Ontology type: schema:ScholarlyArticle
1997-09
AUTHORSAkiko Kozu, Yukio Kato, Yoshihisa Shitara, Manabu Hanano, Yuichi Sugiyama
ABSTRACTPurpose. The aim of this study is to clarify the intracellular fate and a rate limiting step in transcytosis of epidermal growth factor (EGF) in Madin-Darby Canine Kidney (MDCK) epithelial cells.Methods. The kinetics of transcytosis of 125I-EGF was investigated. To examine the fate of EGF molecules bound to its receptor on the cell surface, 125I-EGF was prebound to the basal surface at 4°C, followed by extensive washing and subsequent incubation at 37°C in EGF-free medium.Results. Saturable transport of 125I-EGF through the cell monolayer could only be observed from the basal to apical side. Most (~90%) of the EGF molecules bound to the surface receptor are internalized with a half-life of 1−3 min, followed by intracellular degradation with a half-life of 20−50 min. The exocytosis of internalized EGF into the apical medium is much slower with a half-life of 130−250 min. Even when 125I-EGF was incubated with MDCK cells at 37°C and washed with acid to remove cell-surface 125I-EGF, intact 125I-EGF appeared in the basal medium with a half life of 160−170 min.Conclusions. The exocytosis of internalized EGF into the apical medium is a rate limiting step in EGF transcytosis. At least a small amount of internalized EGF is recycled. More... »
PAGES1228-1235
http://scigraph.springernature.com/pub.10.1023/a:1012167126364
DOIhttp://dx.doi.org/10.1023/a:1012167126364
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PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/9327453
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