Enhancement of cytokine expression in transiently transfected cells by magnetoliposome mediated hyperthermia View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1997-11

AUTHORS

Isabelle Anne Bouhon, Masashige Shinkai, Hiroyuki Honda, Takeshi Kobayashi

ABSTRACT

We investigated the enhancement of cytokine expression by heat treatment in transiently transfected glioma cells. The cells were transfected with plasmid bearing the interferon (IFN)-β gene under control of the MMTV promoter, which is inducible by glucocorticoid (dexamethasone). Then magnetite particles (10 nm diameter) as intracellular heating material were incorporated to the cells as the form of magnetoliposome. After 5 hours of incorporation, alternative magnetic field (384Oe, 118kHz) was applied for intracellular heating. IFN-β secreted in the medium was assayed and its concentration was compared to the extracellular heating induced expression, both in the presence and absence of dexamethasone. Higher IFN-β concentration was detected in intracellular heating even at lower temperature, 39 °C, than 43 °C in extracellular heating. The IFN-β expression level reached in the presence of dexamethasone was about three times higher than in the absence of inducer. In intracellular heating of 60 min, the surviving cell number reduced until 20%. More... »

PAGES

231-234

Journal

TITLE

Cytotechnology

ISSUE

1-3

VOLUME

25

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1007930925862

DOI

http://dx.doi.org/10.1023/a:1007930925862

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1001795427

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22358897


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