Y-ligation: An efficient method for ligating single-stranded DNAs and RNAs with T4 RNA ligase View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-09

AUTHORS

Koichi Nishigaki, Katsuya Taguchi, Yasunori Kinoshita, Takuyo Aita, Yuzuru Husimi

ABSTRACT

Very efficient ligation of oligodeoxyribonucleotides was attained through a simple molecular construct, which is composed of one stem and two branches (Y-shape), with use of T4 RNA ligase. Single-stranded DNAs (naturally, RNAs also) of more than 100 nucleotides (even 800 nts) were considerably ligated, approximately as theoretically expected. Owing to the molecular construct adopted, such a tiny amount of ligation products could be amplified to a sufficient amount by PCR and then recovered as single-stranded DNAs. This advantage of being amplifiable is shown to be useful for both combinatorial chemistry and evolutionary molecular engineering, which deal with a pool of diversity molecules. More... »

PAGES

187-190

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1009644028931

DOI

http://dx.doi.org/10.1023/a:1009644028931

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1000949409

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/10729904


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