Effect of Okadaic Acid, a Protein Phosphatase Inhibitor, on Heat Stress-Induced HSP72 Synthesis and Thermotolerance View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-06

AUTHORS

Marie Joyeux, Claire Arnaud, Marie-Jeanne Richard, Derek M. Yellon, Pierre Demenge, Christophe Ribuot

ABSTRACT

Heat stress proteins (HSPs), in particular HSP72, seem to play a major role in cell protection against lethal stresses such as hyperthermia or ischemia. HSP synthesis is negatively regulated by protein phosphatases, which are implicated in dephosphorylation processes. In the present study, we have investigated the effect of okadaic acid (OA, a protein phosphatase inhibitor) on heat stress-induced HSP72 synthesis and thermotolerance in smooth muscle cells (SMC). SMC were heat stressed (42 degrees C for 20 minutes) in the presence of 250 nM OA (HS+OA cells) or its vehicle (HS+V cells). Control (OA or V) cells were not heat stressed. HSP72 mRNA expression was determined 1, 1.5, 3, and 6 hours after heat stress by RT-PCR, and HSP72 synthesis was determined 6, 12, 24, 48, and 72 hours after heat stress by Western blotting. SMC survival of lethal hyperthermia (47 degrees C for 90 minutes) was assessed 6, 24, and 48 hours after heat stress by a tetrazolium assay. The maximal expression of HSP72 mRNA was markedly prolonged in HS+OA cells (until 6 hours after heat stress) compared to HS+V cells (1 hour after heat stress). The kinetics of HSP72 synthesis and thermotolerance of SMC were not different between HS+OA and HS+V cells. Baseline HSP72 mRNA and protein expression were similar in control V and OA cells. In conclusion, okadaic acid treatment of SMC potentiated HSP72 mRNA expression without affecting heat stress-induced HSP72 synthesis and thermotolerance. More... »

PAGES

441-446

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1007824502285

DOI

http://dx.doi.org/10.1023/a:1007824502285

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1016674314

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/10999652


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Enzyme Inhibitors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fever", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Heat-Shock Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hot Temperature", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Muscle, Smooth, Vascular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Okadaic Acid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phosphoric Monoester Hydrolases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rats", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rats, Wistar", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Reverse Transcriptase Polymerase Chain Reaction", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "name": [
            "Laboratoire de Pharmacologie Cardiovasculaire Exp\u00e9rimentale-Biomol\u00e9cules, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Joyeux", 
        "givenName": "Marie", 
        "id": "sg:person.01273200765.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01273200765.00"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Laboratoire de Pharmacologie Cardiovasculaire Exp\u00e9rimentale-Biomol\u00e9cules, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Arnaud", 
        "givenName": "Claire", 
        "id": "sg:person.01140105146.11", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01140105146.11"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Joseph Fourier University", 
          "id": "https://www.grid.ac/institutes/grid.9621.c", 
          "name": [
            "Laboratoire de Biologie du stress oxydant, Universit\u00e9 Joseph Fourier, U.F.R. de Pharmacie, Grenoble, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Richard", 
        "givenName": "Marie-Jeanne", 
        "id": "sg:person.013242257222.96", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013242257222.96"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "The Hatter Institute for Cardiovascular Studies, University College London Hospitals & Medical School, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yellon", 
        "givenName": "Derek M.", 
        "id": "sg:person.011334646637.52", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011334646637.52"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Laboratoire de Pharmacologie Cardiovasculaire Exp\u00e9rimentale-Biomol\u00e9cules, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Demenge", 
        "givenName": "Pierre", 
        "id": "sg:person.0113712666.51", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0113712666.51"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Laboratoire de Pharmacologie Cardiovasculaire Exp\u00e9rimentale-Biomol\u00e9cules, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ribuot", 
        "givenName": "Christophe", 
        "id": "sg:person.01312672721.34", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01312672721.34"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/335372a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000571263", 
          "https://doi.org/10.1038/335372a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-1033.1991.tb16088.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003097486"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1139/o91-057", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005226181"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.88.5.1681", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007202653"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci117815", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007668032"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/227680a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010419937", 
          "https://doi.org/10.1038/227680a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci116594", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020225693"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.92.6.2126", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021334061"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jmcc.1994.1084", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021993203"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02789101", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023547592", 
          "https://doi.org/10.1007/bf02789101"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02789101", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023547592", 
          "https://doi.org/10.1007/bf02789101"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm1097-1150", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024254744", 
          "https://doi.org/10.1038/nm1097-1150"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/sj.bjp.0702146", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024964051"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci119124", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027600765"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcb.240540207", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030140785"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcb.240540207", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030140785"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1097-4644(19960501)61:2<255::aid-jcb9>3.0.co;2-t", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030427835"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0076-6879(91)01042-z", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034194144"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci117865", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037332095"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jmcc.1996.0227", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037780375"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0022-2828(08)80006-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037916443"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.93.6.2339", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038249175"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0163-7258(98)00028-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038992984"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci117030", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039336195"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci118468", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041760004"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1546322", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062490329"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.2188360", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062525949"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.8421783", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062655517"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1161/01.cir.94.6.1408", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1063337495"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3892/ijmm.2.4.375", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1071506676"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079959686", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082783105", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/oxfordjournals.jbchem.a124956", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082910731"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/ajpcell.1996.270.4.c1017", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083002942"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1096/fasebj.12.6.451", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083240444"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2000-06", 
    "datePublishedReg": "2000-06-01", 
    "description": "Heat stress proteins (HSPs), in particular HSP72, seem to play a major role in cell protection against lethal stresses such as hyperthermia or ischemia. HSP synthesis is negatively regulated by protein phosphatases, which are implicated in dephosphorylation processes. In the present study, we have investigated the effect of okadaic acid (OA, a protein phosphatase inhibitor) on heat stress-induced HSP72 synthesis and thermotolerance in smooth muscle cells (SMC). SMC were heat stressed (42 degrees C for 20 minutes) in the presence of 250 nM OA (HS+OA cells) or its vehicle (HS+V cells). Control (OA or V) cells were not heat stressed. HSP72 mRNA expression was determined 1, 1.5, 3, and 6 hours after heat stress by RT-PCR, and HSP72 synthesis was determined 6, 12, 24, 48, and 72 hours after heat stress by Western blotting. SMC survival of lethal hyperthermia (47 degrees C for 90 minutes) was assessed 6, 24, and 48 hours after heat stress by a tetrazolium assay. The maximal expression of HSP72 mRNA was markedly prolonged in HS+OA cells (until 6 hours after heat stress) compared to HS+V cells (1 hour after heat stress). The kinetics of HSP72 synthesis and thermotolerance of SMC were not different between HS+OA and HS+V cells. Baseline HSP72 mRNA and protein expression were similar in control V and OA cells. In conclusion, okadaic acid treatment of SMC potentiated HSP72 mRNA expression without affecting heat stress-induced HSP72 synthesis and thermotolerance.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1023/a:1007824502285", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1097576", 
        "issn": [
          "0920-3206", 
          "1573-7241"
        ], 
        "name": "Cardiovascular Drugs and Therapy", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "14"
      }
    ], 
    "name": "Effect of Okadaic Acid, a Protein Phosphatase Inhibitor, on Heat Stress-Induced HSP72 Synthesis and Thermotolerance", 
    "pagination": "441-446", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "4f72914b18c89e2211c178cdf856574956678e923f8436da435afe80dd6a715d"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "10999652"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "8712220"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1023/a:1007824502285"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1016674314"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1023/a:1007824502285", 
      "https://app.dimensions.ai/details/publication/pub.1016674314"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T00:10", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8695_00000487.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1023/A:1007824502285"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1023/a:1007824502285'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1023/a:1007824502285'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1023/a:1007824502285'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1023/a:1007824502285'


 

This table displays all metadata directly associated to this object as RDF triples.

263 TRIPLES      21 PREDICATES      74 URIs      33 LITERALS      21 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1023/a:1007824502285 schema:about N039ce058ea8540a6837cf74941cd8a21
2 N2bfb5be0c5da471ca4b5dd697d4049e8
3 N2dca576a2643487a85e15427650d51e9
4 N41b588081afc498ca6d1e9567e4f1705
5 N4ec0a986012544a3a818988bd8d1d426
6 N6b71b9413ff2444396d19060aea90bbb
7 N9b27eaeb84fd4adb91ca0d118472a6d1
8 N9d4abf4d6de541a3b956452704cef1c9
9 Na5d2a7c90a9a44a5910710926549a107
10 Nb23de75a230c4c068d3fdc351d14c7b5
11 Nd29d61507791497eb5e7682deb8a8ffd
12 Nde99fa1a0a6a43429a0b3b36d9681717
13 anzsrc-for:06
14 anzsrc-for:0601
15 schema:author N04379fea8d564ddaaca53e9beafabf47
16 schema:citation sg:pub.10.1007/bf02789101
17 sg:pub.10.1038/227680a0
18 sg:pub.10.1038/335372a0
19 sg:pub.10.1038/nm1097-1150
20 https://app.dimensions.ai/details/publication/pub.1079959686
21 https://app.dimensions.ai/details/publication/pub.1082783105
22 https://doi.org/10.1002/(sici)1097-4644(19960501)61:2<255::aid-jcb9>3.0.co;2-t
23 https://doi.org/10.1002/jcb.240540207
24 https://doi.org/10.1006/jmcc.1994.1084
25 https://doi.org/10.1006/jmcc.1996.0227
26 https://doi.org/10.1016/0076-6879(91)01042-z
27 https://doi.org/10.1016/s0022-2828(08)80006-5
28 https://doi.org/10.1016/s0163-7258(98)00028-x
29 https://doi.org/10.1038/sj.bjp.0702146
30 https://doi.org/10.1073/pnas.88.5.1681
31 https://doi.org/10.1073/pnas.92.6.2126
32 https://doi.org/10.1073/pnas.93.6.2339
33 https://doi.org/10.1093/oxfordjournals.jbchem.a124956
34 https://doi.org/10.1096/fasebj.12.6.451
35 https://doi.org/10.1111/j.1432-1033.1991.tb16088.x
36 https://doi.org/10.1126/science.1546322
37 https://doi.org/10.1126/science.2188360
38 https://doi.org/10.1126/science.8421783
39 https://doi.org/10.1139/o91-057
40 https://doi.org/10.1152/ajpcell.1996.270.4.c1017
41 https://doi.org/10.1161/01.cir.94.6.1408
42 https://doi.org/10.1172/jci116594
43 https://doi.org/10.1172/jci117030
44 https://doi.org/10.1172/jci117815
45 https://doi.org/10.1172/jci117865
46 https://doi.org/10.1172/jci118468
47 https://doi.org/10.1172/jci119124
48 https://doi.org/10.3892/ijmm.2.4.375
49 schema:datePublished 2000-06
50 schema:datePublishedReg 2000-06-01
51 schema:description Heat stress proteins (HSPs), in particular HSP72, seem to play a major role in cell protection against lethal stresses such as hyperthermia or ischemia. HSP synthesis is negatively regulated by protein phosphatases, which are implicated in dephosphorylation processes. In the present study, we have investigated the effect of okadaic acid (OA, a protein phosphatase inhibitor) on heat stress-induced HSP72 synthesis and thermotolerance in smooth muscle cells (SMC). SMC were heat stressed (42 degrees C for 20 minutes) in the presence of 250 nM OA (HS+OA cells) or its vehicle (HS+V cells). Control (OA or V) cells were not heat stressed. HSP72 mRNA expression was determined 1, 1.5, 3, and 6 hours after heat stress by RT-PCR, and HSP72 synthesis was determined 6, 12, 24, 48, and 72 hours after heat stress by Western blotting. SMC survival of lethal hyperthermia (47 degrees C for 90 minutes) was assessed 6, 24, and 48 hours after heat stress by a tetrazolium assay. The maximal expression of HSP72 mRNA was markedly prolonged in HS+OA cells (until 6 hours after heat stress) compared to HS+V cells (1 hour after heat stress). The kinetics of HSP72 synthesis and thermotolerance of SMC were not different between HS+OA and HS+V cells. Baseline HSP72 mRNA and protein expression were similar in control V and OA cells. In conclusion, okadaic acid treatment of SMC potentiated HSP72 mRNA expression without affecting heat stress-induced HSP72 synthesis and thermotolerance.
52 schema:genre research_article
53 schema:inLanguage en
54 schema:isAccessibleForFree false
55 schema:isPartOf N1800fdcf34574140997cfc7c81832079
56 Nf4ad4462285d4694a335860084e2fd05
57 sg:journal.1097576
58 schema:name Effect of Okadaic Acid, a Protein Phosphatase Inhibitor, on Heat Stress-Induced HSP72 Synthesis and Thermotolerance
59 schema:pagination 441-446
60 schema:productId N128a64718677418db4e2d1931a53572c
61 N5dfb05ea98004ee681da88545582920f
62 N6087047b66844c3dbdeb611bdcc6ab43
63 Nbdbd8fdd0fda405d8d0fee069051a99f
64 Ncc541e6e2711426ca299e3e23bdc2e75
65 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016674314
66 https://doi.org/10.1023/a:1007824502285
67 schema:sdDatePublished 2019-04-11T00:10
68 schema:sdLicense https://scigraph.springernature.com/explorer/license/
69 schema:sdPublisher Naf29070316de47ce9900063867e12cdc
70 schema:url http://link.springer.com/10.1023/A:1007824502285
71 sgo:license sg:explorer/license/
72 sgo:sdDataset articles
73 rdf:type schema:ScholarlyArticle
74 N039ce058ea8540a6837cf74941cd8a21 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
75 schema:name Reverse Transcriptase Polymerase Chain Reaction
76 rdf:type schema:DefinedTerm
77 N04379fea8d564ddaaca53e9beafabf47 rdf:first sg:person.01273200765.00
78 rdf:rest N35bcbc418ac44e2a8ab9a8298cf943f9
79 N0a85251e2b5044cdb8210d2003a04bb2 schema:name Laboratoire de Pharmacologie Cardiovasculaire Expérimentale-Biomolécules, France
80 rdf:type schema:Organization
81 N128a64718677418db4e2d1931a53572c schema:name nlm_unique_id
82 schema:value 8712220
83 rdf:type schema:PropertyValue
84 N1800fdcf34574140997cfc7c81832079 schema:issueNumber 4
85 rdf:type schema:PublicationIssue
86 N2bfb5be0c5da471ca4b5dd697d4049e8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
87 schema:name Animals
88 rdf:type schema:DefinedTerm
89 N2dca576a2643487a85e15427650d51e9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Hot Temperature
91 rdf:type schema:DefinedTerm
92 N2ef610356b2f414e885c060e49dbf877 schema:name Laboratoire de Pharmacologie Cardiovasculaire Expérimentale-Biomolécules, France
93 rdf:type schema:Organization
94 N35bcbc418ac44e2a8ab9a8298cf943f9 rdf:first sg:person.01140105146.11
95 rdf:rest N4897c4f53d534db0972386d1de41f7a7
96 N41b588081afc498ca6d1e9567e4f1705 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Enzyme Inhibitors
98 rdf:type schema:DefinedTerm
99 N4897c4f53d534db0972386d1de41f7a7 rdf:first sg:person.013242257222.96
100 rdf:rest N74a91dd0a7af459da146852d3feb34fc
101 N4ec0a986012544a3a818988bd8d1d426 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
102 schema:name Rats
103 rdf:type schema:DefinedTerm
104 N5dfb05ea98004ee681da88545582920f schema:name readcube_id
105 schema:value 4f72914b18c89e2211c178cdf856574956678e923f8436da435afe80dd6a715d
106 rdf:type schema:PropertyValue
107 N6087047b66844c3dbdeb611bdcc6ab43 schema:name dimensions_id
108 schema:value pub.1016674314
109 rdf:type schema:PropertyValue
110 N6a8e6c81fb3b47c2b02e8c8eac609efe schema:name Laboratoire de Pharmacologie Cardiovasculaire Expérimentale-Biomolécules, France
111 rdf:type schema:Organization
112 N6b71b9413ff2444396d19060aea90bbb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Phosphoric Monoester Hydrolases
114 rdf:type schema:DefinedTerm
115 N74a91dd0a7af459da146852d3feb34fc rdf:first sg:person.011334646637.52
116 rdf:rest N9d72925ed2ef495591d98f3de607393a
117 N8837df4fbb504fcf9def005f37799add schema:name Laboratoire de Pharmacologie Cardiovasculaire Expérimentale-Biomolécules, France
118 rdf:type schema:Organization
119 N9b27eaeb84fd4adb91ca0d118472a6d1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Male
121 rdf:type schema:DefinedTerm
122 N9d4abf4d6de541a3b956452704cef1c9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name Okadaic Acid
124 rdf:type schema:DefinedTerm
125 N9d72925ed2ef495591d98f3de607393a rdf:first sg:person.0113712666.51
126 rdf:rest Na5c1407ff0fd4c88bb060f89b1a883c2
127 Na5c1407ff0fd4c88bb060f89b1a883c2 rdf:first sg:person.01312672721.34
128 rdf:rest rdf:nil
129 Na5d2a7c90a9a44a5910710926549a107 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Rats, Wistar
131 rdf:type schema:DefinedTerm
132 Naf29070316de47ce9900063867e12cdc schema:name Springer Nature - SN SciGraph project
133 rdf:type schema:Organization
134 Nb23de75a230c4c068d3fdc351d14c7b5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Muscle, Smooth, Vascular
136 rdf:type schema:DefinedTerm
137 Nbdbd8fdd0fda405d8d0fee069051a99f schema:name pubmed_id
138 schema:value 10999652
139 rdf:type schema:PropertyValue
140 Ncc541e6e2711426ca299e3e23bdc2e75 schema:name doi
141 schema:value 10.1023/a:1007824502285
142 rdf:type schema:PropertyValue
143 Nd29d61507791497eb5e7682deb8a8ffd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Fever
145 rdf:type schema:DefinedTerm
146 Nde99fa1a0a6a43429a0b3b36d9681717 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Heat-Shock Proteins
148 rdf:type schema:DefinedTerm
149 Ne6543a59aadf4b3293ae50f777c745b9 schema:name The Hatter Institute for Cardiovascular Studies, University College London Hospitals & Medical School, London, UK
150 rdf:type schema:Organization
151 Nf4ad4462285d4694a335860084e2fd05 schema:volumeNumber 14
152 rdf:type schema:PublicationVolume
153 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
154 schema:name Biological Sciences
155 rdf:type schema:DefinedTerm
156 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
157 schema:name Biochemistry and Cell Biology
158 rdf:type schema:DefinedTerm
159 sg:journal.1097576 schema:issn 0920-3206
160 1573-7241
161 schema:name Cardiovascular Drugs and Therapy
162 rdf:type schema:Periodical
163 sg:person.011334646637.52 schema:affiliation Ne6543a59aadf4b3293ae50f777c745b9
164 schema:familyName Yellon
165 schema:givenName Derek M.
166 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011334646637.52
167 rdf:type schema:Person
168 sg:person.0113712666.51 schema:affiliation N2ef610356b2f414e885c060e49dbf877
169 schema:familyName Demenge
170 schema:givenName Pierre
171 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0113712666.51
172 rdf:type schema:Person
173 sg:person.01140105146.11 schema:affiliation N6a8e6c81fb3b47c2b02e8c8eac609efe
174 schema:familyName Arnaud
175 schema:givenName Claire
176 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01140105146.11
177 rdf:type schema:Person
178 sg:person.01273200765.00 schema:affiliation N8837df4fbb504fcf9def005f37799add
179 schema:familyName Joyeux
180 schema:givenName Marie
181 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01273200765.00
182 rdf:type schema:Person
183 sg:person.01312672721.34 schema:affiliation N0a85251e2b5044cdb8210d2003a04bb2
184 schema:familyName Ribuot
185 schema:givenName Christophe
186 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01312672721.34
187 rdf:type schema:Person
188 sg:person.013242257222.96 schema:affiliation https://www.grid.ac/institutes/grid.9621.c
189 schema:familyName Richard
190 schema:givenName Marie-Jeanne
191 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013242257222.96
192 rdf:type schema:Person
193 sg:pub.10.1007/bf02789101 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023547592
194 https://doi.org/10.1007/bf02789101
195 rdf:type schema:CreativeWork
196 sg:pub.10.1038/227680a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010419937
197 https://doi.org/10.1038/227680a0
198 rdf:type schema:CreativeWork
199 sg:pub.10.1038/335372a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000571263
200 https://doi.org/10.1038/335372a0
201 rdf:type schema:CreativeWork
202 sg:pub.10.1038/nm1097-1150 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024254744
203 https://doi.org/10.1038/nm1097-1150
204 rdf:type schema:CreativeWork
205 https://app.dimensions.ai/details/publication/pub.1079959686 schema:CreativeWork
206 https://app.dimensions.ai/details/publication/pub.1082783105 schema:CreativeWork
207 https://doi.org/10.1002/(sici)1097-4644(19960501)61:2<255::aid-jcb9>3.0.co;2-t schema:sameAs https://app.dimensions.ai/details/publication/pub.1030427835
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1002/jcb.240540207 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030140785
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1006/jmcc.1994.1084 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021993203
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1006/jmcc.1996.0227 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037780375
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1016/0076-6879(91)01042-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1034194144
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1016/s0022-2828(08)80006-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037916443
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1016/s0163-7258(98)00028-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1038992984
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1038/sj.bjp.0702146 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024964051
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1073/pnas.88.5.1681 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007202653
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1073/pnas.92.6.2126 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021334061
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1073/pnas.93.6.2339 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038249175
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1093/oxfordjournals.jbchem.a124956 schema:sameAs https://app.dimensions.ai/details/publication/pub.1082910731
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1096/fasebj.12.6.451 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083240444
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1111/j.1432-1033.1991.tb16088.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1003097486
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1126/science.1546322 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062490329
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1126/science.2188360 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062525949
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1126/science.8421783 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062655517
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1139/o91-057 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005226181
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1152/ajpcell.1996.270.4.c1017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083002942
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1161/01.cir.94.6.1408 schema:sameAs https://app.dimensions.ai/details/publication/pub.1063337495
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1172/jci116594 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020225693
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1172/jci117030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039336195
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1172/jci117815 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007668032
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1172/jci117865 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037332095
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1172/jci118468 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041760004
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1172/jci119124 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027600765
258 rdf:type schema:CreativeWork
259 https://doi.org/10.3892/ijmm.2.4.375 schema:sameAs https://app.dimensions.ai/details/publication/pub.1071506676
260 rdf:type schema:CreativeWork
261 https://www.grid.ac/institutes/grid.9621.c schema:alternateName Joseph Fourier University
262 schema:name Laboratoire de Biologie du stress oxydant, Université Joseph Fourier, U.F.R. de Pharmacie, Grenoble, France
263 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...