Separation and Structure-Function Studies of Taiwan Cobra Cardiotoxins View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2002-02

AUTHORS

Shinne-Ren Lin, Long-Sen Chang, Kee-Lung Chang

ABSTRACT

Six cardiotoxins (CTXs) and one cardiotoxin-like basic protein (CLBP) from Naja naja atra (Taiwan cobra) venom were separated by a SP-Sephadex C-25 column. CTXn and CTXI were well separated by eluting with ammonium acetate buffer, and the separation of CLBP from CTXIV and CTXV mixtures was achieved using sodium phosphate buffer. These findings suggest a differential interaction of CTXs with the chromatographic matrix using different buffer systems. Chemical modification studies on cationic residues of CTXI suggested that there was no single lysine or arginine residue exclusively responsible for its biological activity. Moreover, it was found that the cytotoxicity and hemolytic sites of CTXI could be dissociated by chemical modifications. It suggests the potentiality for preparing toxin derivatives in which a specific activity is retained. More... »

PAGES

81-86

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1014520126856

DOI

http://dx.doi.org/10.1023/a:1014520126856

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1006105320

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/11934278


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