Transgenic plants expressing human glutamic acid decarboxylase (GAD65), a major autoantigen in insulin-dependent diabetes mellitus View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1999-12

AUTHORS

Andrea Porceddu, Alberto Falorni, Nicoletta Ferradini, Anna Cosentino, Filippo Calcinaro, Claudia Faleri, Mauro Cresti, Franco Lorenzetti, Paolo Brunetti, Mario Pezzotti

ABSTRACT

Parenteral and oral administration of autoantigens can induce immune tolerance in autoimmune diseases. Prophylactic therapy based on oral administration of human autoantigens is not, however, feasible when sufficient quantities of candidate autoantigens are not available. Transgenic plants that express high levels of recombinant proteins would allow large quantities of autoantigens to be produced at relatively low costs. In addition, transgenic food would provide a simple and direct method of delivering autoantigens. The production and the characterization of transgenic tobacco and carrot plants expressing human GAD65, a major autoantigen in human insulin-dependent diabetes mellitus (IDDM), is reported. Immunogold labeling and electron microscopy of transgenic tobacco tissue shows the selective targeting of human GAD65 to chloroplast tylacoids and mitochondria. In planta expressed GAD65 has a correct immunoreactivity with IDDM-associated autoantibodies and retains enzymatic activity, a finding that suggests a correct protein folding. In transgenic tobacco and carrot the expression levels of human GAD65 varies between 0.01% and 0.04% of total soluble proteins. Transgenic edible plant organs are now available to study the feasibility of inducing immune tolerance in IDDM animals by oral administration of GAD65. More... »

PAGES

553-560

References to SciGraph publications

  • 1997-02. T-cell reactivity to GAD65 peptide sequences shared with coxsackie virus protein in recent-onset IDDM, post-onset IDDM patients and control subjects in DIABETOLOGIA
  • 1997-07. Expression of the human milk protein β-casein in transgenic potato plants in TRANSGENIC RESEARCH
  • 1998-10. A plant-based cholera toxin B subunit–insulin fusion protein protects against the development of autoimmune diabetes in NATURE BIOTECHNOLOGY
  • 1995-03. Immunogold localization of arabinogalactan proteins, unesterified and esterified pectins in pollen grains and pollen tubes ofNicotiana tabacum L. in PROTOPLASMA
  • 1998-08. Two isoforms of glutamate decarboxylase in Arabidopsis are regulated by calcium/calmodulin and differ in organ distribution in PLANT MOLECULAR BIOLOGY
  • 1997-07. Transgenic plants expressing autoantigens fed to mice to induce oral immune tolerance in NATURE MEDICINE
  • 1993-11. Immune response to glutamic acid decarboxylase correlates with insulitis in non-obese diabetic mice in NATURE
  • 1997-03. Human haemoglobin from transgenic tobacco in NATURE
  • 1996-09. Diagnostic sensitivity of immunodominant epitopes of glutamic acid decarboxylase (GAD65) autoantibodies in childhood IDDM in DIABETOLOGIA
  • 1993-11. Spontaneous loss of T-cell tolerance to glutamic acid decarboxylase in murine insulin-dependent diabetes in NATURE
  • 1994-04. A novel radioligand binding assay to determine diagnostic accuracy of isoform-specific glutamic acid decarboxylase antibodies in childhood IDDM in DIABETOLOGIA
  • 1989-06. Carrot (Daucus carota) hypocotyl transformation usingAgrobacterium tumefaciens in PLANT CELL REPORTS
  • 1998-05. Immunogenicity in humans of a recombinant bacterial antigen delivered in a transgenic potato in NATURE MEDICINE
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    http://scigraph.springernature.com/pub.10.1023/a:1009605729268

    DOI

    http://dx.doi.org/10.1023/a:1009605729268

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1010236354


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