In vitro root cultures of Panax ginseng and P. quinquefolium View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1999-03

AUTHORS

C. Kevers, Ph. Jacques, Ph. Thonart, Th. Gaspar

ABSTRACT

The paper describes a procedure for the initiation, subculture and continued proliferation of adventitious roots of Panax ginseng and Panax quinquefolium, which resemble hairy roots. The technique took advantage of the high powerful activity of a new synthetic auxin: benzo[b]selenienyl acetic acid (BSAA). Such initiation from root explants was dependent upon the season, the type and concentration of auxin. The hairy-like roots of ginseng could be subcultured by transfer every 4 weeks to fresh liquid medium either in agitated Erlenmeyer flasks or in bioreactors. Optimal conditions for a continued multiplication (up to 14 per month) were determined. The only practical problem was the limitation of the fresh mass as inoculum: the multiplication rate decreased with the increased quantity of roots. It is postulated that a root growth inhibiting substance was released into the media by the proliferating ginseng hairy roots. More... »

PAGES

173-179

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1006266413919

DOI

http://dx.doi.org/10.1023/a:1006266413919

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1032712528


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0607", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Plant Biology", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Institute of Botany B 22, University of Li\u00e8ge, Sart Tilman, B-4000, Li\u00e8ge, Belgium (corresponding author: phone and fax", 
          "id": "http://www.grid.ac/institutes/grid.4861.b", 
          "name": [
            "Institute of Botany B 22, University of Li\u00e8ge, Sart Tilman, B-4000, Li\u00e8ge, Belgium (corresponding author: phone and fax"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kevers", 
        "givenName": "C.", 
        "id": "sg:person.01117022246.80", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01117022246.80"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "CWBI, B 40, University of Li\u00e8ge, Sart Tilman, B-4000, Li\u00e8ge, Belgium", 
          "id": "http://www.grid.ac/institutes/grid.4861.b", 
          "name": [
            "CWBI, B 40, University of Li\u00e8ge, Sart Tilman, B-4000, Li\u00e8ge, Belgium"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jacques", 
        "givenName": "Ph.", 
        "id": "sg:person.01207433132.20", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01207433132.20"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "CWBI, B 40, University of Li\u00e8ge, Sart Tilman, B-4000, Li\u00e8ge, Belgium", 
          "id": "http://www.grid.ac/institutes/grid.4861.b", 
          "name": [
            "CWBI, B 40, University of Li\u00e8ge, Sart Tilman, B-4000, Li\u00e8ge, Belgium"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Thonart", 
        "givenName": "Ph.", 
        "id": "sg:person.01343267150.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01343267150.07"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Botany B 22, University of Li\u00e8ge, Sart Tilman, B-4000, Li\u00e8ge, Belgium (corresponding author: phone and fax", 
          "id": "http://www.grid.ac/institutes/grid.4861.b", 
          "name": [
            "Institute of Botany B 22, University of Li\u00e8ge, Sart Tilman, B-4000, Li\u00e8ge, Belgium (corresponding author: phone and fax"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gaspar", 
        "givenName": "Th.", 
        "id": "sg:person.07736002024.11", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07736002024.11"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf02822700", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037386254", 
          "https://doi.org/10.1007/bf02822700"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00272780", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1078815004", 
          "https://doi.org/10.1007/bf00272780"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02866780", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023232091", 
          "https://doi.org/10.1007/bf02866780"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00231935", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004504798", 
          "https://doi.org/10.1007/bf00231935"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-4615-5403-5_70", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048662486", 
          "https://doi.org/10.1007/978-1-4615-5403-5_70"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00232452", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015390456", 
          "https://doi.org/10.1007/bf00232452"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1999-03", 
    "datePublishedReg": "1999-03-01", 
    "description": "The paper describes a procedure for the initiation, subculture and continued proliferation of adventitious roots of Panax ginseng and Panax quinquefolium, which resemble hairy roots. The technique took advantage of the high powerful activity of a new synthetic auxin: benzo[b]selenienyl acetic acid (BSAA). Such initiation from root explants was dependent upon the season, the type and concentration of auxin. The hairy-like roots of ginseng could be subcultured by transfer every 4 weeks to fresh liquid medium either in agitated Erlenmeyer flasks or in bioreactors. Optimal conditions for a continued multiplication (up to 14 per month) were determined. The only practical problem was the limitation of the fresh mass as inoculum: the multiplication rate decreased with the increased quantity of roots. It is postulated that a root growth inhibiting substance was released into the media by the proliferating ginseng hairy roots.", 
    "genre": "article", 
    "id": "sg:pub.10.1023/a:1006266413919", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1130569", 
        "issn": [
          "0167-6903", 
          "1573-5087"
        ], 
        "name": "Plant Growth Regulation", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "3", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "27"
      }
    ], 
    "keywords": [
      "fresh liquid", 
      "bioreactor", 
      "optimal conditions", 
      "acetic acid", 
      "transfer", 
      "liquid", 
      "Erlenmeyer flasks", 
      "practical problems", 
      "initiation", 
      "continued proliferation", 
      "Panax ginseng", 
      "technique", 
      "advantages", 
      "concentration", 
      "flask", 
      "conditions", 
      "problem", 
      "limitations", 
      "rate", 
      "quantity", 
      "medium", 
      "procedure", 
      "proliferation", 
      "roots", 
      "ginseng", 
      "Panax quinquefolium", 
      "powerful activity", 
      "activity", 
      "acid", 
      "such initiation", 
      "explants", 
      "types", 
      "weeks", 
      "multiplication", 
      "mass", 
      "inoculum", 
      "quantity of roots", 
      "growth", 
      "substances", 
      "subculture", 
      "quinquefolium", 
      "season", 
      "fresh mass", 
      "multiplication rate", 
      "root growth", 
      "culture", 
      "paper", 
      "adventitious roots", 
      "hairy roots", 
      "synthetic auxin", 
      "auxin", 
      "root explants", 
      "concentration of auxin", 
      "ginseng hairy roots", 
      "vitro root cultures", 
      "root cultures", 
      "high powerful activity", 
      "new synthetic auxin", 
      "hairy-like roots", 
      "only practical problem"
    ], 
    "name": "In vitro root cultures of Panax ginseng and P. quinquefolium", 
    "pagination": "173-179", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1032712528"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1023/a:1006266413919"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1023/a:1006266413919", 
      "https://app.dimensions.ai/details/publication/pub.1032712528"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2021-11-01T18:04", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20211101/entities/gbq_results/article/article_334.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1023/a:1006266413919"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1023/a:1006266413919'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1023/a:1006266413919'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1023/a:1006266413919'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1023/a:1006266413919'


 

This table displays all metadata directly associated to this object as RDF triples.

165 TRIPLES      22 PREDICATES      92 URIs      78 LITERALS      6 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1023/a:1006266413919 schema:about anzsrc-for:06
2 anzsrc-for:0607
3 schema:author N89ea25e34f464b90a0704edbe5321929
4 schema:citation sg:pub.10.1007/978-1-4615-5403-5_70
5 sg:pub.10.1007/bf00231935
6 sg:pub.10.1007/bf00232452
7 sg:pub.10.1007/bf00272780
8 sg:pub.10.1007/bf02822700
9 sg:pub.10.1007/bf02866780
10 schema:datePublished 1999-03
11 schema:datePublishedReg 1999-03-01
12 schema:description The paper describes a procedure for the initiation, subculture and continued proliferation of adventitious roots of Panax ginseng and Panax quinquefolium, which resemble hairy roots. The technique took advantage of the high powerful activity of a new synthetic auxin: benzo[b]selenienyl acetic acid (BSAA). Such initiation from root explants was dependent upon the season, the type and concentration of auxin. The hairy-like roots of ginseng could be subcultured by transfer every 4 weeks to fresh liquid medium either in agitated Erlenmeyer flasks or in bioreactors. Optimal conditions for a continued multiplication (up to 14 per month) were determined. The only practical problem was the limitation of the fresh mass as inoculum: the multiplication rate decreased with the increased quantity of roots. It is postulated that a root growth inhibiting substance was released into the media by the proliferating ginseng hairy roots.
13 schema:genre article
14 schema:inLanguage en
15 schema:isAccessibleForFree false
16 schema:isPartOf N531cdcfc148a4302a1c0cd980cc9763a
17 Nfaa94b8af3714cc09ad9ee020afad600
18 sg:journal.1130569
19 schema:keywords Erlenmeyer flasks
20 Panax ginseng
21 Panax quinquefolium
22 acetic acid
23 acid
24 activity
25 advantages
26 adventitious roots
27 auxin
28 bioreactor
29 concentration
30 concentration of auxin
31 conditions
32 continued proliferation
33 culture
34 explants
35 flask
36 fresh liquid
37 fresh mass
38 ginseng
39 ginseng hairy roots
40 growth
41 hairy roots
42 hairy-like roots
43 high powerful activity
44 initiation
45 inoculum
46 limitations
47 liquid
48 mass
49 medium
50 multiplication
51 multiplication rate
52 new synthetic auxin
53 only practical problem
54 optimal conditions
55 paper
56 powerful activity
57 practical problems
58 problem
59 procedure
60 proliferation
61 quantity
62 quantity of roots
63 quinquefolium
64 rate
65 root cultures
66 root explants
67 root growth
68 roots
69 season
70 subculture
71 substances
72 such initiation
73 synthetic auxin
74 technique
75 transfer
76 types
77 vitro root cultures
78 weeks
79 schema:name In vitro root cultures of Panax ginseng and P. quinquefolium
80 schema:pagination 173-179
81 schema:productId Nabd9de65868d4fe1acad4eae09ab32f4
82 Nd9b46628c6044e8095ff17d4204ae7d3
83 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032712528
84 https://doi.org/10.1023/a:1006266413919
85 schema:sdDatePublished 2021-11-01T18:04
86 schema:sdLicense https://scigraph.springernature.com/explorer/license/
87 schema:sdPublisher Ndf21133d4c8947d5bde57089ecadc29f
88 schema:url https://doi.org/10.1023/a:1006266413919
89 sgo:license sg:explorer/license/
90 sgo:sdDataset articles
91 rdf:type schema:ScholarlyArticle
92 N3a149bff38cd4ab59147082f67dda613 rdf:first sg:person.07736002024.11
93 rdf:rest rdf:nil
94 N531cdcfc148a4302a1c0cd980cc9763a schema:volumeNumber 27
95 rdf:type schema:PublicationVolume
96 N89ea25e34f464b90a0704edbe5321929 rdf:first sg:person.01117022246.80
97 rdf:rest N9513956f0af6472cbd49c1047e7af247
98 N9513956f0af6472cbd49c1047e7af247 rdf:first sg:person.01207433132.20
99 rdf:rest Nd27f4734bbf047d48a08cd41f8ed228f
100 Nabd9de65868d4fe1acad4eae09ab32f4 schema:name dimensions_id
101 schema:value pub.1032712528
102 rdf:type schema:PropertyValue
103 Nd27f4734bbf047d48a08cd41f8ed228f rdf:first sg:person.01343267150.07
104 rdf:rest N3a149bff38cd4ab59147082f67dda613
105 Nd9b46628c6044e8095ff17d4204ae7d3 schema:name doi
106 schema:value 10.1023/a:1006266413919
107 rdf:type schema:PropertyValue
108 Ndf21133d4c8947d5bde57089ecadc29f schema:name Springer Nature - SN SciGraph project
109 rdf:type schema:Organization
110 Nfaa94b8af3714cc09ad9ee020afad600 schema:issueNumber 3
111 rdf:type schema:PublicationIssue
112 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
113 schema:name Biological Sciences
114 rdf:type schema:DefinedTerm
115 anzsrc-for:0607 schema:inDefinedTermSet anzsrc-for:
116 schema:name Plant Biology
117 rdf:type schema:DefinedTerm
118 sg:journal.1130569 schema:issn 0167-6903
119 1573-5087
120 schema:name Plant Growth Regulation
121 schema:publisher Springer Nature
122 rdf:type schema:Periodical
123 sg:person.01117022246.80 schema:affiliation grid-institutes:grid.4861.b
124 schema:familyName Kevers
125 schema:givenName C.
126 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01117022246.80
127 rdf:type schema:Person
128 sg:person.01207433132.20 schema:affiliation grid-institutes:grid.4861.b
129 schema:familyName Jacques
130 schema:givenName Ph.
131 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01207433132.20
132 rdf:type schema:Person
133 sg:person.01343267150.07 schema:affiliation grid-institutes:grid.4861.b
134 schema:familyName Thonart
135 schema:givenName Ph.
136 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01343267150.07
137 rdf:type schema:Person
138 sg:person.07736002024.11 schema:affiliation grid-institutes:grid.4861.b
139 schema:familyName Gaspar
140 schema:givenName Th.
141 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07736002024.11
142 rdf:type schema:Person
143 sg:pub.10.1007/978-1-4615-5403-5_70 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048662486
144 https://doi.org/10.1007/978-1-4615-5403-5_70
145 rdf:type schema:CreativeWork
146 sg:pub.10.1007/bf00231935 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004504798
147 https://doi.org/10.1007/bf00231935
148 rdf:type schema:CreativeWork
149 sg:pub.10.1007/bf00232452 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015390456
150 https://doi.org/10.1007/bf00232452
151 rdf:type schema:CreativeWork
152 sg:pub.10.1007/bf00272780 schema:sameAs https://app.dimensions.ai/details/publication/pub.1078815004
153 https://doi.org/10.1007/bf00272780
154 rdf:type schema:CreativeWork
155 sg:pub.10.1007/bf02822700 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037386254
156 https://doi.org/10.1007/bf02822700
157 rdf:type schema:CreativeWork
158 sg:pub.10.1007/bf02866780 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023232091
159 https://doi.org/10.1007/bf02866780
160 rdf:type schema:CreativeWork
161 grid-institutes:grid.4861.b schema:alternateName CWBI, B 40, University of Liège, Sart Tilman, B-4000, Liège, Belgium
162 Institute of Botany B 22, University of Liège, Sart Tilman, B-4000, Liège, Belgium (corresponding author: phone and fax
163 schema:name CWBI, B 40, University of Liège, Sart Tilman, B-4000, Liège, Belgium
164 Institute of Botany B 22, University of Liège, Sart Tilman, B-4000, Liège, Belgium (corresponding author: phone and fax
165 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...