Transport of Human Recombinant Brain-Derived Neurotrophic Factor (BDNF) Through the Rat Blood−Brain Barrier in Vivo Using Vector-Mediated Peptide Drug Delivery View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1994-05

AUTHORS

William M. Pardridge, Young-Sook Kang, Jody L. Buciak

ABSTRACT

The blood-brain barrier (BBB) transport of brain-derived neurotrophic factor (BDNF) in anesthetized rats was examined in the present studies using vector-mediated peptide drug delivery. Following tritiation, the BDNF was biotinylated via a disulfide linker and was coupled to a covalent conjugate of neutral avidin (NLA), which binds the biotinylated peptide with a high affinity, and the murine OX26 monoclonal antibody to the rat transferrin receptor. Owing to the abundance of transferrin receptors on brain capillary endothelium, the OX26 monoclonal antibody undergoes receptor-mediated transcytosis through the BBB, and the NLA-OX26 conjugate transports biotinylated peptide therapeutics through the BBB. The present studies show that while unconjugated BDNF was not transported through the BBB in vivo, the conjugation of biotinylated BDNF to the NLA-OX26 vector resulted in a marked increase in the brain delivery of BDNF, as defined by measurements of the percentage of the injected dose (ID) delivered per gram of brain. Although BDNF was not transported through the BBB in vivo, this cationic peptide was avidly bound by isolated human brain capillaries via a low-affinity, high-capacity system that was inhibited by protamine and by serum protein binding of BDNF. In conclusion, these studies show that the delivery of unconjugated BDNF to brain is nil owing to the combined effects of negligible BBB transport and rapid systemic clearance of intravenous administered BDNF. The brain delivery of BDNF may be augmented by conjugation of BDNF to BBB drug delivery vectors, such as the NLA-OX26 conjugate. More... »

PAGES

738-746

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1018940732550

DOI

http://dx.doi.org/10.1023/a:1018940732550

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1031703583

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/8058646


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1109", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Neurosciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antibodies, Monoclonal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Avidin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biological Availability", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biotin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Blood-Brain Barrier", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Brain-Derived Neurotrophic Factor", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Capillaries", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Carrier Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromatography, Gel", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromatography, High Pressure Liquid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Disulfides", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "In Vitro Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nerve Tissue Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rats", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rats, Sprague-Dawley", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombinant Proteins", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of California Los Angeles", 
          "id": "https://www.grid.ac/institutes/grid.19006.3e", 
          "name": [
            "Department of Medicine, Brain Research Institute, UCLA School of Medicine, 90024, Los Angeles, California"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pardridge", 
        "givenName": "William M.", 
        "id": "sg:person.0725622754.91", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0725622754.91"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of California Los Angeles", 
          "id": "https://www.grid.ac/institutes/grid.19006.3e", 
          "name": [
            "Department of Medicine, Brain Research Institute, UCLA School of Medicine, 90024, Los Angeles, California"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kang", 
        "givenName": "Young-Sook", 
        "id": "sg:person.01177022730.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01177022730.35"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of California Los Angeles", 
          "id": "https://www.grid.ac/institutes/grid.19006.3e", 
          "name": [
            "Department of Medicine, Brain Research Institute, UCLA School of Medicine, 90024, Los Angeles, California"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Buciak", 
        "givenName": "Jody L.", 
        "id": "sg:person.010124405237.55", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010124405237.55"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1172/jci116825", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001075012"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.89.23.11249", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008693278"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01190839", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022764296", 
          "https://doi.org/10.1007/bf01190839"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01190839", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022764296", 
          "https://doi.org/10.1007/bf01190839"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/350230a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026248816", 
          "https://doi.org/10.1038/350230a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0896-6273(90)90166-d", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026630969"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/341149a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027380263", 
          "https://doi.org/10.1038/341149a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1471-4159.1992.tb08880.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030741344"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.89.2.648", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032868553"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejm197711172972005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034908396"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.88.3.961", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037323886"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.89.23.11347", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042595895"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1471-4159.1986.tb13015.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044366303"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1471-4159.1985.tb07167.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047740530"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.90.7.2618", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050463584"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0026-0495(90)90251-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050684213"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.87.20.8060", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053215041"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/sqb.1990.055.01.038", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060406243"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1688328", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062500207"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.8420006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062655511"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1210/endo-129-3-1289", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1064264553"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1076429040", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077083895", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082674410", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082683953", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1994-05", 
    "datePublishedReg": "1994-05-01", 
    "description": "The blood-brain barrier (BBB) transport of brain-derived neurotrophic factor (BDNF) in anesthetized rats was examined in the present studies using vector-mediated peptide drug delivery. Following tritiation, the BDNF was biotinylated via a disulfide linker and was coupled to a covalent conjugate of neutral avidin (NLA), which binds the biotinylated peptide with a high affinity, and the murine OX26 monoclonal antibody to the rat transferrin receptor. Owing to the abundance of transferrin receptors on brain capillary endothelium, the OX26 monoclonal antibody undergoes receptor-mediated transcytosis through the BBB, and the NLA-OX26 conjugate transports biotinylated peptide therapeutics through the BBB. The present studies show that while unconjugated BDNF was not transported through the BBB in vivo, the conjugation of biotinylated BDNF to the NLA-OX26 vector resulted in a marked increase in the brain delivery of BDNF, as defined by measurements of the percentage of the injected dose (ID) delivered per gram of brain. Although BDNF was not transported through the BBB in vivo, this cationic peptide was avidly bound by isolated human brain capillaries via a low-affinity, high-capacity system that was inhibited by protamine and by serum protein binding of BDNF. In conclusion, these studies show that the delivery of unconjugated BDNF to brain is nil owing to the combined effects of negligible BBB transport and rapid systemic clearance of intravenous administered BDNF. The brain delivery of BDNF may be augmented by conjugation of BDNF to BBB drug delivery vectors, such as the NLA-OX26 conjugate.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1023/a:1018940732550", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2483561", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1094644", 
        "issn": [
          "0724-8741", 
          "1573-904X"
        ], 
        "name": "Pharmaceutical Research", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "11"
      }
    ], 
    "name": "Transport of Human Recombinant Brain-Derived Neurotrophic Factor (BDNF) Through the Rat Blood\u2212Brain Barrier in Vivo Using Vector-Mediated Peptide Drug Delivery", 
    "pagination": "738-746", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "97079525895dc79944eaca1b95f6d7ae358f86c6bf535721c225ea90504597d2"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "8058646"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "8406521"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1023/a:1018940732550"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1031703583"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1023/a:1018940732550", 
      "https://app.dimensions.ai/details/publication/pub.1031703583"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T19:07", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8678_00000506.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1023%2FA%3A1018940732550"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1023/a:1018940732550'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1023/a:1018940732550'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1023/a:1018940732550'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1023/a:1018940732550'


 

This table displays all metadata directly associated to this object as RDF triples.

232 TRIPLES      21 PREDICATES      72 URIs      40 LITERALS      28 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1023/a:1018940732550 schema:about N154742fed79545f38e286a8eb24bb1aa
2 N3564c94cce8a407b991e2a8bf6b04102
3 N41d33ee24690413fa978226802eefaa2
4 N5406709f3224456dba01b1114924dd53
5 N6a0844d13b30498e88a85635a150f7e4
6 N72b760791912487487194f1fa38a99cb
7 N90e2d82800894735904d4a6031aa8896
8 N9aa3c55beb0e4c5ca70d79e76e6c5bce
9 N9d1b0dac67814b79888df6ac3206aac0
10 Nb22215268a9447a88c868c3e2bba2199
11 Nbc635c74f6d44abba4fc15f1a414c2ef
12 Nc164dd2335494bcdb626a8d43d627213
13 Nc871435cb2f2420abd789dd87c81b263
14 Ncf808d283e2c4d31a357500528fbdf98
15 Nd5c26b806c6f4762ae0ca1213cd87d38
16 Nd772578edcea4f75a24931e1b3499bb2
17 Ndfb34f94eee94c1691dc11e5b4b6c9b6
18 Ne1c6f101e2d94485b2eafed1995934a3
19 Ne4ccb23ce5ff470f82d65be7a51d2c90
20 anzsrc-for:11
21 anzsrc-for:1109
22 schema:author N8595636bb3fc40e7b821f532355c01e6
23 schema:citation sg:pub.10.1007/bf01190839
24 sg:pub.10.1038/341149a0
25 sg:pub.10.1038/350230a0
26 https://app.dimensions.ai/details/publication/pub.1076429040
27 https://app.dimensions.ai/details/publication/pub.1077083895
28 https://app.dimensions.ai/details/publication/pub.1082674410
29 https://app.dimensions.ai/details/publication/pub.1082683953
30 https://doi.org/10.1016/0026-0495(90)90251-7
31 https://doi.org/10.1016/0896-6273(90)90166-d
32 https://doi.org/10.1056/nejm197711172972005
33 https://doi.org/10.1073/pnas.87.20.8060
34 https://doi.org/10.1073/pnas.88.3.961
35 https://doi.org/10.1073/pnas.89.2.648
36 https://doi.org/10.1073/pnas.89.23.11249
37 https://doi.org/10.1073/pnas.89.23.11347
38 https://doi.org/10.1073/pnas.90.7.2618
39 https://doi.org/10.1101/sqb.1990.055.01.038
40 https://doi.org/10.1111/j.1471-4159.1985.tb07167.x
41 https://doi.org/10.1111/j.1471-4159.1986.tb13015.x
42 https://doi.org/10.1111/j.1471-4159.1992.tb08880.x
43 https://doi.org/10.1126/science.1688328
44 https://doi.org/10.1126/science.8420006
45 https://doi.org/10.1172/jci116825
46 https://doi.org/10.1210/endo-129-3-1289
47 schema:datePublished 1994-05
48 schema:datePublishedReg 1994-05-01
49 schema:description The blood-brain barrier (BBB) transport of brain-derived neurotrophic factor (BDNF) in anesthetized rats was examined in the present studies using vector-mediated peptide drug delivery. Following tritiation, the BDNF was biotinylated via a disulfide linker and was coupled to a covalent conjugate of neutral avidin (NLA), which binds the biotinylated peptide with a high affinity, and the murine OX26 monoclonal antibody to the rat transferrin receptor. Owing to the abundance of transferrin receptors on brain capillary endothelium, the OX26 monoclonal antibody undergoes receptor-mediated transcytosis through the BBB, and the NLA-OX26 conjugate transports biotinylated peptide therapeutics through the BBB. The present studies show that while unconjugated BDNF was not transported through the BBB in vivo, the conjugation of biotinylated BDNF to the NLA-OX26 vector resulted in a marked increase in the brain delivery of BDNF, as defined by measurements of the percentage of the injected dose (ID) delivered per gram of brain. Although BDNF was not transported through the BBB in vivo, this cationic peptide was avidly bound by isolated human brain capillaries via a low-affinity, high-capacity system that was inhibited by protamine and by serum protein binding of BDNF. In conclusion, these studies show that the delivery of unconjugated BDNF to brain is nil owing to the combined effects of negligible BBB transport and rapid systemic clearance of intravenous administered BDNF. The brain delivery of BDNF may be augmented by conjugation of BDNF to BBB drug delivery vectors, such as the NLA-OX26 conjugate.
50 schema:genre research_article
51 schema:inLanguage en
52 schema:isAccessibleForFree false
53 schema:isPartOf N449576da2645423a959b1270051e8e79
54 Ne99c1dd5d6be41d3b8dba23f383b11a5
55 sg:journal.1094644
56 schema:name Transport of Human Recombinant Brain-Derived Neurotrophic Factor (BDNF) Through the Rat Blood−Brain Barrier in Vivo Using Vector-Mediated Peptide Drug Delivery
57 schema:pagination 738-746
58 schema:productId N08af13802c4d4609b2fe89bf5b86d34c
59 N3aad9566be184658ab923381907390b0
60 N9703ab0d99a04eeb858b3ca94e617b7a
61 Ncc730ea2db5e487687156118083d9ab3
62 Nd9e58f3443044e139567fd971e431157
63 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031703583
64 https://doi.org/10.1023/a:1018940732550
65 schema:sdDatePublished 2019-04-10T19:07
66 schema:sdLicense https://scigraph.springernature.com/explorer/license/
67 schema:sdPublisher Nba9f5f76184c470a86d104451f0a2a25
68 schema:url http://link.springer.com/10.1023%2FA%3A1018940732550
69 sgo:license sg:explorer/license/
70 sgo:sdDataset articles
71 rdf:type schema:ScholarlyArticle
72 N08af13802c4d4609b2fe89bf5b86d34c schema:name readcube_id
73 schema:value 97079525895dc79944eaca1b95f6d7ae358f86c6bf535721c225ea90504597d2
74 rdf:type schema:PropertyValue
75 N154742fed79545f38e286a8eb24bb1aa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
76 schema:name Brain-Derived Neurotrophic Factor
77 rdf:type schema:DefinedTerm
78 N3564c94cce8a407b991e2a8bf6b04102 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name Biological Availability
80 rdf:type schema:DefinedTerm
81 N3aad9566be184658ab923381907390b0 schema:name doi
82 schema:value 10.1023/a:1018940732550
83 rdf:type schema:PropertyValue
84 N41d33ee24690413fa978226802eefaa2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Humans
86 rdf:type schema:DefinedTerm
87 N449576da2645423a959b1270051e8e79 schema:issueNumber 5
88 rdf:type schema:PublicationIssue
89 N5406709f3224456dba01b1114924dd53 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Biotin
91 rdf:type schema:DefinedTerm
92 N61a6f5c689be4c44b1204b0426d509e4 rdf:first sg:person.01177022730.35
93 rdf:rest Nac1193ea48f84805bac45ce957f98f71
94 N6a0844d13b30498e88a85635a150f7e4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
95 schema:name In Vitro Techniques
96 rdf:type schema:DefinedTerm
97 N72b760791912487487194f1fa38a99cb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Male
99 rdf:type schema:DefinedTerm
100 N8595636bb3fc40e7b821f532355c01e6 rdf:first sg:person.0725622754.91
101 rdf:rest N61a6f5c689be4c44b1204b0426d509e4
102 N90e2d82800894735904d4a6031aa8896 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Animals
104 rdf:type schema:DefinedTerm
105 N9703ab0d99a04eeb858b3ca94e617b7a schema:name pubmed_id
106 schema:value 8058646
107 rdf:type schema:PropertyValue
108 N9aa3c55beb0e4c5ca70d79e76e6c5bce schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Rats, Sprague-Dawley
110 rdf:type schema:DefinedTerm
111 N9d1b0dac67814b79888df6ac3206aac0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Capillaries
113 rdf:type schema:DefinedTerm
114 Nac1193ea48f84805bac45ce957f98f71 rdf:first sg:person.010124405237.55
115 rdf:rest rdf:nil
116 Nb22215268a9447a88c868c3e2bba2199 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Recombinant Proteins
118 rdf:type schema:DefinedTerm
119 Nba9f5f76184c470a86d104451f0a2a25 schema:name Springer Nature - SN SciGraph project
120 rdf:type schema:Organization
121 Nbc635c74f6d44abba4fc15f1a414c2ef schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
122 schema:name Carrier Proteins
123 rdf:type schema:DefinedTerm
124 Nc164dd2335494bcdb626a8d43d627213 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Disulfides
126 rdf:type schema:DefinedTerm
127 Nc871435cb2f2420abd789dd87c81b263 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name Avidin
129 rdf:type schema:DefinedTerm
130 Ncc730ea2db5e487687156118083d9ab3 schema:name dimensions_id
131 schema:value pub.1031703583
132 rdf:type schema:PropertyValue
133 Ncf808d283e2c4d31a357500528fbdf98 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Blood-Brain Barrier
135 rdf:type schema:DefinedTerm
136 Nd5c26b806c6f4762ae0ca1213cd87d38 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Rats
138 rdf:type schema:DefinedTerm
139 Nd772578edcea4f75a24931e1b3499bb2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Chromatography, High Pressure Liquid
141 rdf:type schema:DefinedTerm
142 Nd9e58f3443044e139567fd971e431157 schema:name nlm_unique_id
143 schema:value 8406521
144 rdf:type schema:PropertyValue
145 Ndfb34f94eee94c1691dc11e5b4b6c9b6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Nerve Tissue Proteins
147 rdf:type schema:DefinedTerm
148 Ne1c6f101e2d94485b2eafed1995934a3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
149 schema:name Chromatography, Gel
150 rdf:type schema:DefinedTerm
151 Ne4ccb23ce5ff470f82d65be7a51d2c90 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name Antibodies, Monoclonal
153 rdf:type schema:DefinedTerm
154 Ne99c1dd5d6be41d3b8dba23f383b11a5 schema:volumeNumber 11
155 rdf:type schema:PublicationVolume
156 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
157 schema:name Medical and Health Sciences
158 rdf:type schema:DefinedTerm
159 anzsrc-for:1109 schema:inDefinedTermSet anzsrc-for:
160 schema:name Neurosciences
161 rdf:type schema:DefinedTerm
162 sg:grant.2483561 http://pending.schema.org/fundedItem sg:pub.10.1023/a:1018940732550
163 rdf:type schema:MonetaryGrant
164 sg:journal.1094644 schema:issn 0724-8741
165 1573-904X
166 schema:name Pharmaceutical Research
167 rdf:type schema:Periodical
168 sg:person.010124405237.55 schema:affiliation https://www.grid.ac/institutes/grid.19006.3e
169 schema:familyName Buciak
170 schema:givenName Jody L.
171 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010124405237.55
172 rdf:type schema:Person
173 sg:person.01177022730.35 schema:affiliation https://www.grid.ac/institutes/grid.19006.3e
174 schema:familyName Kang
175 schema:givenName Young-Sook
176 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01177022730.35
177 rdf:type schema:Person
178 sg:person.0725622754.91 schema:affiliation https://www.grid.ac/institutes/grid.19006.3e
179 schema:familyName Pardridge
180 schema:givenName William M.
181 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0725622754.91
182 rdf:type schema:Person
183 sg:pub.10.1007/bf01190839 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022764296
184 https://doi.org/10.1007/bf01190839
185 rdf:type schema:CreativeWork
186 sg:pub.10.1038/341149a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027380263
187 https://doi.org/10.1038/341149a0
188 rdf:type schema:CreativeWork
189 sg:pub.10.1038/350230a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026248816
190 https://doi.org/10.1038/350230a0
191 rdf:type schema:CreativeWork
192 https://app.dimensions.ai/details/publication/pub.1076429040 schema:CreativeWork
193 https://app.dimensions.ai/details/publication/pub.1077083895 schema:CreativeWork
194 https://app.dimensions.ai/details/publication/pub.1082674410 schema:CreativeWork
195 https://app.dimensions.ai/details/publication/pub.1082683953 schema:CreativeWork
196 https://doi.org/10.1016/0026-0495(90)90251-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050684213
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1016/0896-6273(90)90166-d schema:sameAs https://app.dimensions.ai/details/publication/pub.1026630969
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1056/nejm197711172972005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034908396
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1073/pnas.87.20.8060 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053215041
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1073/pnas.88.3.961 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037323886
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1073/pnas.89.2.648 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032868553
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1073/pnas.89.23.11249 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008693278
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1073/pnas.89.23.11347 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042595895
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1073/pnas.90.7.2618 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050463584
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1101/sqb.1990.055.01.038 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060406243
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1111/j.1471-4159.1985.tb07167.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1047740530
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1111/j.1471-4159.1986.tb13015.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1044366303
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1111/j.1471-4159.1992.tb08880.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1030741344
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1126/science.1688328 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062500207
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1126/science.8420006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062655511
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1172/jci116825 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001075012
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1210/endo-129-3-1289 schema:sameAs https://app.dimensions.ai/details/publication/pub.1064264553
229 rdf:type schema:CreativeWork
230 https://www.grid.ac/institutes/grid.19006.3e schema:alternateName University of California Los Angeles
231 schema:name Department of Medicine, Brain Research Institute, UCLA School of Medicine, 90024, Los Angeles, California
232 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...