Induction of microspore embryos in a CMS line of Brassica juncea and formation of the androgenic plantlets View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2001-07

AUTHORS

Meghna Rana Malik, N.S. Rangaswamy, K.R. Shivanna

ABSTRACT

Anther culture of two wide hybrids (Diplotaxis erucoides × Brassica campestris) × B. juncea and (D. berthautii × B. campestris) × B. juncea, their CMS lines and the parent species elicited a range of responses highlighting the importance of the genotype. Androgenesis was expressed in cultured anthers of CMS (D. erucoides) B. juncea (22.8%), in restored pollen fertile plants of this CMS line (1.66%), and in the parent, B. juncea cv Pusa Bold (13.02%). AgNO3 was essential for androgenic response in the CMS lines, and it markedly increased the frequency of androgenesis in the cultivated species. Multiple crops of microspore embryos were obtained from responsive anthers of CMS plants in anther recultures. As high as92% microspore embryos of the CMS line germinated on basal B5medium and formed normal plantlets. More... »

PAGES

195-203

Journal

TITLE

Euphytica

ISSUE

2

VOLUME

120

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1017502113066

DOI

http://dx.doi.org/10.1023/a:1017502113066

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1001799361


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