Transgenic barley expressing a fungal xylanase gene in the endosperm of the developing grains View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-02

AUTHORS

Minesh Patel, Jennifer S. Johnson, Richard I.S. Brettell, Jake Jacobsen, Gang-Ping Xue

ABSTRACT

The feasibility of producing plant cell wall polysaccharide-hydrolysing feed enzymes in the endosperm of barley grain was investigated. The coding region of a modified xylanase gene (xynA) from the rumen fungus, Neocallimastix patriciarum, linked with an endosperm-specific promoter from cereal storage protein genes was introduced into barley by Agrobacterium-mediated transformation. Twenty-four independently transformed barley lines with the xylanase gene were produced and analysed. The fungal xylanase was produced in the developing endosperm under the control of either the rice glutelin B-1 (GluB-1) or barley B1 hordein (Hor2-4) promoter. The rice GluB-1 promoter provided an apparently higher expression level of recombinant proteins in barley grain than the barley Hor2-4 promoter in both transient and stable expression experiments. In particular, the mean value for the fungal xylanase activity driven by the GluB-1 promoter in the mature grains of transgenic barley was more than twice that with the Hor2-4 promoter. Expression of the xylanase transgene under these endosperm-specific promoters was not observed in the leaf, stem and root tissues. Accumulation of the fungal xylanase in the developing grains of transgenic barley followed the pattern of storage protein deposition. The xylanase was stably maintained in the grain during grain maturation and desiccation and post-harvest storage. These results indicate that the cereal grain expression system may provide an economic means for large scale production of feed enzymes in the future. More... »

PAGES

113-124

Journal

TITLE

Molecular Breeding

ISSUE

1

VOLUME

6

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1009640427515

DOI

http://dx.doi.org/10.1023/a:1009640427515

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1036776454


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "name": [
            "CSIRO Tropical Agriculture, 306 Carmody Rd., 4067, St. Lucia, QLD, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Patel", 
        "givenName": "Minesh", 
        "id": "sg:person.01216540222.08", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01216540222.08"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "CSIRO Tropical Agriculture, 306 Carmody Rd., 4067, St. Lucia, QLD, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Johnson", 
        "givenName": "Jennifer S.", 
        "id": "sg:person.01076377234.31", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01076377234.31"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Plant Industry", 
          "id": "https://www.grid.ac/institutes/grid.417667.5", 
          "name": [
            "CSIRO Plant Industry, GPO Box 1600, 2601, Canberra, ACT, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Brettell", 
        "givenName": "Richard I.S.", 
        "id": "sg:person.013014502564.15", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013014502564.15"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Plant Industry", 
          "id": "https://www.grid.ac/institutes/grid.417667.5", 
          "name": [
            "CSIRO Plant Industry, GPO Box 1600, 2601, Canberra, ACT, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jacobsen", 
        "givenName": "Jake", 
        "id": "sg:person.016441017673.24", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016441017673.24"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "CSIRO Tropical Agriculture, 306 Carmody Rd., 4067, St. Lucia, QLD, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Xue", 
        "givenName": "Gang-Ping", 
        "id": "sg:person.0705112730.93", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0705112730.93"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf02907156", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000868111", 
          "https://doi.org/10.1007/bf02907156"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02907156", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000868111", 
          "https://doi.org/10.1007/bf02907156"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00017916", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001099103", 
          "https://doi.org/10.1007/bf00017916"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00017916", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001099103", 
          "https://doi.org/10.1007/bf00017916"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1079/bjn19890014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003851682"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1079/bjn19890014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003851682"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00037068", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008140582", 
          "https://doi.org/10.1007/bf00037068"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00037068", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008140582", 
          "https://doi.org/10.1007/bf00037068"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-313x.1997.11061369.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009597905"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt1091-963", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012182392", 
          "https://doi.org/10.1038/nbt1091-963"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0377-8401(95)02018-u", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012555100"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0167-7799(00)88977-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013446262"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02667740", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017993505", 
          "https://doi.org/10.1007/bf02667740"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02667740", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017993505", 
          "https://doi.org/10.1007/bf02667740"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-94-015-8909-3_3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018658009", 
          "https://doi.org/10.1007/978-94-015-8909-3_3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02907181", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021325688", 
          "https://doi.org/10.1007/bf02907181"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02907181", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021325688", 
          "https://doi.org/10.1007/bf02907181"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0168-9452(95)04215-g", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022578374"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00040660", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023698763", 
          "https://doi.org/10.1007/bf00040660"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00040660", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023698763", 
          "https://doi.org/10.1007/bf00040660"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00201052", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023864032", 
          "https://doi.org/10.1007/bf00201052"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00201052", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023864032", 
          "https://doi.org/10.1007/bf00201052"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/17.20.8385", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028859512"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01690268", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029082760", 
          "https://doi.org/10.1007/bf01690268"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01690268", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029082760", 
          "https://doi.org/10.1007/bf01690268"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01690268", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029082760", 
          "https://doi.org/10.1007/bf01690268"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00171354", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029896329", 
          "https://doi.org/10.1007/bf00171354"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00171354", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029896329", 
          "https://doi.org/10.1007/bf00171354"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1071/pp97095", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031175638"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0195-63", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033427699", 
          "https://doi.org/10.1038/nbt0195-63"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00039026", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039114428", 
          "https://doi.org/10.1007/bf00039026"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00039026", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039114428", 
          "https://doi.org/10.1007/bf00039026"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/00071669008417312", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039299521"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1009604119618", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040251208", 
          "https://doi.org/10.1023/a:1009604119618"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s001220050758", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040346801", 
          "https://doi.org/10.1007/s001220050758"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2958.1992.tb01379.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043177031"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0168-1656(94)00133-w", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043574818"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/13.20.7327", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044153838"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00041391", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045495849", 
          "https://doi.org/10.1007/bf00041391"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00041391", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045495849", 
          "https://doi.org/10.1007/bf00041391"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00233358", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045705949", 
          "https://doi.org/10.1007/bf00233358"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00233358", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045705949", 
          "https://doi.org/10.1007/bf00233358"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jsfa.2740680102", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052267116"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/00221287-138-7-1413", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060368424"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1104/pp.107.3.679", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060840650"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082546596", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082955266", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2000-02", 
    "datePublishedReg": "2000-02-01", 
    "description": "The feasibility of producing plant cell wall polysaccharide-hydrolysing feed enzymes in the endosperm of barley grain was investigated. The coding region of a modified xylanase gene (xynA) from the rumen fungus, Neocallimastix patriciarum, linked with an endosperm-specific promoter from cereal storage protein genes was introduced into barley by Agrobacterium-mediated transformation. Twenty-four independently transformed barley lines with the xylanase gene were produced and analysed. The fungal xylanase was produced in the developing endosperm under the control of either the rice glutelin B-1 (GluB-1) or barley B1 hordein (Hor2-4) promoter. The rice GluB-1 promoter provided an apparently higher expression level of recombinant proteins in barley grain than the barley Hor2-4 promoter in both transient and stable expression experiments. In particular, the mean value for the fungal xylanase activity driven by the GluB-1 promoter in the mature grains of transgenic barley was more than twice that with the Hor2-4 promoter. Expression of the xylanase transgene under these endosperm-specific promoters was not observed in the leaf, stem and root tissues. Accumulation of the fungal xylanase in the developing grains of transgenic barley followed the pattern of storage protein deposition. The xylanase was stably maintained in the grain during grain maturation and desiccation and post-harvest storage. These results indicate that the cereal grain expression system may provide an economic means for large scale production of feed enzymes in the future.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1023/a:1009640427515", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1114043", 
        "issn": [
          "1380-3743", 
          "1572-9788"
        ], 
        "name": "Molecular Breeding", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "6"
      }
    ], 
    "name": "Transgenic barley expressing a fungal xylanase gene in the endosperm of the developing grains", 
    "pagination": "113-124", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "af307b9e24c61d56bd998758d474ef85f4a1d7a75a6e82ce96319d70a29a5d85"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1023/a:1009640427515"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1036776454"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1023/a:1009640427515", 
      "https://app.dimensions.ai/details/publication/pub.1036776454"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T20:44", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8684_00000500.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1023/A:1009640427515"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1023/a:1009640427515'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1023/a:1009640427515'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1023/a:1009640427515'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1023/a:1009640427515'


 

This table displays all metadata directly associated to this object as RDF triples.

209 TRIPLES      21 PREDICATES      60 URIs      19 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1023/a:1009640427515 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N66b41e5923824ea1924ead4f59d28796
4 schema:citation sg:pub.10.1007/978-94-015-8909-3_3
5 sg:pub.10.1007/bf00017916
6 sg:pub.10.1007/bf00037068
7 sg:pub.10.1007/bf00039026
8 sg:pub.10.1007/bf00040660
9 sg:pub.10.1007/bf00041391
10 sg:pub.10.1007/bf00171354
11 sg:pub.10.1007/bf00201052
12 sg:pub.10.1007/bf00233358
13 sg:pub.10.1007/bf01690268
14 sg:pub.10.1007/bf02667740
15 sg:pub.10.1007/bf02907156
16 sg:pub.10.1007/bf02907181
17 sg:pub.10.1007/s001220050758
18 sg:pub.10.1023/a:1009604119618
19 sg:pub.10.1038/nbt0195-63
20 sg:pub.10.1038/nbt1091-963
21 https://app.dimensions.ai/details/publication/pub.1082546596
22 https://app.dimensions.ai/details/publication/pub.1082955266
23 https://doi.org/10.1002/jsfa.2740680102
24 https://doi.org/10.1016/0168-1656(94)00133-w
25 https://doi.org/10.1016/0168-9452(95)04215-g
26 https://doi.org/10.1016/0377-8401(95)02018-u
27 https://doi.org/10.1016/s0167-7799(00)88977-5
28 https://doi.org/10.1046/j.1365-313x.1997.11061369.x
29 https://doi.org/10.1071/pp97095
30 https://doi.org/10.1079/bjn19890014
31 https://doi.org/10.1080/00071669008417312
32 https://doi.org/10.1093/nar/13.20.7327
33 https://doi.org/10.1093/nar/17.20.8385
34 https://doi.org/10.1099/00221287-138-7-1413
35 https://doi.org/10.1104/pp.107.3.679
36 https://doi.org/10.1111/j.1365-2958.1992.tb01379.x
37 schema:datePublished 2000-02
38 schema:datePublishedReg 2000-02-01
39 schema:description The feasibility of producing plant cell wall polysaccharide-hydrolysing feed enzymes in the endosperm of barley grain was investigated. The coding region of a modified xylanase gene (xynA) from the rumen fungus, Neocallimastix patriciarum, linked with an endosperm-specific promoter from cereal storage protein genes was introduced into barley by Agrobacterium-mediated transformation. Twenty-four independently transformed barley lines with the xylanase gene were produced and analysed. The fungal xylanase was produced in the developing endosperm under the control of either the rice glutelin B-1 (GluB-1) or barley B1 hordein (Hor2-4) promoter. The rice GluB-1 promoter provided an apparently higher expression level of recombinant proteins in barley grain than the barley Hor2-4 promoter in both transient and stable expression experiments. In particular, the mean value for the fungal xylanase activity driven by the GluB-1 promoter in the mature grains of transgenic barley was more than twice that with the Hor2-4 promoter. Expression of the xylanase transgene under these endosperm-specific promoters was not observed in the leaf, stem and root tissues. Accumulation of the fungal xylanase in the developing grains of transgenic barley followed the pattern of storage protein deposition. The xylanase was stably maintained in the grain during grain maturation and desiccation and post-harvest storage. These results indicate that the cereal grain expression system may provide an economic means for large scale production of feed enzymes in the future.
40 schema:genre research_article
41 schema:inLanguage en
42 schema:isAccessibleForFree false
43 schema:isPartOf N4a13c47b3fef455597c0ec6357c8fc1c
44 N60b68c327a344011a6102dc9c5d98918
45 sg:journal.1114043
46 schema:name Transgenic barley expressing a fungal xylanase gene in the endosperm of the developing grains
47 schema:pagination 113-124
48 schema:productId N407b6cda682a4637a03370292932707b
49 N8e443495c1884a929cb916d826611393
50 Nbb61fb41e7854e3bb463876ae02358fb
51 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036776454
52 https://doi.org/10.1023/a:1009640427515
53 schema:sdDatePublished 2019-04-10T20:44
54 schema:sdLicense https://scigraph.springernature.com/explorer/license/
55 schema:sdPublisher N679936ff4d6043beb3a662512b95f9fb
56 schema:url http://link.springer.com/10.1023/A:1009640427515
57 sgo:license sg:explorer/license/
58 sgo:sdDataset articles
59 rdf:type schema:ScholarlyArticle
60 N07e8e9406bb54eefa117254660bf3c43 rdf:first sg:person.016441017673.24
61 rdf:rest Nde2ef8a7e848421babe1e27b75609cbd
62 N1e31da105b834dee9fc1e5a86a1cb98a schema:name CSIRO Tropical Agriculture, 306 Carmody Rd., 4067, St. Lucia, QLD, Australia
63 rdf:type schema:Organization
64 N2da6933ae73145b8acf83d4d9fd95e9b schema:name CSIRO Tropical Agriculture, 306 Carmody Rd., 4067, St. Lucia, QLD, Australia
65 rdf:type schema:Organization
66 N407b6cda682a4637a03370292932707b schema:name readcube_id
67 schema:value af307b9e24c61d56bd998758d474ef85f4a1d7a75a6e82ce96319d70a29a5d85
68 rdf:type schema:PropertyValue
69 N4a13c47b3fef455597c0ec6357c8fc1c schema:issueNumber 1
70 rdf:type schema:PublicationIssue
71 N60b68c327a344011a6102dc9c5d98918 schema:volumeNumber 6
72 rdf:type schema:PublicationVolume
73 N66b41e5923824ea1924ead4f59d28796 rdf:first sg:person.01216540222.08
74 rdf:rest Naa960074d5714e3ebb73923a2c2d7ff7
75 N679936ff4d6043beb3a662512b95f9fb schema:name Springer Nature - SN SciGraph project
76 rdf:type schema:Organization
77 N8e443495c1884a929cb916d826611393 schema:name doi
78 schema:value 10.1023/a:1009640427515
79 rdf:type schema:PropertyValue
80 Naa960074d5714e3ebb73923a2c2d7ff7 rdf:first sg:person.01076377234.31
81 rdf:rest Ncbbd54cd74844acdb7a419fd0aef20a3
82 Nbb61fb41e7854e3bb463876ae02358fb schema:name dimensions_id
83 schema:value pub.1036776454
84 rdf:type schema:PropertyValue
85 Ncbbd54cd74844acdb7a419fd0aef20a3 rdf:first sg:person.013014502564.15
86 rdf:rest N07e8e9406bb54eefa117254660bf3c43
87 Nde2ef8a7e848421babe1e27b75609cbd rdf:first sg:person.0705112730.93
88 rdf:rest rdf:nil
89 Ne6040c25ab564c828f178b2d8fbf24a7 schema:name CSIRO Tropical Agriculture, 306 Carmody Rd., 4067, St. Lucia, QLD, Australia
90 rdf:type schema:Organization
91 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
92 schema:name Biological Sciences
93 rdf:type schema:DefinedTerm
94 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
95 schema:name Biochemistry and Cell Biology
96 rdf:type schema:DefinedTerm
97 sg:journal.1114043 schema:issn 1380-3743
98 1572-9788
99 schema:name Molecular Breeding
100 rdf:type schema:Periodical
101 sg:person.01076377234.31 schema:affiliation Ne6040c25ab564c828f178b2d8fbf24a7
102 schema:familyName Johnson
103 schema:givenName Jennifer S.
104 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01076377234.31
105 rdf:type schema:Person
106 sg:person.01216540222.08 schema:affiliation N1e31da105b834dee9fc1e5a86a1cb98a
107 schema:familyName Patel
108 schema:givenName Minesh
109 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01216540222.08
110 rdf:type schema:Person
111 sg:person.013014502564.15 schema:affiliation https://www.grid.ac/institutes/grid.417667.5
112 schema:familyName Brettell
113 schema:givenName Richard I.S.
114 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013014502564.15
115 rdf:type schema:Person
116 sg:person.016441017673.24 schema:affiliation https://www.grid.ac/institutes/grid.417667.5
117 schema:familyName Jacobsen
118 schema:givenName Jake
119 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016441017673.24
120 rdf:type schema:Person
121 sg:person.0705112730.93 schema:affiliation N2da6933ae73145b8acf83d4d9fd95e9b
122 schema:familyName Xue
123 schema:givenName Gang-Ping
124 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0705112730.93
125 rdf:type schema:Person
126 sg:pub.10.1007/978-94-015-8909-3_3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018658009
127 https://doi.org/10.1007/978-94-015-8909-3_3
128 rdf:type schema:CreativeWork
129 sg:pub.10.1007/bf00017916 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001099103
130 https://doi.org/10.1007/bf00017916
131 rdf:type schema:CreativeWork
132 sg:pub.10.1007/bf00037068 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008140582
133 https://doi.org/10.1007/bf00037068
134 rdf:type schema:CreativeWork
135 sg:pub.10.1007/bf00039026 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039114428
136 https://doi.org/10.1007/bf00039026
137 rdf:type schema:CreativeWork
138 sg:pub.10.1007/bf00040660 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023698763
139 https://doi.org/10.1007/bf00040660
140 rdf:type schema:CreativeWork
141 sg:pub.10.1007/bf00041391 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045495849
142 https://doi.org/10.1007/bf00041391
143 rdf:type schema:CreativeWork
144 sg:pub.10.1007/bf00171354 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029896329
145 https://doi.org/10.1007/bf00171354
146 rdf:type schema:CreativeWork
147 sg:pub.10.1007/bf00201052 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023864032
148 https://doi.org/10.1007/bf00201052
149 rdf:type schema:CreativeWork
150 sg:pub.10.1007/bf00233358 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045705949
151 https://doi.org/10.1007/bf00233358
152 rdf:type schema:CreativeWork
153 sg:pub.10.1007/bf01690268 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029082760
154 https://doi.org/10.1007/bf01690268
155 rdf:type schema:CreativeWork
156 sg:pub.10.1007/bf02667740 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017993505
157 https://doi.org/10.1007/bf02667740
158 rdf:type schema:CreativeWork
159 sg:pub.10.1007/bf02907156 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000868111
160 https://doi.org/10.1007/bf02907156
161 rdf:type schema:CreativeWork
162 sg:pub.10.1007/bf02907181 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021325688
163 https://doi.org/10.1007/bf02907181
164 rdf:type schema:CreativeWork
165 sg:pub.10.1007/s001220050758 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040346801
166 https://doi.org/10.1007/s001220050758
167 rdf:type schema:CreativeWork
168 sg:pub.10.1023/a:1009604119618 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040251208
169 https://doi.org/10.1023/a:1009604119618
170 rdf:type schema:CreativeWork
171 sg:pub.10.1038/nbt0195-63 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033427699
172 https://doi.org/10.1038/nbt0195-63
173 rdf:type schema:CreativeWork
174 sg:pub.10.1038/nbt1091-963 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012182392
175 https://doi.org/10.1038/nbt1091-963
176 rdf:type schema:CreativeWork
177 https://app.dimensions.ai/details/publication/pub.1082546596 schema:CreativeWork
178 https://app.dimensions.ai/details/publication/pub.1082955266 schema:CreativeWork
179 https://doi.org/10.1002/jsfa.2740680102 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052267116
180 rdf:type schema:CreativeWork
181 https://doi.org/10.1016/0168-1656(94)00133-w schema:sameAs https://app.dimensions.ai/details/publication/pub.1043574818
182 rdf:type schema:CreativeWork
183 https://doi.org/10.1016/0168-9452(95)04215-g schema:sameAs https://app.dimensions.ai/details/publication/pub.1022578374
184 rdf:type schema:CreativeWork
185 https://doi.org/10.1016/0377-8401(95)02018-u schema:sameAs https://app.dimensions.ai/details/publication/pub.1012555100
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1016/s0167-7799(00)88977-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013446262
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1046/j.1365-313x.1997.11061369.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1009597905
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1071/pp97095 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031175638
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1079/bjn19890014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003851682
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1080/00071669008417312 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039299521
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1093/nar/13.20.7327 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044153838
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1093/nar/17.20.8385 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028859512
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1099/00221287-138-7-1413 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060368424
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1104/pp.107.3.679 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060840650
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1111/j.1365-2958.1992.tb01379.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1043177031
206 rdf:type schema:CreativeWork
207 https://www.grid.ac/institutes/grid.417667.5 schema:alternateName Plant Industry
208 schema:name CSIRO Plant Industry, GPO Box 1600, 2601, Canberra, ACT, Australia
209 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...