019886112076
DOIhttp://dx.doi.org/10.1023/a:1019886112076
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