Metabolic engineering for direct lactose utilization by Saccharomyces cerevisiae View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2002-09

AUTHORS

M. Beccerra, S. Díaz Prado, E. Rodríguez-Belmonte, M.E. Cerdán, M.I. González Siso

ABSTRACT

A recombinant strain of Saccharomyces cerevisiae, secreting β-galactosidase from Kluyveromyces lactis, grew efficiently with more than 60 g lactose l−1. The growth rate (0.23 h−1) in a cheese-whey medium was close to the highest reported hitherto for other recombinant S. cerevisiae strains that express intracellular β-galactosidase and lactose-permease genes. The conditions for growth and β-galactosidase secretion in this medium were optimized in a series of factorial experiments. Best results were obtained at 23 °C for 72 h. Since the recombinant strain produced less than 3% ethanol from the lactose, it was also assayed for the production of fructose 1,6-bisphosphate from cheese whey, and 0.06 g l−1 h−1 were obtained. More... »

PAGES

1391-1396

Journal

TITLE

Biotechnology Letters

ISSUE

17

VOLUME

24

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1019886112076

DOI

http://dx.doi.org/10.1023/a:1019886112076

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1033617873


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1103", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Clinical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of A Coru\u00f1a", 
          "id": "https://www.grid.ac/institutes/grid.8073.c", 
          "name": [
            "Departamento de Biolox\u00eda Celular e Molecular, Facultade de Ciencias, Universidade da Coru\u00f1a, Campus da Zapateira s/n, 15071, A Coru\u00f1a, pSpain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Beccerra", 
        "givenName": "M.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of A Coru\u00f1a", 
          "id": "https://www.grid.ac/institutes/grid.8073.c", 
          "name": [
            "Departamento de Biolox\u00eda Celular e Molecular, Facultade de Ciencias, Universidade da Coru\u00f1a, Campus da Zapateira s/n, 15071, A Coru\u00f1a, pSpain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "D\u00edaz Prado", 
        "givenName": "S.", 
        "id": "sg:person.015730575154.33", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015730575154.33"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of A Coru\u00f1a", 
          "id": "https://www.grid.ac/institutes/grid.8073.c", 
          "name": [
            "Departamento de Biolox\u00eda Celular e Molecular, Facultade de Ciencias, Universidade da Coru\u00f1a, Campus da Zapateira s/n, 15071, A Coru\u00f1a, pSpain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rodr\u00edguez-Belmonte", 
        "givenName": "E.", 
        "id": "sg:person.01312110053.66", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01312110053.66"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of A Coru\u00f1a", 
          "id": "https://www.grid.ac/institutes/grid.8073.c", 
          "name": [
            "Departamento de Biolox\u00eda Celular e Molecular, Facultade de Ciencias, Universidade da Coru\u00f1a, Campus da Zapateira s/n, 15071, A Coru\u00f1a, pSpain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cerd\u00e1n", 
        "givenName": "M.E.", 
        "id": "sg:person.01302030267.03", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01302030267.03"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of A Coru\u00f1a", 
          "id": "https://www.grid.ac/institutes/grid.8073.c", 
          "name": [
            "Departamento de Biolox\u00eda Celular e Molecular, Facultade de Ciencias, Universidade da Coru\u00f1a, Campus da Zapateira s/n, 15071, A Coru\u00f1a, pSpain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gonz\u00e1lez Siso", 
        "givenName": "M.I.", 
        "id": "sg:person.011245364025.32", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011245364025.32"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/s0168-1656(00)00350-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000552849"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.82.23.7909", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001155767"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1097-0061(19980630)14:9<827::aid-yea281>3.0.co;2-n", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006224653"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1097-0061(19980630)14:9<827::aid-yea281>3.0.co;2-n", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006224653"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1026795706520", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007928027", 
          "https://doi.org/10.1023/a:1026795706520"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bit.260420319", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028164986"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/yea.320090110", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032284179"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/yea.320090110", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032284179"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1097-0290(19990920)64:6<692::aid-bit8>3.0.co;2-j", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036845878"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s002530050899", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046625019", 
          "https://doi.org/10.1007/s002530050899"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s002530051441", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048251685", 
          "https://doi.org/10.1007/s002530051441"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bit.260370614", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048797444"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/protein/14.5.379", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053313601"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2002-09", 
    "datePublishedReg": "2002-09-01", 
    "description": "A recombinant strain of Saccharomyces cerevisiae, secreting \u03b2-galactosidase from Kluyveromyces lactis, grew efficiently with more than 60 g lactose l\u22121. The growth rate (0.23 h\u22121) in a cheese-whey medium was close to the highest reported hitherto for other recombinant S. cerevisiae strains that express intracellular \u03b2-galactosidase and lactose-permease genes. The conditions for growth and \u03b2-galactosidase secretion in this medium were optimized in a series of factorial experiments. Best results were obtained at 23 \u00b0C for 72 h. Since the recombinant strain produced less than 3% ethanol from the lactose, it was also assayed for the production of fructose 1,6-bisphosphate from cheese whey, and 0.06 g l\u22121 h\u22121 were obtained.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1023/a:1019886112076", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1289037", 
        "issn": [
          "0951-208X", 
          "1573-6784"
        ], 
        "name": "Biotechnology Letters", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "17", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "24"
      }
    ], 
    "name": "Metabolic engineering for direct lactose utilization by Saccharomyces cerevisiae", 
    "pagination": "1391-1396", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "a024d7d3869f0a5e87b44e4230a9446b421f5427ddf31265e2f5fdf636ea01c4"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1023/a:1019886112076"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1033617873"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1023/a:1019886112076", 
      "https://app.dimensions.ai/details/publication/pub.1033617873"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T20:45", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8684_00000506.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1023%2FA%3A1019886112076"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1023/a:1019886112076'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1023/a:1019886112076'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1023/a:1019886112076'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1023/a:1019886112076'


 

This table displays all metadata directly associated to this object as RDF triples.

124 TRIPLES      21 PREDICATES      38 URIs      19 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1023/a:1019886112076 schema:about anzsrc-for:11
2 anzsrc-for:1103
3 schema:author Nfa9da1e4671f45659dd5a9e6bc424085
4 schema:citation sg:pub.10.1007/s002530050899
5 sg:pub.10.1007/s002530051441
6 sg:pub.10.1023/a:1026795706520
7 https://doi.org/10.1002/(sici)1097-0061(19980630)14:9<827::aid-yea281>3.0.co;2-n
8 https://doi.org/10.1002/(sici)1097-0290(19990920)64:6<692::aid-bit8>3.0.co;2-j
9 https://doi.org/10.1002/bit.260370614
10 https://doi.org/10.1002/bit.260420319
11 https://doi.org/10.1002/yea.320090110
12 https://doi.org/10.1016/s0168-1656(00)00350-3
13 https://doi.org/10.1073/pnas.82.23.7909
14 https://doi.org/10.1093/protein/14.5.379
15 schema:datePublished 2002-09
16 schema:datePublishedReg 2002-09-01
17 schema:description A recombinant strain of Saccharomyces cerevisiae, secreting β-galactosidase from Kluyveromyces lactis, grew efficiently with more than 60 g lactose l−1. The growth rate (0.23 h−1) in a cheese-whey medium was close to the highest reported hitherto for other recombinant S. cerevisiae strains that express intracellular β-galactosidase and lactose-permease genes. The conditions for growth and β-galactosidase secretion in this medium were optimized in a series of factorial experiments. Best results were obtained at 23 °C for 72 h. Since the recombinant strain produced less than 3% ethanol from the lactose, it was also assayed for the production of fructose 1,6-bisphosphate from cheese whey, and 0.06 g l−1 h−1 were obtained.
18 schema:genre research_article
19 schema:inLanguage en
20 schema:isAccessibleForFree false
21 schema:isPartOf Nac2acfc7dbde4cf6a4da78aa612bd464
22 Nae98d47f5e7d4f44a1ae599acc8ab0c7
23 sg:journal.1289037
24 schema:name Metabolic engineering for direct lactose utilization by Saccharomyces cerevisiae
25 schema:pagination 1391-1396
26 schema:productId N4fc56f927b6d457b884e55864168ed5e
27 N7365678053ec434c8ae02719584ed0be
28 Nbcb57ad7a45b4b3f9ffa681f7851ca6e
29 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033617873
30 https://doi.org/10.1023/a:1019886112076
31 schema:sdDatePublished 2019-04-10T20:45
32 schema:sdLicense https://scigraph.springernature.com/explorer/license/
33 schema:sdPublisher N42443cb17a684b11a7aa7b69ae36ef0b
34 schema:url http://link.springer.com/10.1023%2FA%3A1019886112076
35 sgo:license sg:explorer/license/
36 sgo:sdDataset articles
37 rdf:type schema:ScholarlyArticle
38 N26a41deef1fe494281ed0a776f0d1553 rdf:first sg:person.015730575154.33
39 rdf:rest N315b6a33efb34c1bb30318de7b196f2f
40 N315b6a33efb34c1bb30318de7b196f2f rdf:first sg:person.01312110053.66
41 rdf:rest Nf318ef187e0c4deea78cc845e94b27a6
42 N42443cb17a684b11a7aa7b69ae36ef0b schema:name Springer Nature - SN SciGraph project
43 rdf:type schema:Organization
44 N4fc56f927b6d457b884e55864168ed5e schema:name readcube_id
45 schema:value a024d7d3869f0a5e87b44e4230a9446b421f5427ddf31265e2f5fdf636ea01c4
46 rdf:type schema:PropertyValue
47 N7365678053ec434c8ae02719584ed0be schema:name dimensions_id
48 schema:value pub.1033617873
49 rdf:type schema:PropertyValue
50 N8673ae1685974865a80bc29a00416ec2 schema:affiliation https://www.grid.ac/institutes/grid.8073.c
51 schema:familyName Beccerra
52 schema:givenName M.
53 rdf:type schema:Person
54 Nac2acfc7dbde4cf6a4da78aa612bd464 schema:volumeNumber 24
55 rdf:type schema:PublicationVolume
56 Nae98d47f5e7d4f44a1ae599acc8ab0c7 schema:issueNumber 17
57 rdf:type schema:PublicationIssue
58 Nb1b5bb6a4972400d9d63ae4ce17be493 rdf:first sg:person.011245364025.32
59 rdf:rest rdf:nil
60 Nbcb57ad7a45b4b3f9ffa681f7851ca6e schema:name doi
61 schema:value 10.1023/a:1019886112076
62 rdf:type schema:PropertyValue
63 Nf318ef187e0c4deea78cc845e94b27a6 rdf:first sg:person.01302030267.03
64 rdf:rest Nb1b5bb6a4972400d9d63ae4ce17be493
65 Nfa9da1e4671f45659dd5a9e6bc424085 rdf:first N8673ae1685974865a80bc29a00416ec2
66 rdf:rest N26a41deef1fe494281ed0a776f0d1553
67 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
68 schema:name Medical and Health Sciences
69 rdf:type schema:DefinedTerm
70 anzsrc-for:1103 schema:inDefinedTermSet anzsrc-for:
71 schema:name Clinical Sciences
72 rdf:type schema:DefinedTerm
73 sg:journal.1289037 schema:issn 0951-208X
74 1573-6784
75 schema:name Biotechnology Letters
76 rdf:type schema:Periodical
77 sg:person.011245364025.32 schema:affiliation https://www.grid.ac/institutes/grid.8073.c
78 schema:familyName González Siso
79 schema:givenName M.I.
80 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011245364025.32
81 rdf:type schema:Person
82 sg:person.01302030267.03 schema:affiliation https://www.grid.ac/institutes/grid.8073.c
83 schema:familyName Cerdán
84 schema:givenName M.E.
85 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01302030267.03
86 rdf:type schema:Person
87 sg:person.01312110053.66 schema:affiliation https://www.grid.ac/institutes/grid.8073.c
88 schema:familyName Rodríguez-Belmonte
89 schema:givenName E.
90 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01312110053.66
91 rdf:type schema:Person
92 sg:person.015730575154.33 schema:affiliation https://www.grid.ac/institutes/grid.8073.c
93 schema:familyName Díaz Prado
94 schema:givenName S.
95 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015730575154.33
96 rdf:type schema:Person
97 sg:pub.10.1007/s002530050899 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046625019
98 https://doi.org/10.1007/s002530050899
99 rdf:type schema:CreativeWork
100 sg:pub.10.1007/s002530051441 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048251685
101 https://doi.org/10.1007/s002530051441
102 rdf:type schema:CreativeWork
103 sg:pub.10.1023/a:1026795706520 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007928027
104 https://doi.org/10.1023/a:1026795706520
105 rdf:type schema:CreativeWork
106 https://doi.org/10.1002/(sici)1097-0061(19980630)14:9<827::aid-yea281>3.0.co;2-n schema:sameAs https://app.dimensions.ai/details/publication/pub.1006224653
107 rdf:type schema:CreativeWork
108 https://doi.org/10.1002/(sici)1097-0290(19990920)64:6<692::aid-bit8>3.0.co;2-j schema:sameAs https://app.dimensions.ai/details/publication/pub.1036845878
109 rdf:type schema:CreativeWork
110 https://doi.org/10.1002/bit.260370614 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048797444
111 rdf:type schema:CreativeWork
112 https://doi.org/10.1002/bit.260420319 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028164986
113 rdf:type schema:CreativeWork
114 https://doi.org/10.1002/yea.320090110 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032284179
115 rdf:type schema:CreativeWork
116 https://doi.org/10.1016/s0168-1656(00)00350-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000552849
117 rdf:type schema:CreativeWork
118 https://doi.org/10.1073/pnas.82.23.7909 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001155767
119 rdf:type schema:CreativeWork
120 https://doi.org/10.1093/protein/14.5.379 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053313601
121 rdf:type schema:CreativeWork
122 https://www.grid.ac/institutes/grid.8073.c schema:alternateName University of A Coruña
123 schema:name Departamento de Bioloxía Celular e Molecular, Facultade de Ciencias, Universidade da Coruña, Campus da Zapateira s/n, 15071, A Coruña, pSpain
124 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...