Development of a new transformation method using magnetite cationic liposomes and magnetic selection of transformed cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-07

AUTHORS

N. Nagatani, M. Shinkai, H. Honda, T. Kobayashi

ABSTRACT

A new method using magnetite cationic liposomes (MCLs)/DNA complex for transformation is proposed and efficient separation of transformed cells is reported. In our method, gene expression and antibodies as a marker are not required for separation. The specific activity of separated cells was the highest when the complex consisting of 25 μg MCLs and 2 μg plasmid DNA was used for transformation. The specific activity was about 5 times higher than that of the cells before the separation. © Rapid Science Ltd. 1998 More... »

PAGES

525-528

Journal

TITLE

Biotechnology Letters

ISSUE

7

VOLUME

12

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1008899313722

DOI

http://dx.doi.org/10.1023/a:1008899313722

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1019994851


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