Development of Ac and Ds transposon tagging lines for gene isolation in diploid potato View Full Text


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Article Info

DATE

2001-06

AUTHORS

L. (Ellen) J.G. van Enckevort, Jan Lasschuit, Willem J. Stiekema, Evert Jacobsen, Andy Pereira

ABSTRACT

For the development of an efficient transposon tagging strategy it is important to generate populations of plants containing unique independent transposon insertions that will mutate genes of interest. To develop such a transposon system in diploid potato the behavior of the autonomous maize transposable element Ac and the mobile Ds element was studied. A GBSS (Waxy) excision assay developed for Ac was used to monitor excision in somatic starch-forming tissue like tubers and pollen. Excision of Ac results in production of amylose starch that stains blue with iodine. The frequency and patterns of blue staining starch granules on tuber slices enabled the identification of transformants with different Ac activity. After excision the GBSS complementation was usually not complete, probably due to the segment of DNA flanking Ac that is left behind in the GBSS gene. Molecular and phenotypic analyses of 40 primary transformants classified into 4 phenotypic classes revealed reproducible patterns. A very high percentage (32.5%) of the primary transformants clearly showed early excision in the first transformed cell as displayed both by the analysis of the GBSS excision marker gene as well as DNA blot analyses. Genotypes useful for tagging strategies were used for crosses and the frequency of independent germinal transpositions was assessed. In crosses to Ds genotypes, excision of Ds was revealed that correlated to the activity of the Ac genotype. A line displaying Ac amplification to multiple copies conferred a high frequency of independent Ds transpositions. The genotypes described here are useful in somatic insertion mutagenesis aimed at the isolation of tagged mutations in diploid potato. More... »

PAGES

117-129

References to SciGraph publications

  • 1988-08. Transposition of the maize transposable element Ac in Solanum tuberosum in MOLECULAR GENETICS AND GENOMICS
  • 1989-11. Phenotypic and genotypic characterization of an amylose-free starch mutant of the potato in EUPHYTICA
  • 1991-08. Sequence of the structural gene for granule-bound starch synthase of potato (Solarium tuberosum L.) and evidence for a single point deletion in the amf allele in MOLECULAR GENETICS AND GENOMICS
  • 1968-04. Die Plastidenzahl als Merkmal bei der Kartoffel in THEORETICAL AND APPLIED GENETICS
  • 1989-07. The maize transposable element Ac excises in progeny of transformed tobacco in PLANT MOLECULAR BIOLOGY
  • 1989-03. Transformation of homozygous diploid potato with an Agrobacterium tumefaciens binary vector system by adventitious shoot regeneration on leaf and stem segments in PLANT MOLECULAR BIOLOGY
  • 1964-09. Self-compatibility in hybrids between Phureja and haploid Andigena clones ofSolanum tuberosum in POTATO RESEARCH
  • 1994-06. Expression of a wild-type GBSS gene introduced into an amylose-free potato mutant by Agrobacterium tumefaciens and the inheritance of the inserts at the microsporic level in THEORETICAL AND APPLIED GENETICS
  • 2000-09. Selection of independent Ds transposon insertions in somatic tissue of potato by protoplast regeneration in THEORETICAL AND APPLIED GENETICS
  • 1984-04. Cloning of the Zea mays controlling element Ac from the wx-m7 allele in MOLECULAR GENETICS AND GENOMICS
  • 1971-06. Inbreeding in cultivated diploid potatoes in POTATO RESEARCH
  • 1995-09. Genetic localisation of transformation competence in diploid potato in THEORETICAL AND APPLIED GENETICS
  • 1994-03. Segregation analysis and RFLP mapping of the R1 and R3 alleles conferring race-specific resistance to Phytophthora infestans in progeny of dihaploid potato parents in MOLECULAR GENETICS AND GENOMICS
  • 1987-12. Isolation of an amylose-free starch mutant of the potato (Solanum tuberosum L.) in THEORETICAL AND APPLIED GENETICS
  • 1986-09. The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vector in MOLECULAR GENETICS AND GENOMICS
  • 1992-03. Towards the isolation of resistance genes by transposon targeting in potato in EUROPEAN JOURNAL OF PLANT PATHOLOGY
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    http://scigraph.springernature.com/pub.10.1023/a:1011362323992

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    http://dx.doi.org/10.1023/a:1011362323992

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    https://app.dimensions.ai/details/publication/pub.1038886987


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