Plant regeneration from embryogenic cultures of Phragmites australis (Cav.) Trin. Ex Steud. View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-10

AUTHORS

Csaba Máthé, Márta M. Hamvas, István Grigorszky, Gábor Vasas, Erika Molnár, J. Brian Power, Michael R. Davey, George Borbély

ABSTRACT

Embryogenic cultures of the common reed [Phragmites australis (Cav.) Trin. Ex. Steud.] were induced on Murashige and Skoog (1962)-based medium with 2% (w/v) sucrose, B5 vitamins and 4.5 μM 2,4-dichlorphenoxyacetic acid. Four independent culture lines, two initiated from stem nodes and two from roots, were established. These cultures underwent somatic embryogenesis. In one line of stem node origin, the somatic embryos germinated and developed into plants, following transfer of embryogenic cultures to Murashige and Skoog (1962)-based medium lacking growth regulators, with 108 ± 17 plants being recovered per 100 mg fresh weight of culture. In other lines, the somatic embryos developed roots, but not shoots. Shoot regeneration via somatic embryogenesis offers potential as anin vitro system for physiological studies, including assessments of the response of common reed to environmental pollutants. More... »

PAGES

81-84

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1006471515103

DOI

http://dx.doi.org/10.1023/a:1006471515103

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1014543689


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140 https://www.grid.ac/institutes/grid.7122.6 schema:alternateName University of Debrecen
141 schema:name Department of Botany, Kossuth University of Debrecen, 4010-Debrecen, P.O. Box 14, Hungary
142 rdf:type schema:Organization
 




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