Sodium Butyrate-Induced Liver-Type Alkaline Phosphatase Activity in a Small Intestinal Epithelial Cell Line, IEC6 View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-05

AUTHORS

Kouhei Fukushima, Iwao Sasaki, Hideaki Hasegawa, Ken-Ichi Takahashi, Hiroo Naito, Yuji Funayama, Seiki Matsuno

ABSTRACT

Sodium butyrate is a well-recognizeddifferentiating agent inducing alkaline phosphataseactivity, one of the epithelial differentiation markers.When IEC6 cells, a nontransformed, small intestinalepithelial cell line, were cultured with butyrate, thissubstrate induced alkaline phosphate activity in a time-and dose-dependent fashion. However, the type ofisoenzyme involved was a liver-type, not anintestinal-type. Electron microscopy revealed that the inducedactivity was strictly localized in the cytosol and noton the plasma membrane. However, disaccharidaseactivities, another kind of differentiation marker, were also enhanced by sodium butyrate. In addition,the positive cells demonstrating the presence ofalkaline phosphatase activity were preferentiallyobserved in tubular structures. These data show thatbutyrate-induced alkaline phosphatase activity is closelyassociated with differentiation-like phenomena in IEC6cells, although the type of isoenzyme and cellularlocalization of the activity are different from thoseobserved in mucosa. More... »

PAGES

1116-1123

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1018807524075

DOI

http://dx.doi.org/10.1023/a:1018807524075

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1011626093

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/9590430


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