Plant regeneration from protoplasts of a commercial Asian long-grain javanica rice View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-01

AUTHORS

K. Tang, X. Sun, D. An, J.B. Power, E.C. Cocking, M.R. Davey

ABSTRACT

A reproducible plant regeneration system has been developed for protoplasts from embryogenic cell suspension cultures of the commercial Asian long-grain javanica rice, Oryza sativa cv. Azucena. Protoplasts were isolated routinely from cell suspensions with yields of 5.5–12.0 × 106 g-1 fresh weight. A membrane filter nurse-culture method was adopted and was essential to support sustained mitotic division of protoplast-derived cells, leading to cell colony formation. The protoplast plating efficiency was higher when suspension cells of Lolium multiflorum, rather than those of the japonica rice O. sativa L. cv. Taipei 309, were employed as nurse cells. A two-step shoot regeneration procedure, in which protoplast-derived calli were cultured initially on medium semi-solidified with 1% (w/v) agarose followed by culture on medium containing 0.4% (w/v) agarose, induced plant regeneration from protoplast-derived calli. Fifteen percent of protoplast-derived tissues regenerated shoots; tissues not subjected to this treatment failed to develop shoots. More... »

PAGES

79-82

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1006408109595

DOI

http://dx.doi.org/10.1023/a:1006408109595

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1033929289


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