Recovery of β-Galactosidase from E. Coli Double Lysogen Using Aqueous Two Phase System View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-06

AUTHORS

Woo-Jin Chang, Yoon-Mo Koo

ABSTRACT

An aqueous two phase system (ATPS) was employed to recover β-galactosidase from the lytic broth of the phage double lysogen system, where production of an intracellular product and cell disruption are carried out sequentially in a container. Partition of β-galactosidase was more favorable to PEG phase comparing to total proteins, which was enhanced with Na2SO4. Recovery of β-galactosidase was more than 90% in most cases, which was much more the recovery of total proteins. More... »

PAGES

455-457

References to SciGraph publications

Journal

TITLE

Biotechnology Letters

ISSUE

6

VOLUME

12

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1008807322151

DOI

http://dx.doi.org/10.1023/a:1008807322151

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1046646005


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Inha University", 
          "id": "https://www.grid.ac/institutes/grid.202119.9", 
          "name": [
            "Department of Biological Engineering, Inha University 253 Yonghyungdong, 402-751, Inchon, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chang", 
        "givenName": "Woo-Jin", 
        "id": "sg:person.01233213336.41", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01233213336.41"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Inha University", 
          "id": "https://www.grid.ac/institutes/grid.202119.9", 
          "name": [
            "Department of Biological Engineering, Inha University 253 Yonghyungdong, 402-751, Inchon, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Koo", 
        "givenName": "Yoon-Mo", 
        "id": "sg:person.01313270030.22", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01313270030.22"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/0141-0229(84)90062-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001280349"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0141-0229(84)90062-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001280349"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01022393", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013480698", 
          "https://doi.org/10.1007/bf01022393"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bp00032a012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022010439"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1998-06", 
    "datePublishedReg": "1998-06-01", 
    "description": "An aqueous two phase system (ATPS) was employed to recover \u03b2-galactosidase from the lytic broth of the phage double lysogen system, where production of an intracellular product and cell disruption are carried out sequentially in a container. Partition of \u03b2-galactosidase was more favorable to PEG phase comparing to total proteins, which was enhanced with Na2SO4. Recovery of \u03b2-galactosidase was more than 90% in most cases, which was much more the recovery of total proteins.", 
    "genre": "non_research_article", 
    "id": "sg:pub.10.1023/a:1008807322151", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1289037", 
        "issn": [
          "0951-208X", 
          "1573-6784"
        ], 
        "name": "Biotechnology Letters", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "12"
      }
    ], 
    "name": "Recovery of \u03b2-Galactosidase from E. Coli Double Lysogen Using Aqueous Two Phase System", 
    "pagination": "455-457", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "de33cbdf1955144b61db6c59c967ff7d4a140b5d82ff65abe9337af069f2af41"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1023/a:1008807322151"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1046646005"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1023/a:1008807322151", 
      "https://app.dimensions.ai/details/publication/pub.1046646005"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T23:22", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8693_00000501.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1023/A:1008807322151"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1023/a:1008807322151'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1023/a:1008807322151'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1023/a:1008807322151'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1023/a:1008807322151'


 

This table displays all metadata directly associated to this object as RDF triples.

78 TRIPLES      21 PREDICATES      30 URIs      19 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1023/a:1008807322151 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N440a4a8fa7e94047ab5b406db5b37ba2
4 schema:citation sg:pub.10.1007/bf01022393
5 https://doi.org/10.1016/0141-0229(84)90062-0
6 https://doi.org/10.1021/bp00032a012
7 schema:datePublished 1998-06
8 schema:datePublishedReg 1998-06-01
9 schema:description An aqueous two phase system (ATPS) was employed to recover β-galactosidase from the lytic broth of the phage double lysogen system, where production of an intracellular product and cell disruption are carried out sequentially in a container. Partition of β-galactosidase was more favorable to PEG phase comparing to total proteins, which was enhanced with Na2SO4. Recovery of β-galactosidase was more than 90% in most cases, which was much more the recovery of total proteins.
10 schema:genre non_research_article
11 schema:inLanguage en
12 schema:isAccessibleForFree false
13 schema:isPartOf N25f9592a80b74bbb830d2ab8038335d0
14 Nbc85c92c9c604530b6a1e3e9b3218243
15 sg:journal.1289037
16 schema:name Recovery of β-Galactosidase from E. Coli Double Lysogen Using Aqueous Two Phase System
17 schema:pagination 455-457
18 schema:productId N68581ef57c024ad3b671a2bd5d03c36c
19 N7e9b41fe5e394ca896b065a1c59e6384
20 N8d433a5cfb34444ba285ac475ccc9778
21 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046646005
22 https://doi.org/10.1023/a:1008807322151
23 schema:sdDatePublished 2019-04-10T23:22
24 schema:sdLicense https://scigraph.springernature.com/explorer/license/
25 schema:sdPublisher N937080431ab44e63a58a3f9cd647af8f
26 schema:url http://link.springer.com/10.1023/A:1008807322151
27 sgo:license sg:explorer/license/
28 sgo:sdDataset articles
29 rdf:type schema:ScholarlyArticle
30 N25f9592a80b74bbb830d2ab8038335d0 schema:volumeNumber 12
31 rdf:type schema:PublicationVolume
32 N440a4a8fa7e94047ab5b406db5b37ba2 rdf:first sg:person.01233213336.41
33 rdf:rest N468f211efe574d4b8f07caeb17e95358
34 N468f211efe574d4b8f07caeb17e95358 rdf:first sg:person.01313270030.22
35 rdf:rest rdf:nil
36 N68581ef57c024ad3b671a2bd5d03c36c schema:name doi
37 schema:value 10.1023/a:1008807322151
38 rdf:type schema:PropertyValue
39 N7e9b41fe5e394ca896b065a1c59e6384 schema:name readcube_id
40 schema:value de33cbdf1955144b61db6c59c967ff7d4a140b5d82ff65abe9337af069f2af41
41 rdf:type schema:PropertyValue
42 N8d433a5cfb34444ba285ac475ccc9778 schema:name dimensions_id
43 schema:value pub.1046646005
44 rdf:type schema:PropertyValue
45 N937080431ab44e63a58a3f9cd647af8f schema:name Springer Nature - SN SciGraph project
46 rdf:type schema:Organization
47 Nbc85c92c9c604530b6a1e3e9b3218243 schema:issueNumber 6
48 rdf:type schema:PublicationIssue
49 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
50 schema:name Biological Sciences
51 rdf:type schema:DefinedTerm
52 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
53 schema:name Biochemistry and Cell Biology
54 rdf:type schema:DefinedTerm
55 sg:journal.1289037 schema:issn 0951-208X
56 1573-6784
57 schema:name Biotechnology Letters
58 rdf:type schema:Periodical
59 sg:person.01233213336.41 schema:affiliation https://www.grid.ac/institutes/grid.202119.9
60 schema:familyName Chang
61 schema:givenName Woo-Jin
62 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01233213336.41
63 rdf:type schema:Person
64 sg:person.01313270030.22 schema:affiliation https://www.grid.ac/institutes/grid.202119.9
65 schema:familyName Koo
66 schema:givenName Yoon-Mo
67 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01313270030.22
68 rdf:type schema:Person
69 sg:pub.10.1007/bf01022393 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013480698
70 https://doi.org/10.1007/bf01022393
71 rdf:type schema:CreativeWork
72 https://doi.org/10.1016/0141-0229(84)90062-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001280349
73 rdf:type schema:CreativeWork
74 https://doi.org/10.1021/bp00032a012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022010439
75 rdf:type schema:CreativeWork
76 https://www.grid.ac/institutes/grid.202119.9 schema:alternateName Inha University
77 schema:name Department of Biological Engineering, Inha University 253 Yonghyungdong, 402-751, Inchon, Korea
78 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...