Metastasis-suppressed C8161 melanoma cells arrest in lung but fail to proliferate View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1999-07

AUTHORS

Steven F. Goldberg, John F. Harms, Kim Quon, Danny R. Welch

ABSTRACT

The incidence of melanoma continues to increase at a rapid rate. As for most cancers, it is melanoma metastases, rather than the primary malignancy, that is the principal cause of death. We previously showed that the introduction of a normal copy of chromosome 6 into the metastatic human melanoma cell line C8161 suppresses metastasis at a step subsequent to tumor cells entering the bloodstream. To better define the step(s) in metastasis blocked by the addition of chromosome 6 we engineered cells that constitutively express green fluorescent protein (GFP). When these tagged, chromosome 6 hybrid cells were injected intravenously into athymic mice, grossly detectable metastases did not form. However, fluorescence microscopy revealed micro-metastases (single cells or clusters of <10 cells) in the lungs, suggesting that these cells lodged in the lungs but failed to proliferate. Cells isolated from lung up to 60 days post-injection grew in culture and/or formed tumors when injected into the skin, indicating that they were still viable, but dormant. This result implies that the gene(s) on chromosome 6 interfere specifically with growth regulatory response in the lung, but not in the skin. Thus, the gene(s) responsible for metastasis suppression represents a new class of metastasis inhibitors acting at the final stages of the metastatic cascade--that is, affecting the ability of the cells to survive and proliferate at a specific secondary site. More... »

PAGES

601-607

References to SciGraph publications

  • 1997-05. Technical considerations for studying cancer metastasis in vivo in CLINICAL & EXPERIMENTAL METASTASIS
  • 1988. The cytogenetics of human malignant melanoma and premalignant lesions in MALIGNANT MELANOMA: BIOLOGY, DIAGNOSIS, AND THERAPY
  • 1998-09. Orthotopic Models are Necessary to Predict Therapy of Transplantable Tumors in Mice in CANCER AND METASTASIS REVIEWS
  • 1998-11. Prognostic significance of micrometastatic bone marrow involvement in BREAST CANCER RESEARCH AND TREATMENT
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1023/a:1006718800891

    DOI

    http://dx.doi.org/10.1023/a:1006718800891

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/10845559


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