Fast-HMQC using Ernst angle pulses: An efficient tool for screening of ligand binding to target proteins View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1997-12

AUTHORS

Alfred Ross, Michael Salzmann, Hans Senn

ABSTRACT

The application of Ernst angle pulses in multidimensional NMR spectroscopy is theoretically and experimentally investigated. Theory shows that only for a few pulse sequences employed at high repetition rate, a remarkable gain in sensitivity is possible using Ernst angle pulses. As an example, a new variant of the heteronuclear multiple quantum coherence (HMQC) experiment, the fast-((1)H,(15)N)-HMQC, is described. This sequence allows, with a 1 mM protein sample in H(2)O, the acquisition of a highly resolved two-dimensional ((1)H,(15)N) correlated spectrum within 37 s. The high efficiency of the fast-HMQC to detect ligand binding to a target protein is demonstrated. More... »

PAGES

389-396

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1018361214472

DOI

http://dx.doi.org/10.1023/a:1018361214472

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1011806155

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/20859783


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