pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-04

AUTHORS

Roger P. Hellens, E. Anne Edwards, Nicola R. Leyland, Samantha Bean, Philip M. Mullineaux

ABSTRACT

Binary Ti vectors are the plasmid vectors of choice in Agrobacterium-mediated plant transformation protocols. The pGreen series of binary Ti vectors are configured for ease-of-use and to meet the demands of a wide range of transformation procedures for many plant species. This plasmid system allows any arrangement of selectable marker and reporter gene at the right and left T-DNA borders without compromising the choice of restriction sites for cloning, since the pGreen cloning sites are based on the well-known pBluescript general vector plasmids. Its size and copy number in Escherichia coli offers increased efficiencies in routine in vitro recombination procedures. pGreen can replicate in Agrobacterium only if another plasmid, pSoup, is co-resident in the same strain. pSoup provides replication functions in trans for pGreen. The removal of RepA and Mob functions has enabled the size of pGreen to be kept to a minimum. Versions of pGreen have been used to transform several plant species with the same efficiencies as other binary Ti vectors. Information on the pGreen plasmid system is supplemented by an Internet site (http://www.pgreen.ac.uk) through which comprehensive information, protocols, order forms and lists of different pGreen marker gene permutations can be found. More... »

PAGES

819-832

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1023/a:1006496308160

    DOI

    http://dx.doi.org/10.1023/a:1006496308160

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1041909606

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/10890530


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