Somatic embryogenesis of Panax ginseng in liquid cultures: a role for polyamines and their metabolic pathways View Full Text


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Article Info

DATE

2000-07

AUTHORS

Claire Kevers, Nathalie Le Gal, Marta Monteiro, Jacques Dommes, Thomas Gaspar

ABSTRACT

A callus with embryogenic capacity was generated fromroot sections of Panax ginseng and used as aninoculum source for embryogenic liquid cultures in athree-step process: – a suspension culture of cellaggregates in the presence of an auxin/cytokininmixture, – an induction medium containing auxin only(for 5 to 30 days), – a regeneration medium containingcytokinin only (for one month). Up to 25 embryos wererecovered per 2.5 g of aggregates in these conditions.Incorporation of polyamines or their precursorsarginine and ornithine into either the induction orregeneration media increased the number of embryosproduced by up to 4 times. Inhibitors of bothbiosynthesis and biodegradation of polyamines reducedthe number of embryos. These results support earlierfindings of the role of polyamines in the process ofsomatic embryogenesis. The success of these liquidcultures opens up the possibility of producing somaticembryos of Panax ginseng in bioreactors. More... »

PAGES

209-214

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1006344316683

DOI

http://dx.doi.org/10.1023/a:1006344316683

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1038430606


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