Sol-Gel Immobilized Glucose-Oxidase: Calorimetric Investigations of Enzyme Activities View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-01

AUTHORS

U. Georgi, H. Graebner, G. Roewer, G. Wolf

ABSTRACT

Using the sol-gel process, the enzyme glucose-oxidase was immobilized in modified SiO2 layers. The tetraethoxysilane based sol was varied by veratrylaldehyde, aminopropyltriethoxysilane and an azomethine compound, which was synthesized from both molecules. Changes in reaction kinetics of the sol-gel process by addition of veratrylaldehyde were observed by 29Si NMR measurements. The fixation of this additive in the final gel was clarified by FTIR spectroscopy.The influence of inserted functional groups on the enzyme activity was determined by calorimetry. The Enzyme Thermistor is a fast and uncomplicated method to obtain comparative results of sol-gel immobilized enzyme activity. Differences in immobilization effects were found dependent on structure and concentration of additives. More... »

PAGES

295-298

References to SciGraph publications

  • 1982-01. The enzyme thermistor in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 1994-01. Thin films of functionalized amorphous silica for immunosensors application in JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1023/a:1008611924361

    DOI

    http://dx.doi.org/10.1023/a:1008611924361

    DIMENSIONS

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