Effect of dexamethasone on T-cell receptor/CD3 expression View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1997-02

AUTHORS

Graziella Migliorati, Andrea Bartoli, Giuseppe Nocentini, Simona Ronchetti, Rosalba Moraca, Carlo Riccardi

ABSTRACT

Glucocorticoid hormones (GCH) are anti-inflammatory and immunosuppressive agents that inhibit T-cell growth and activation. Since the T-cell receptor (TCR)/CD3 complex mediates T-lymphocyte activation, we studied the effect of in vitro dexamethasone (DEX), a synthetic GCH, on TCR/CD3 expression. DEX-treatment of a hybridoma T-cell line and normal un-transformed T-cell clones induced a decrease of the TCR/ CD3 membrane expression after 4 days. After 4 weeks, TCR/CD3 was undetectable. However, the amount of mRNAs coding TCR/CD3 chains, including TCR alpha, TCR beta, CD3 gamma, CD3 theta and CD3 epsilon, as well as the amount of CD3 epsilon protein, a major component of the complex, were unaltered. By contrast, a decrease of the mRNAs deriving from the TCR zeta gene locus, as well as of the TCR zeta protein which is responsible for the membrane expression of the TCR/CD3 complex, was induced. These data suggest that the down-modulation of TCR expression is due to the diminution of TCR zeta gene products in DEX-treated cells. More... »

PAGES

135-144

References to SciGraph publications

  • 1986-01. Multiple control of inflammation by glucocorticoids in INFLAMMATION RESEARCH
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1023/a:1006829421509

    DOI

    http://dx.doi.org/10.1023/a:1006829421509

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/9059990


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