Development of a fixed bed bioreactor for the expansion of human hematopoietic progenitor cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1999-07

AUTHORS

Petra Meissner, Bernd Schröder, Cornelia Herfurth, Manfred Biselli

ABSTRACT

The ex vivo expansion of hematopoietic progenitor cells is of great interest for a variety of clinical applications, e.g. bone marrow transplantation or gene therapy. Therefore it is of general interest to develop a culture system, able to mimic the in vivo hematopoesis, which is a prerequisite for long-term hematopoietic culture. Our approach was to modify a continuously perfused bioreactor for cultivation and expansion of human hematopoietic stem cells. Therefore we immobilized stromal cells (human primary stromal cells or the murine cell line M2-10B4) in porous glass carriers in a fixed bed reactor and cocultivated human hematopoietic progenitor cells for several weeks. After inoculation of mononuclear cells derived from umbilical cord blood or peripheral blood stem cells both adherent and non adherent cells were harvested and analyzed by flow cytometry and short-term colony assays. During cultivation there was a permanent production of progenitor cells and mature blood cells derived from the immobilized cells in the carriers. We could demonstrate the immobilization of hematopoietic progenitor cells of the myeloid system detectable in short-term colony assays. Additionally we could observe the expansion of very early progenitor cells (CFU-GEMM) up to 4.2-fold and later progenitor cells (CFU-GM and BFU-E) up to 7-fold and 1.8-fold, respectively. More... »

PAGES

227-234

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1008085932764

DOI

http://dx.doi.org/10.1023/a:1008085932764

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1020997857

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19003372


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