In vitro α1-3 or α1-4 fucosylation of type I and II oligosaccharides with secreted forms of recombinant human fucosyltransferases III ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-09

AUTHORS

Manfred Nimtz, Eckart Grabenhorst, Ulrike Gambert, Julia Costa, Victor Wray, Michael Morr, Joachim Thiem, Harald S. Conradt

ABSTRACT

Transgalactosylation of chitobiose and chitotriose employing beta-galactosidase from bovine testes yielded mixtures with beta1-3 linked galactose (type I) and beta1-4 linked galactose (type II) in a final ratio of 1:1 for the tri- and 1:1.4 for the tetrasaccharide. After 24 h incubations of the two purified oligosaccharide mixtures with large amounts (20-fold increase compared with standard conditions) of human alpha1,3/4-fucosyltransferase III (FucT III), the type I tri-/tetrasaccharides were completely converted to the Lewis(a) structure, whereas approximately 10% fucosylation of the type II isomers to the Lewis(x) oligosaccharides was observed in long-term incubations. Employing large amounts of human alpha1,3-fucosyltransferase VI (FucT VI), the type I trisaccharide substrate was exclusively fucosylated at the proximal O-4 substituted N-acetylglucosamine (GlcNAc) (20%) whereas almost all of the type II isomers was converted to the corresponding Lewis(x) product. 45% of the type I tetrasaccharide was fucosylated at the second GlcNAc solely by FucT VI. The type II isomer was almost completely alpha1-3 fucosylated to yield the Lewisx derivative with traces of a structure that contained an additional fucose at the reducing GlcNAc. The results obtained in the present study employing high amounts of enzyme confirmed our previous results that FucT III acts preponderantly as a beta1-4 fucosyltransferase onto GlcNAc in vitro. Human FucT VI attaches fucose exclusively in an alpha1-3 linkage to 4-substituted GlcNAc in vitro and does not modify any 3-substituted GlcNAc to yield Lewis(a) oligosaccharides. With 8-methoxycarbonyloctyl glycoside acceptors used under standard conditions, FucT III acts exclusively on the type I and FucT VI only on the type II derivative. With lacto-N-tetraose, lacto-N-fucopentraose I, or LS-tetrasaccharide as substrates, FucT III modified the 3-substituted GlcNAc and the reducing glucose; FucT VI recognized only lacto-N-neotetraose as a substrate. More... »

PAGES

873-883

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1006907031940

DOI

http://dx.doi.org/10.1023/a:1006907031940

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1014590770

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/10052591


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1115", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Pharmacology and Pharmaceutical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Carbohydrate Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Disaccharides", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fucosyltransferases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Galactose", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lewis Blood-Group System", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Methylation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oligosaccharides", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombinant Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Substrate Specificity", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transfection", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Trisaccharides", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "GBF German Research Centre for Biotechnology", 
          "id": "https://www.grid.ac/institutes/grid.418123.d", 
          "name": [
            "Gesellschaft f\u00fcr Biotechnologische Forschung, Mascheroder Weg 1, 38124, Braunschweig, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nimtz", 
        "givenName": "Manfred", 
        "id": "sg:person.01310136664.45", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01310136664.45"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "GBF German Research Centre for Biotechnology", 
          "id": "https://www.grid.ac/institutes/grid.418123.d", 
          "name": [
            "Gesellschaft f\u00fcr Biotechnologische Forschung, Mascheroder Weg 1, 38124, Braunschweig, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Grabenhorst", 
        "givenName": "Eckart", 
        "id": "sg:person.01313334742.81", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01313334742.81"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Institut f\u00fcr Organische Chemie, Universit\u00e4t Hamburg, Martin-Luther-King Platz 6, 20146, Hamburg, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gambert", 
        "givenName": "Ulrike", 
        "id": "sg:person.01025224327.15", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01025224327.15"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "ITQB/IBET, Apartado 12, P-2780, Oeiras, Portugal"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Costa", 
        "givenName": "Julia", 
        "id": "sg:person.0622547010.34", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0622547010.34"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "GBF German Research Centre for Biotechnology", 
          "id": "https://www.grid.ac/institutes/grid.418123.d", 
          "name": [
            "Gesellschaft f\u00fcr Biotechnologische Forschung, Mascheroder Weg 1, 38124, Braunschweig, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wray", 
        "givenName": "Victor", 
        "id": "sg:person.01216175536.16", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01216175536.16"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "GBF German Research Centre for Biotechnology", 
          "id": "https://www.grid.ac/institutes/grid.418123.d", 
          "name": [
            "Gesellschaft f\u00fcr Biotechnologische Forschung, Mascheroder Weg 1, 38124, Braunschweig, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Morr", 
        "givenName": "Michael", 
        "id": "sg:person.014054115613.54", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014054115613.54"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Institut f\u00fcr Organische Chemie, Universit\u00e4t Hamburg, Martin-Luther-King Platz 6, 20146, Hamburg, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Thiem", 
        "givenName": "Joachim", 
        "id": "sg:person.011402553405.31", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011402553405.31"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "GBF German Research Centre for Biotechnology", 
          "id": "https://www.grid.ac/institutes/grid.418123.d", 
          "name": [
            "Gesellschaft f\u00fcr Biotechnologische Forschung, Mascheroder Weg 1, 38124, Braunschweig, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Conradt", 
        "givenName": "Harald S.", 
        "id": "sg:person.01346153110.13", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01346153110.13"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf00731137", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000547172", 
          "https://doi.org/10.1007/bf00731137"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00731137", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000547172", 
          "https://doi.org/10.1007/bf00731137"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.4.8.1288", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002160202"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.270.15.8712", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004916630"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-1033.1993.tb17732.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005024080"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-1033.1995.718zz.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005259043"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-5793(90)80361-l", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006175409"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00731136", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007244866", 
          "https://doi.org/10.1007/bf00731136"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00731136", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007244866", 
          "https://doi.org/10.1007/bf00731136"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0008-6215(96)00324-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013536924"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/glycob/7.7.921", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020337535"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0006-291x(92)90697-j", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021977142"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-1033.1993.tb18042.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026688297"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.272.17.11613", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032304279"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.91.16.7390", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034813465"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0014-5793(97)01121-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035053328"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(90)90444-j", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037836709"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.117.4.895", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052019930"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0006-291x(91)91875-d", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052172532"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0006-291x(05)81472-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053619695"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00199a016", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055164907"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00448a031", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055176669"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075699150", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/oxfordjournals.jbchem.a127869", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1076282982"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1998-09", 
    "datePublishedReg": "1998-09-01", 
    "description": "Transgalactosylation of chitobiose and chitotriose employing beta-galactosidase from bovine testes yielded mixtures with beta1-3 linked galactose (type I) and beta1-4 linked galactose (type II) in a final ratio of 1:1 for the tri- and 1:1.4 for the tetrasaccharide. After 24 h incubations of the two purified oligosaccharide mixtures with large amounts (20-fold increase compared with standard conditions) of human alpha1,3/4-fucosyltransferase III (FucT III), the type I tri-/tetrasaccharides were completely converted to the Lewis(a) structure, whereas approximately 10% fucosylation of the type II isomers to the Lewis(x) oligosaccharides was observed in long-term incubations. Employing large amounts of human alpha1,3-fucosyltransferase VI (FucT VI), the type I trisaccharide substrate was exclusively fucosylated at the proximal O-4 substituted N-acetylglucosamine (GlcNAc) (20%) whereas almost all of the type II isomers was converted to the corresponding Lewis(x) product. 45% of the type I tetrasaccharide was fucosylated at the second GlcNAc solely by FucT VI. The type II isomer was almost completely alpha1-3 fucosylated to yield the Lewisx derivative with traces of a structure that contained an additional fucose at the reducing GlcNAc. The results obtained in the present study employing high amounts of enzyme confirmed our previous results that FucT III acts preponderantly as a beta1-4 fucosyltransferase onto GlcNAc in vitro. Human FucT VI attaches fucose exclusively in an alpha1-3 linkage to 4-substituted GlcNAc in vitro and does not modify any 3-substituted GlcNAc to yield Lewis(a) oligosaccharides. With 8-methoxycarbonyloctyl glycoside acceptors used under standard conditions, FucT III acts exclusively on the type I and FucT VI only on the type II derivative. With lacto-N-tetraose, lacto-N-fucopentraose I, or LS-tetrasaccharide as substrates, FucT III modified the 3-substituted GlcNAc and the reducing glucose; FucT VI recognized only lacto-N-neotetraose as a substrate.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1023/a:1006907031940", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1096094", 
        "issn": [
          "0969-3653", 
          "1573-4986"
        ], 
        "name": "Glycoconjugate Journal", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "9", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "15"
      }
    ], 
    "name": "In vitro \u03b11-3 or \u03b11-4 fucosylation of type I and II oligosaccharides with secreted forms of recombinant human fucosyltransferases III and VI", 
    "pagination": "873-883", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "4582ababc0aebf255e2226e18a2fa5ae077816bfe0e8860283b3dba32b8240f5"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "10052591"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "8603310"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1023/a:1006907031940"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1014590770"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1023/a:1006907031940", 
      "https://app.dimensions.ai/details/publication/pub.1014590770"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T18:17", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8675_00000499.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1023/A:1006907031940"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1023/a:1006907031940'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1023/a:1006907031940'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1023/a:1006907031940'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1023/a:1006907031940'


 

This table displays all metadata directly associated to this object as RDF triples.

247 TRIPLES      21 PREDICATES      65 URIs      35 LITERALS      23 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1023/a:1006907031940 schema:about N1014872089ab402c8584afc168d83ce5
2 N1cb4e57739c6460e92a97212b758a28c
3 N2c690e35a79947da82a6b91f2172e40a
4 N36a165f7bd2a4756a8ae9c6c46f1c93b
5 N383f3ef9c92245ceba740c09b5e2f5f5
6 N4974f74cba4b45d098142aec48eeb6cd
7 N52f27d969e6b41c79e7d90ac9d67460d
8 N5519d409d7e0445f9171d72241228e4a
9 N55e9dcb13d2b4ff99fde7dbd95bc3732
10 N6e5c7ab6c53d4714a96c6a760e41bf9f
11 N8ef19e8ab2094fe19e601d28a5b7df83
12 Nc965b580b7b54b038d508adf12c738be
13 Nda7522b36e84400d9213a90d1fb11406
14 Nf55139499ab64ab9b161b088d3294ad6
15 anzsrc-for:11
16 anzsrc-for:1115
17 schema:author N9bcdc4049be945398f76571d6235abab
18 schema:citation sg:pub.10.1007/bf00731136
19 sg:pub.10.1007/bf00731137
20 https://app.dimensions.ai/details/publication/pub.1075699150
21 https://doi.org/10.1016/0006-291x(91)91875-d
22 https://doi.org/10.1016/0006-291x(92)90697-j
23 https://doi.org/10.1016/0014-5793(90)80361-l
24 https://doi.org/10.1016/0092-8674(90)90444-j
25 https://doi.org/10.1016/s0006-291x(05)81472-x
26 https://doi.org/10.1016/s0008-6215(96)00324-2
27 https://doi.org/10.1016/s0014-5793(97)01121-6
28 https://doi.org/10.1021/bi00199a016
29 https://doi.org/10.1021/bi00448a031
30 https://doi.org/10.1073/pnas.91.16.7390
31 https://doi.org/10.1074/jbc.270.15.8712
32 https://doi.org/10.1074/jbc.272.17.11613
33 https://doi.org/10.1083/jcb.117.4.895
34 https://doi.org/10.1093/glycob/7.7.921
35 https://doi.org/10.1093/oxfordjournals.jbchem.a127869
36 https://doi.org/10.1101/gad.4.8.1288
37 https://doi.org/10.1111/j.1432-1033.1993.tb17732.x
38 https://doi.org/10.1111/j.1432-1033.1993.tb18042.x
39 https://doi.org/10.1111/j.1432-1033.1995.718zz.x
40 schema:datePublished 1998-09
41 schema:datePublishedReg 1998-09-01
42 schema:description Transgalactosylation of chitobiose and chitotriose employing beta-galactosidase from bovine testes yielded mixtures with beta1-3 linked galactose (type I) and beta1-4 linked galactose (type II) in a final ratio of 1:1 for the tri- and 1:1.4 for the tetrasaccharide. After 24 h incubations of the two purified oligosaccharide mixtures with large amounts (20-fold increase compared with standard conditions) of human alpha1,3/4-fucosyltransferase III (FucT III), the type I tri-/tetrasaccharides were completely converted to the Lewis(a) structure, whereas approximately 10% fucosylation of the type II isomers to the Lewis(x) oligosaccharides was observed in long-term incubations. Employing large amounts of human alpha1,3-fucosyltransferase VI (FucT VI), the type I trisaccharide substrate was exclusively fucosylated at the proximal O-4 substituted N-acetylglucosamine (GlcNAc) (20%) whereas almost all of the type II isomers was converted to the corresponding Lewis(x) product. 45% of the type I tetrasaccharide was fucosylated at the second GlcNAc solely by FucT VI. The type II isomer was almost completely alpha1-3 fucosylated to yield the Lewisx derivative with traces of a structure that contained an additional fucose at the reducing GlcNAc. The results obtained in the present study employing high amounts of enzyme confirmed our previous results that FucT III acts preponderantly as a beta1-4 fucosyltransferase onto GlcNAc in vitro. Human FucT VI attaches fucose exclusively in an alpha1-3 linkage to 4-substituted GlcNAc in vitro and does not modify any 3-substituted GlcNAc to yield Lewis(a) oligosaccharides. With 8-methoxycarbonyloctyl glycoside acceptors used under standard conditions, FucT III acts exclusively on the type I and FucT VI only on the type II derivative. With lacto-N-tetraose, lacto-N-fucopentraose I, or LS-tetrasaccharide as substrates, FucT III modified the 3-substituted GlcNAc and the reducing glucose; FucT VI recognized only lacto-N-neotetraose as a substrate.
43 schema:genre research_article
44 schema:inLanguage en
45 schema:isAccessibleForFree false
46 schema:isPartOf N065eeede6cfe4761a439351e629309b0
47 N2286c1ce98544bcc809c67482bde96fb
48 sg:journal.1096094
49 schema:name In vitro α1-3 or α1-4 fucosylation of type I and II oligosaccharides with secreted forms of recombinant human fucosyltransferases III and VI
50 schema:pagination 873-883
51 schema:productId N17e9be03a8fc4f16b9947642aded2fbd
52 N485fd28d5a90461daa530349724873ae
53 N6f5c7940a60e4f899ec23e39ef1424f7
54 Ne33767627c52430aa99d7575dabf3646
55 Nfa6a30e78084460c844ef079c11b32b4
56 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014590770
57 https://doi.org/10.1023/a:1006907031940
58 schema:sdDatePublished 2019-04-10T18:17
59 schema:sdLicense https://scigraph.springernature.com/explorer/license/
60 schema:sdPublisher Nf5e582545beb409e81efc082c16fa25f
61 schema:url http://link.springer.com/10.1023/A:1006907031940
62 sgo:license sg:explorer/license/
63 sgo:sdDataset articles
64 rdf:type schema:ScholarlyArticle
65 N065eeede6cfe4761a439351e629309b0 schema:volumeNumber 15
66 rdf:type schema:PublicationVolume
67 N1014872089ab402c8584afc168d83ce5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
68 schema:name Recombinant Proteins
69 rdf:type schema:DefinedTerm
70 N17e9be03a8fc4f16b9947642aded2fbd schema:name doi
71 schema:value 10.1023/a:1006907031940
72 rdf:type schema:PropertyValue
73 N1a1bae5b1994497dbe5348b4d1e58751 rdf:first sg:person.01025224327.15
74 rdf:rest N69a08fa72c4545b0a7fd2c8632444139
75 N1cb4e57739c6460e92a97212b758a28c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
76 schema:name Trisaccharides
77 rdf:type schema:DefinedTerm
78 N2286c1ce98544bcc809c67482bde96fb schema:issueNumber 9
79 rdf:type schema:PublicationIssue
80 N236da3d43f9d49c588dd5403af714909 rdf:first sg:person.011402553405.31
81 rdf:rest N626383d819c24e4a9dfdbd50d9892e15
82 N2c690e35a79947da82a6b91f2172e40a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
83 schema:name Carbohydrate Sequence
84 rdf:type schema:DefinedTerm
85 N2e63c19e8f0c48cd999b6a4aacbbbf07 rdf:first sg:person.01313334742.81
86 rdf:rest N1a1bae5b1994497dbe5348b4d1e58751
87 N36a165f7bd2a4756a8ae9c6c46f1c93b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name Lewis Blood-Group System
89 rdf:type schema:DefinedTerm
90 N383f3ef9c92245ceba740c09b5e2f5f5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
91 schema:name Galactose
92 rdf:type schema:DefinedTerm
93 N46c66a34997445d18f323ab8a81275bf rdf:first sg:person.01216175536.16
94 rdf:rest N8c7fa331a64d42d08b04f9a15ab9a6f3
95 N485fd28d5a90461daa530349724873ae schema:name dimensions_id
96 schema:value pub.1014590770
97 rdf:type schema:PropertyValue
98 N4974f74cba4b45d098142aec48eeb6cd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Fucosyltransferases
100 rdf:type schema:DefinedTerm
101 N52f27d969e6b41c79e7d90ac9d67460d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
102 schema:name Oligosaccharides
103 rdf:type schema:DefinedTerm
104 N5519d409d7e0445f9171d72241228e4a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Transfection
106 rdf:type schema:DefinedTerm
107 N55e9dcb13d2b4ff99fde7dbd95bc3732 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
108 schema:name Cell Line
109 rdf:type schema:DefinedTerm
110 N626383d819c24e4a9dfdbd50d9892e15 rdf:first sg:person.01346153110.13
111 rdf:rest rdf:nil
112 N6937594a74d04e248f9684808b3ff279 schema:name ITQB/IBET, Apartado 12, P-2780, Oeiras, Portugal
113 rdf:type schema:Organization
114 N69a08fa72c4545b0a7fd2c8632444139 rdf:first sg:person.0622547010.34
115 rdf:rest N46c66a34997445d18f323ab8a81275bf
116 N6e5c7ab6c53d4714a96c6a760e41bf9f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Substrate Specificity
118 rdf:type schema:DefinedTerm
119 N6f5c7940a60e4f899ec23e39ef1424f7 schema:name readcube_id
120 schema:value 4582ababc0aebf255e2226e18a2fa5ae077816bfe0e8860283b3dba32b8240f5
121 rdf:type schema:PropertyValue
122 N6f9020e6a7f6484fad2b3e6eed47f455 schema:name Institut für Organische Chemie, Universität Hamburg, Martin-Luther-King Platz 6, 20146, Hamburg, Germany
123 rdf:type schema:Organization
124 N8c7fa331a64d42d08b04f9a15ab9a6f3 rdf:first sg:person.014054115613.54
125 rdf:rest N236da3d43f9d49c588dd5403af714909
126 N8ef19e8ab2094fe19e601d28a5b7df83 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Disaccharides
128 rdf:type schema:DefinedTerm
129 N9bcdc4049be945398f76571d6235abab rdf:first sg:person.01310136664.45
130 rdf:rest N2e63c19e8f0c48cd999b6a4aacbbbf07
131 Nc965b580b7b54b038d508adf12c738be schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
132 schema:name Molecular Sequence Data
133 rdf:type schema:DefinedTerm
134 Ncd1a7f4fdd674a0ba0727e054c1b6933 schema:name Institut für Organische Chemie, Universität Hamburg, Martin-Luther-King Platz 6, 20146, Hamburg, Germany
135 rdf:type schema:Organization
136 Nda7522b36e84400d9213a90d1fb11406 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Methylation
138 rdf:type schema:DefinedTerm
139 Ne33767627c52430aa99d7575dabf3646 schema:name nlm_unique_id
140 schema:value 8603310
141 rdf:type schema:PropertyValue
142 Nf55139499ab64ab9b161b088d3294ad6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Humans
144 rdf:type schema:DefinedTerm
145 Nf5e582545beb409e81efc082c16fa25f schema:name Springer Nature - SN SciGraph project
146 rdf:type schema:Organization
147 Nfa6a30e78084460c844ef079c11b32b4 schema:name pubmed_id
148 schema:value 10052591
149 rdf:type schema:PropertyValue
150 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
151 schema:name Medical and Health Sciences
152 rdf:type schema:DefinedTerm
153 anzsrc-for:1115 schema:inDefinedTermSet anzsrc-for:
154 schema:name Pharmacology and Pharmaceutical Sciences
155 rdf:type schema:DefinedTerm
156 sg:journal.1096094 schema:issn 0969-3653
157 1573-4986
158 schema:name Glycoconjugate Journal
159 rdf:type schema:Periodical
160 sg:person.01025224327.15 schema:affiliation N6f9020e6a7f6484fad2b3e6eed47f455
161 schema:familyName Gambert
162 schema:givenName Ulrike
163 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01025224327.15
164 rdf:type schema:Person
165 sg:person.011402553405.31 schema:affiliation Ncd1a7f4fdd674a0ba0727e054c1b6933
166 schema:familyName Thiem
167 schema:givenName Joachim
168 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011402553405.31
169 rdf:type schema:Person
170 sg:person.01216175536.16 schema:affiliation https://www.grid.ac/institutes/grid.418123.d
171 schema:familyName Wray
172 schema:givenName Victor
173 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01216175536.16
174 rdf:type schema:Person
175 sg:person.01310136664.45 schema:affiliation https://www.grid.ac/institutes/grid.418123.d
176 schema:familyName Nimtz
177 schema:givenName Manfred
178 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01310136664.45
179 rdf:type schema:Person
180 sg:person.01313334742.81 schema:affiliation https://www.grid.ac/institutes/grid.418123.d
181 schema:familyName Grabenhorst
182 schema:givenName Eckart
183 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01313334742.81
184 rdf:type schema:Person
185 sg:person.01346153110.13 schema:affiliation https://www.grid.ac/institutes/grid.418123.d
186 schema:familyName Conradt
187 schema:givenName Harald S.
188 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01346153110.13
189 rdf:type schema:Person
190 sg:person.014054115613.54 schema:affiliation https://www.grid.ac/institutes/grid.418123.d
191 schema:familyName Morr
192 schema:givenName Michael
193 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014054115613.54
194 rdf:type schema:Person
195 sg:person.0622547010.34 schema:affiliation N6937594a74d04e248f9684808b3ff279
196 schema:familyName Costa
197 schema:givenName Julia
198 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0622547010.34
199 rdf:type schema:Person
200 sg:pub.10.1007/bf00731136 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007244866
201 https://doi.org/10.1007/bf00731136
202 rdf:type schema:CreativeWork
203 sg:pub.10.1007/bf00731137 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000547172
204 https://doi.org/10.1007/bf00731137
205 rdf:type schema:CreativeWork
206 https://app.dimensions.ai/details/publication/pub.1075699150 schema:CreativeWork
207 https://doi.org/10.1016/0006-291x(91)91875-d schema:sameAs https://app.dimensions.ai/details/publication/pub.1052172532
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1016/0006-291x(92)90697-j schema:sameAs https://app.dimensions.ai/details/publication/pub.1021977142
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1016/0014-5793(90)80361-l schema:sameAs https://app.dimensions.ai/details/publication/pub.1006175409
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1016/0092-8674(90)90444-j schema:sameAs https://app.dimensions.ai/details/publication/pub.1037836709
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1016/s0006-291x(05)81472-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1053619695
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1016/s0008-6215(96)00324-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013536924
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1016/s0014-5793(97)01121-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035053328
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1021/bi00199a016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055164907
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1021/bi00448a031 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055176669
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1073/pnas.91.16.7390 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034813465
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1074/jbc.270.15.8712 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004916630
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1074/jbc.272.17.11613 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032304279
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1083/jcb.117.4.895 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052019930
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1093/glycob/7.7.921 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020337535
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1093/oxfordjournals.jbchem.a127869 schema:sameAs https://app.dimensions.ai/details/publication/pub.1076282982
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1101/gad.4.8.1288 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002160202
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1111/j.1432-1033.1993.tb17732.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1005024080
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1111/j.1432-1033.1993.tb18042.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1026688297
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1111/j.1432-1033.1995.718zz.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1005259043
244 rdf:type schema:CreativeWork
245 https://www.grid.ac/institutes/grid.418123.d schema:alternateName GBF German Research Centre for Biotechnology
246 schema:name Gesellschaft für Biotechnologische Forschung, Mascheroder Weg 1, 38124, Braunschweig, Germany
247 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...