The gene family coding for the light-harvesting polypeptides of Photosystem I of the red alga Galdieria sulphuraria* View Full Text


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Article Info

DATE

2001-05

AUTHORS

Jürgen Marquardt, Bodo Lutz, Stefan Wans, Erhard Rhiel, Wolfgang E. Krumbein

ABSTRACT

Recently [Marquardt et al. (2000) Gene 255: 257-265], we isolated a gene encoding a polypeptide of the light-harvesting complex of Photosystem I (LHC I) of the red alga Galdieria sulphuraria. By screening a G. sulphuraria cDNA library with a DNA probe coding for the conserved first transmembrane helix of this protein we isolated four additional genes coding for LHC I polypeptides. The deduced preproteins had calculated molecular masses of 24.6-25.6 kDa and isoelectric points of 8.09-9.82. N-terminal sequencing of a LHC I polypeptide isolated by gel electrophoresis allowed us to determine the cleavage site of the transit peptide of one of the deduced polypeptides. The mature protein has a calculated molecular mass of 20.6 kDa and an isoelectric point of 7.76. The genes were amplified from nuclear G. sulphuraria DNA by polymerase chain reaction (PCR) using oligonucleotides annealing in the regions of the start and stop codons as primers. All genomic sequences were 80-300 base pairs longer than the PCR products obtained from the respective cDNA clones, pointing to the existence of 1-5 introns per gene. The G. sulphuraria genes form a homogeneous gene family with overall pairwise amino acid identities of 46.0-56.6%. Homology to two diatom, one cryptophytic and two higher plant light-harvesting polypeptides was lower with pairwise identities of 21.1-34.1%. Only one diatom polypeptide showed a higher degree of identity of up to -39.3%. More... »

PAGES

121-130

References to SciGraph publications

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  • 1999-09. A Proposal for Extending the Nomenclature of Light-Harvesting Proteins of the Three Transmembrane Helix Type in PLANT MOLECULAR BIOLOGY REPORTER
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  • 1998-12. The genes encoding light-harvesting subunits of Cyclotella cryptica (Bacillariophyceae) constitute a complex and heterogeneous family in MOLECULAR GENETICS AND GENOMICS
  • 1995-07. Decrease of polypeptides in the PS I antenna complex with increasing growth irradiance in the red alga Porphyridium cruentum in PHOTOSYNTHESIS RESEARCH
  • 1999-01. A Phylogenetic Assessment of the Eukaryotic Light-Harvesting Antenna Proteins, with Implications for Plastid Evolution in JOURNAL OF MOLECULAR EVOLUTION
  • 1994-02. Hypothesis for the evolution of three-helix Chl a/b and Chl a/c light-harvesting antenna proteins from two-helix and four-helix ancestors in PHOTOSYNTHESIS RESEARCH
  • 1997-09. Red algal LHC I genes have similarities with both Chl a/b- and a/c-binding proteins: A 21 kDa polypeptide encoded by LhcaR2 is one of the six LHC I polypeptides in PHOTOSYNTHESIS RESEARCH
  • 1970-08. Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 in NATURE
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    http://scigraph.springernature.com/pub.10.1023/a:1011865415369

    DOI

    http://dx.doi.org/10.1023/a:1011865415369

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/16228335


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