Identification of a soluble form of the angiopoietin receptor TIE-2 released from endothelial cells and present in human blood View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2001-06

AUTHORS

Petra Reusch, Bernhard Barleon, Karin Weindel, Georg Martiny-Baron, Astrid Gödde, Gerhard Siemeister, Dieter Marmé

ABSTRACT

The transmembrane tyrosine kinase TIE-2, the receptor for the angiopoietins-1 and -2, has been shown to be involved in angiogenic processes. Investigating the regulation of TIE-2 expression on endothelial cells, we found that stimulators such as PMA induce a decrease of TIE-2 protein from the cell surface without affecting TIE-2 mRNA. In conditioned media of PMA stimulated endothelial cells, a soluble form of this receptor comprising parts of the extracellular domain can be detected. Using a sandwich ELISA, we were able to detect and quantify TIE-2 receptors in cell lysates (representing the whole transmembrane receptor) and in cell culture supernatants (representing a soluble form of this receptor, sTIE-2). Several factors influencing the shedding process e.g. basic FGF could be identified. Finally, the soluble form of TIE-2 could also be detected in human biological fluids such as sera and plasma from healthy controls. More... »

PAGES

123-131

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1012226627813

DOI

http://dx.doi.org/10.1023/a:1012226627813

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1050272920

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/11806244


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