In vitro shoot regeneration from leaves of wild pear View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1999-10

AUTHORS

E. Caboni, M.G. Tonelli, P. Lauri, S. D'Angeli, C. Damiano

ABSTRACT

Shoot regeneration was obtained from leaves of in vitro cultures of wild pear genotypes. The highest regeneration rates, ranging from 40% to 64%, depending on the genotype, were obtained using leaves wounded by three cuts transversely to the mid-rib, a Quoirin and Lepoivre macro-salt composition, 250 mg l-1 cefotaxime and maintaining the explants in darkness for the first 30 days (induction phase), then transferring them to an auxin-free medium in light (expression phase). A concentration of 8.8 μM BA induced the highest number of explants to produce adventitious shoots. TDZ was less effective than BA and induced hyperhydricity in regenerated shoots. The histological studies revealed that the regenerated shoots originated mainly from callus formed by epidermal and sub-epidermal cells and by cells of the vascular tissue. The regenerated shoots were micropropagated, rooted and transplanted to the soil. More... »

PAGES

1-7

References to SciGraph publications

  • 1994-07. Micropropagation of thirteen Malus cultivars and rootstocks, and effect of antibiotics on proliferation in PLANT GROWTH REGULATION
  • 1990-08. Direct shoot organogenesis and plant regeneration from leaves of grape (Vitis spp.) in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1989-01. Adventitions shoot formation on excised leaves of in vitro grown shoots of apple cultivars in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1990-05. The effect of in vitro pretreatments on subsequent shoot organogenesis from excised Rubus and Malus leaves in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1991-05. Adventitious shoot regeneration from in vitro leaves of several pear cultivars (Pyrus communis L.) in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1989-03. Adventitious shoot regeneration from leaf tissue of three pear (Pyrus sp.) cultivars in vitro in PLANT CELL REPORTS
  • 1994-06. Factors that effect leaf regeneration efficiency in apple, and effect of antibiotics in morphogenesis in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1996-07. Shoot regeneration from adventitious buds induced on juvenile and adult almond (Prunus dulcis mill.) explants in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - PLANT
  • 1998-07. Shoot regeneration from cultured leaves of Japanese pear (Pyrus pyrifolia) in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1993-01. Enhanced plant regeneration in pearl millet (Pennisetum americanum) by ethylene inhibitors and cefotaxime in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1991-12. Influence of nitrogen form and NH4+-N/:NO3−-N ratios on adventitious shoot formation from pear (Pyrus communis) leaf explants in vitro in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1023/a:1006351214343

    DOI

    http://dx.doi.org/10.1023/a:1006351214343

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1035106928


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