Separate and variably shaped chromosome arm domains are disclosed by chromosome arm painting in human cell nuclei View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-01

AUTHORS

Steffen Dietzel, Anna Jauch, Dirk Kienle, Guoquiong Qu, Heidi Holtgreve-Grez, Roland Eils, Christian Munkel, Michael Bittner, Paul S. Meltzer, Jeffrey M. Trent, Thomas Cremer

ABSTRACT

Fluorescence in situ hybridization (FISH) with microdissection probes from human chromosomes 3 and 6 was applied to visualize arm and subregional band domains in human amniotic fluid cell nuclei. Confocal laser scanning microscopy and quantitative three-dimensional image analysis showed a pronounced variability of p- and q-arm domain arrangements and shapes. Apparent intermingling of neighbouring arm domains was limited to the domain surface. Three-dimensional distance measurements with pter and qter probes supported a high variability of chromosome territory folding. More... »

PAGES

25-33

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1009262223693

DOI

http://dx.doi.org/10.1023/a:1009262223693

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1010175929

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/9510507


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