Microspore-derived embryo induction from cultured anthers of wheat View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-03

AUTHORS

H.M. Ingram, J.B. Power, K.C. Lowe, M.R. Davey

ABSTRACT

Microspore-derived embryo (MDE) induction was evaluated following the culture of wheat (Triticum aestivum L.) anthers in a basal medium with a range of supplements. Several supplements were evaluated, including a commercial bovine haemoglobin solution (Erythrogen™), Ficoll and the co-polymer surfactant Pluronic F-68, but these did not enhance MDE induction. However, phenylacetic acid, replacing both 2,4-dichlorophenoxyacetic acid and kinetin, increased MDE induction by a factor of 1.6 (p<0.05), producing 257±29 (mean±s.e., n=3) MDEs per 100 anthers cultured. Twenty-four plants regenerated from MDEs induced via anther culture were used for ploidy determinations; 33% were sterile haploids, while the remainder were fertile. More... »

PAGES

235-238

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1006400915551

DOI

http://dx.doi.org/10.1023/a:1006400915551

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1051758800


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