Development of a fixed bed bioreactor for the expansion of human hematopoietic progenitor cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1999-07

AUTHORS

Petra Meissner, Bernd Schröder, Cornelia Herfurth, Manfred Biselli

ABSTRACT

The ex vivo expansion of hematopoietic progenitor cells is of great interest for a variety of clinical applications, e.g. bone marrow transplantation or gene therapy. Therefore it is of general interest to develop a culture system, able to mimic the in vivo hematopoesis, which is a prerequisite for long-term hematopoietic culture. Our approach was to modify a continuously perfused bioreactor for cultivation and expansion of human hematopoietic stem cells. Therefore we immobilized stromal cells (human primary stromal cells or the murine cell line M2-10B4) in porous glass carriers in a fixed bed reactor and cocultivated human hematopoietic progenitor cells for several weeks. After inoculation of mononuclear cells derived from umbilical cord blood or peripheral blood stem cells both adherent and non adherent cells were harvested and analyzed by flow cytometry and short-term colony assays. During cultivation there was a permanent production of progenitor cells and mature blood cells derived from the immobilized cells in the carriers. We could demonstrate the immobilization of hematopoietic progenitor cells of the myeloid system detectable in short-term colony assays. Additionally we could observe the expansion of very early progenitor cells (CFU-GEMM) up to 4.2-fold and later progenitor cells (CFU-GM and BFU-E) up to 7-fold and 1.8-fold, respectively. More... »

PAGES

227-234

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1008085932764

DOI

http://dx.doi.org/10.1023/a:1008085932764

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1020997857

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19003372


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1102", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Cardiorespiratory Medicine and Haematology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Forschungszentrum J\u00fclich", 
          "id": "https://www.grid.ac/institutes/grid.8385.6", 
          "name": [
            "Institute of Biotechnology 2, Forschungszentrum J\u00fclich, 52425, J\u00fclich GmbH, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Meissner", 
        "givenName": "Petra", 
        "id": "sg:person.01325210552.08", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01325210552.08"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Forschungszentrum J\u00fclich", 
          "id": "https://www.grid.ac/institutes/grid.8385.6", 
          "name": [
            "Institute of Biotechnology 2, Forschungszentrum J\u00fclich, 52425, J\u00fclich GmbH, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Schr\u00f6der", 
        "givenName": "Bernd", 
        "id": "sg:person.0601604452.95", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0601604452.95"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Forschungszentrum J\u00fclich", 
          "id": "https://www.grid.ac/institutes/grid.8385.6", 
          "name": [
            "Institute of Biotechnology 2, Forschungszentrum J\u00fclich, 52425, J\u00fclich GmbH, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Herfurth", 
        "givenName": "Cornelia", 
        "id": "sg:person.0647717652.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0647717652.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Forschungszentrum J\u00fclich", 
          "id": "https://www.grid.ac/institutes/grid.8385.6", 
          "name": [
            "Institute of Biotechnology 2, Forschungszentrum J\u00fclich, 52425, J\u00fclich GmbH, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Biselli", 
        "givenName": "Manfred", 
        "id": "sg:person.01064621613.51", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01064621613.51"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/978-1-4615-6349-5_8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001151984", 
          "https://doi.org/10.1007/978-1-4615-6349-5_8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-4615-6349-5_8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001151984", 
          "https://doi.org/10.1007/978-1-4615-6349-5_8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.87.9.3584", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002564071"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0167-7799(96)10054-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020931848"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0167-7799(96)10047-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020997447"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0994-909", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022671474", 
          "https://doi.org/10.1038/nbt0994-909"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bp00020a600", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022680066"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.88.15.6760", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029680938"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1600-0609.1992.tb00053.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029844221"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1600-0609.1992.tb00053.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029844221"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.bmt.1701088", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033528691", 
          "https://doi.org/10.1038/sj.bmt.1701088"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.bmt.1701088", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033528691", 
          "https://doi.org/10.1038/sj.bmt.1701088"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/b978-0-7506-0421-5.50107-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040800196"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00744329", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042080431", 
          "https://doi.org/10.1007/bf00744329"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00744329", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042080431", 
          "https://doi.org/10.1007/bf00744329"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00749973", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044147241", 
          "https://doi.org/10.1007/bf00749973"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00749973", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044147241", 
          "https://doi.org/10.1007/bf00749973"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bp970136+", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047796684"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s001520050028", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1054512322", 
          "https://doi.org/10.1007/s001520050028"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/scd.1.1995.4.159", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059309058"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1076036821", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1076816089", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077022755", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077328439", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082464214", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082600390", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082759788", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082774347", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083081847", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1999-07", 
    "datePublishedReg": "1999-07-01", 
    "description": "The ex vivo expansion of hematopoietic progenitor cells is of great interest for a variety of clinical applications, e.g. bone marrow transplantation or gene therapy. Therefore it is of general interest to develop a culture system, able to mimic the in vivo hematopoesis, which is a prerequisite for long-term hematopoietic culture. Our approach was to modify a continuously perfused bioreactor for cultivation and expansion of human hematopoietic stem cells. Therefore we immobilized stromal cells (human primary stromal cells or the murine cell line M2-10B4) in porous glass carriers in a fixed bed reactor and cocultivated human hematopoietic progenitor cells for several weeks. After inoculation of mononuclear cells derived from umbilical cord blood or peripheral blood stem cells both adherent and non adherent cells were harvested and analyzed by flow cytometry and short-term colony assays. During cultivation there was a permanent production of progenitor cells and mature blood cells derived from the immobilized cells in the carriers. We could demonstrate the immobilization of hematopoietic progenitor cells of the myeloid system detectable in short-term colony assays. Additionally we could observe the expansion of very early progenitor cells (CFU-GEMM) up to 4.2-fold and later progenitor cells (CFU-GM and BFU-E) up to 7-fold and 1.8-fold, respectively.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1023/a:1008085932764", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1086307", 
        "issn": [
          "1381-5741", 
          "1573-0603"
        ], 
        "name": "Cytotechnology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1-3", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "30"
      }
    ], 
    "name": "Development of a fixed bed bioreactor for the expansion of human hematopoietic progenitor cells", 
    "pagination": "227-234", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "640bcfb1ba96edf6985d9ba5491232bb861151699f2d24bc657441bfcd1c5128"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "19003372"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "8807027"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1023/a:1008085932764"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1020997857"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1023/a:1008085932764", 
      "https://app.dimensions.ai/details/publication/pub.1020997857"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T22:29", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8690_00000499.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1023/A:1008085932764"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1023/a:1008085932764'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1023/a:1008085932764'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1023/a:1008085932764'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1023/a:1008085932764'


 

This table displays all metadata directly associated to this object as RDF triples.

159 TRIPLES      21 PREDICATES      53 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1023/a:1008085932764 schema:about anzsrc-for:11
2 anzsrc-for:1102
3 schema:author N2ec7ebf47e4f4f4ca3a668507d804641
4 schema:citation sg:pub.10.1007/978-1-4615-6349-5_8
5 sg:pub.10.1007/bf00744329
6 sg:pub.10.1007/bf00749973
7 sg:pub.10.1007/s001520050028
8 sg:pub.10.1038/nbt0994-909
9 sg:pub.10.1038/sj.bmt.1701088
10 https://app.dimensions.ai/details/publication/pub.1076036821
11 https://app.dimensions.ai/details/publication/pub.1076816089
12 https://app.dimensions.ai/details/publication/pub.1077022755
13 https://app.dimensions.ai/details/publication/pub.1077328439
14 https://app.dimensions.ai/details/publication/pub.1082464214
15 https://app.dimensions.ai/details/publication/pub.1082600390
16 https://app.dimensions.ai/details/publication/pub.1082759788
17 https://app.dimensions.ai/details/publication/pub.1082774347
18 https://app.dimensions.ai/details/publication/pub.1083081847
19 https://doi.org/10.1016/0167-7799(96)10047-0
20 https://doi.org/10.1016/0167-7799(96)10054-8
21 https://doi.org/10.1016/b978-0-7506-0421-5.50107-0
22 https://doi.org/10.1021/bp00020a600
23 https://doi.org/10.1021/bp970136+
24 https://doi.org/10.1073/pnas.87.9.3584
25 https://doi.org/10.1073/pnas.88.15.6760
26 https://doi.org/10.1089/scd.1.1995.4.159
27 https://doi.org/10.1111/j.1600-0609.1992.tb00053.x
28 schema:datePublished 1999-07
29 schema:datePublishedReg 1999-07-01
30 schema:description The ex vivo expansion of hematopoietic progenitor cells is of great interest for a variety of clinical applications, e.g. bone marrow transplantation or gene therapy. Therefore it is of general interest to develop a culture system, able to mimic the in vivo hematopoesis, which is a prerequisite for long-term hematopoietic culture. Our approach was to modify a continuously perfused bioreactor for cultivation and expansion of human hematopoietic stem cells. Therefore we immobilized stromal cells (human primary stromal cells or the murine cell line M2-10B4) in porous glass carriers in a fixed bed reactor and cocultivated human hematopoietic progenitor cells for several weeks. After inoculation of mononuclear cells derived from umbilical cord blood or peripheral blood stem cells both adherent and non adherent cells were harvested and analyzed by flow cytometry and short-term colony assays. During cultivation there was a permanent production of progenitor cells and mature blood cells derived from the immobilized cells in the carriers. We could demonstrate the immobilization of hematopoietic progenitor cells of the myeloid system detectable in short-term colony assays. Additionally we could observe the expansion of very early progenitor cells (CFU-GEMM) up to 4.2-fold and later progenitor cells (CFU-GM and BFU-E) up to 7-fold and 1.8-fold, respectively.
31 schema:genre research_article
32 schema:inLanguage en
33 schema:isAccessibleForFree false
34 schema:isPartOf N2b3808d299f1403c9e2cedf46b25b619
35 N62a98e831d57471c89418ebde4035ea3
36 sg:journal.1086307
37 schema:name Development of a fixed bed bioreactor for the expansion of human hematopoietic progenitor cells
38 schema:pagination 227-234
39 schema:productId N04a41b158ace4c0eaa322ac6733b77d4
40 N29168d8a52d4450dae4a6bd6ffe576f1
41 N388e3e0d3ad14469adb944b1a8de6f2d
42 N4416e910b9c4479993b455c08778652d
43 N61549637ea4045b6931ac8f874d3bf81
44 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020997857
45 https://doi.org/10.1023/a:1008085932764
46 schema:sdDatePublished 2019-04-10T22:29
47 schema:sdLicense https://scigraph.springernature.com/explorer/license/
48 schema:sdPublisher N5292f4ce0d404ca69209f4bb47388c29
49 schema:url http://link.springer.com/10.1023/A:1008085932764
50 sgo:license sg:explorer/license/
51 sgo:sdDataset articles
52 rdf:type schema:ScholarlyArticle
53 N04a41b158ace4c0eaa322ac6733b77d4 schema:name dimensions_id
54 schema:value pub.1020997857
55 rdf:type schema:PropertyValue
56 N11a514080ab24ddf97347a74ca3c6763 rdf:first sg:person.0647717652.06
57 rdf:rest Nce782e0ce5c841d2a0b3f21d986ef09c
58 N29168d8a52d4450dae4a6bd6ffe576f1 schema:name doi
59 schema:value 10.1023/a:1008085932764
60 rdf:type schema:PropertyValue
61 N2b3808d299f1403c9e2cedf46b25b619 schema:volumeNumber 30
62 rdf:type schema:PublicationVolume
63 N2ec7ebf47e4f4f4ca3a668507d804641 rdf:first sg:person.01325210552.08
64 rdf:rest N8d02ccda5f5d42d9885a609cc1a492f5
65 N388e3e0d3ad14469adb944b1a8de6f2d schema:name pubmed_id
66 schema:value 19003372
67 rdf:type schema:PropertyValue
68 N4416e910b9c4479993b455c08778652d schema:name readcube_id
69 schema:value 640bcfb1ba96edf6985d9ba5491232bb861151699f2d24bc657441bfcd1c5128
70 rdf:type schema:PropertyValue
71 N5292f4ce0d404ca69209f4bb47388c29 schema:name Springer Nature - SN SciGraph project
72 rdf:type schema:Organization
73 N61549637ea4045b6931ac8f874d3bf81 schema:name nlm_unique_id
74 schema:value 8807027
75 rdf:type schema:PropertyValue
76 N62a98e831d57471c89418ebde4035ea3 schema:issueNumber 1-3
77 rdf:type schema:PublicationIssue
78 N8d02ccda5f5d42d9885a609cc1a492f5 rdf:first sg:person.0601604452.95
79 rdf:rest N11a514080ab24ddf97347a74ca3c6763
80 Nce782e0ce5c841d2a0b3f21d986ef09c rdf:first sg:person.01064621613.51
81 rdf:rest rdf:nil
82 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
83 schema:name Medical and Health Sciences
84 rdf:type schema:DefinedTerm
85 anzsrc-for:1102 schema:inDefinedTermSet anzsrc-for:
86 schema:name Cardiorespiratory Medicine and Haematology
87 rdf:type schema:DefinedTerm
88 sg:journal.1086307 schema:issn 1381-5741
89 1573-0603
90 schema:name Cytotechnology
91 rdf:type schema:Periodical
92 sg:person.01064621613.51 schema:affiliation https://www.grid.ac/institutes/grid.8385.6
93 schema:familyName Biselli
94 schema:givenName Manfred
95 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01064621613.51
96 rdf:type schema:Person
97 sg:person.01325210552.08 schema:affiliation https://www.grid.ac/institutes/grid.8385.6
98 schema:familyName Meissner
99 schema:givenName Petra
100 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01325210552.08
101 rdf:type schema:Person
102 sg:person.0601604452.95 schema:affiliation https://www.grid.ac/institutes/grid.8385.6
103 schema:familyName Schröder
104 schema:givenName Bernd
105 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0601604452.95
106 rdf:type schema:Person
107 sg:person.0647717652.06 schema:affiliation https://www.grid.ac/institutes/grid.8385.6
108 schema:familyName Herfurth
109 schema:givenName Cornelia
110 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0647717652.06
111 rdf:type schema:Person
112 sg:pub.10.1007/978-1-4615-6349-5_8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001151984
113 https://doi.org/10.1007/978-1-4615-6349-5_8
114 rdf:type schema:CreativeWork
115 sg:pub.10.1007/bf00744329 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042080431
116 https://doi.org/10.1007/bf00744329
117 rdf:type schema:CreativeWork
118 sg:pub.10.1007/bf00749973 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044147241
119 https://doi.org/10.1007/bf00749973
120 rdf:type schema:CreativeWork
121 sg:pub.10.1007/s001520050028 schema:sameAs https://app.dimensions.ai/details/publication/pub.1054512322
122 https://doi.org/10.1007/s001520050028
123 rdf:type schema:CreativeWork
124 sg:pub.10.1038/nbt0994-909 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022671474
125 https://doi.org/10.1038/nbt0994-909
126 rdf:type schema:CreativeWork
127 sg:pub.10.1038/sj.bmt.1701088 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033528691
128 https://doi.org/10.1038/sj.bmt.1701088
129 rdf:type schema:CreativeWork
130 https://app.dimensions.ai/details/publication/pub.1076036821 schema:CreativeWork
131 https://app.dimensions.ai/details/publication/pub.1076816089 schema:CreativeWork
132 https://app.dimensions.ai/details/publication/pub.1077022755 schema:CreativeWork
133 https://app.dimensions.ai/details/publication/pub.1077328439 schema:CreativeWork
134 https://app.dimensions.ai/details/publication/pub.1082464214 schema:CreativeWork
135 https://app.dimensions.ai/details/publication/pub.1082600390 schema:CreativeWork
136 https://app.dimensions.ai/details/publication/pub.1082759788 schema:CreativeWork
137 https://app.dimensions.ai/details/publication/pub.1082774347 schema:CreativeWork
138 https://app.dimensions.ai/details/publication/pub.1083081847 schema:CreativeWork
139 https://doi.org/10.1016/0167-7799(96)10047-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020997447
140 rdf:type schema:CreativeWork
141 https://doi.org/10.1016/0167-7799(96)10054-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020931848
142 rdf:type schema:CreativeWork
143 https://doi.org/10.1016/b978-0-7506-0421-5.50107-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040800196
144 rdf:type schema:CreativeWork
145 https://doi.org/10.1021/bp00020a600 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022680066
146 rdf:type schema:CreativeWork
147 https://doi.org/10.1021/bp970136+ schema:sameAs https://app.dimensions.ai/details/publication/pub.1047796684
148 rdf:type schema:CreativeWork
149 https://doi.org/10.1073/pnas.87.9.3584 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002564071
150 rdf:type schema:CreativeWork
151 https://doi.org/10.1073/pnas.88.15.6760 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029680938
152 rdf:type schema:CreativeWork
153 https://doi.org/10.1089/scd.1.1995.4.159 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059309058
154 rdf:type schema:CreativeWork
155 https://doi.org/10.1111/j.1600-0609.1992.tb00053.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1029844221
156 rdf:type schema:CreativeWork
157 https://www.grid.ac/institutes/grid.8385.6 schema:alternateName Forschungszentrum Jülich
158 schema:name Institute of Biotechnology 2, Forschungszentrum Jülich, 52425, Jülich GmbH, Germany
159 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...