Organization of Repetitive DNA Sequences at Pachytene Chromosomes of Gilthead Seabream Sparus aurata (Pisces, Perciformes) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-01

AUTHORS

N. Cuñado, M. A. Garrido-Ramos, R. de la Herrán, C. Ruíz Rejón, M. Ruíz Rejón, J. L. Santos

ABSTRACT

A method of preparing two-dimensional surface spreads of fish synaptonemal complexes (SCs) associated with fluorescent in-situ hybridization is described. This technique permits a novel approach to the analysis of chromatin organization and the construction of physical maps at meiosis, since surface-spread pachytene chromosomes are several times the length of metaphase chromosomes and the decondensed chromatin loops are attached to the lateral elements of the SC. We have applied this technique to analyze the location and organization of three different repetitive DNA sequences, rDNA, an EcoRI satellite DNA of the Sparidae family and telomere DNA in the gilthead seabream Sparus aurata. Our observations indicate that, depending on the type of sequence, the chromatin has different properties with regard to anchorage to the SC. More... »

PAGES

67-72

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1009283321632

DOI

http://dx.doi.org/10.1023/a:1009283321632

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1040408812

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/10730590


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