Semi-quantitative RT-PCR analysis of photoregulated gene expression in marine diatoms View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1999-08

AUTHORS

Catherine Leblanc, Angela Falciatore, Masakatsu Watanabe, Chris Bowler

ABSTRACT

The low cell densities of diatoms and other phytoplankton in culture has precluded the use of classical RNA analysis techniques for routine studies of gene expression in large numbers of samples. This has seriously hampered studies of the basic biology of such organisms. To circumvent this problem, we have developed a high-throughput semi-quantitative RT-PCR-based protocol and used it to monitor expression of a gene encoding a fucoxanthin, chlorophyll a/c-binding protein (FCP) in the centric planktonic diatom Thalassiosira weissflogii. Analysis of FCP gene expression in dark-adapted diatom cultures revealed that mRNA levels increase 5- to 6-fold in response to white light irradiation and peak around 6 to 8 h. To determine the photoreceptors involved in this response action spectra of FCP gene expression were determined using the Okazaki large spectrograph. Responses consistent with the presence of cryptochrome-, rhodopsin- and phytochrome-type receptors could be detected. The apparent presence of phytochrome-mediated responses is of particular interest given the low fluences of red and far-red light wavelengths in the marine environment. More... »

PAGES

1031-1044

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1023/a:1006256300969

    DOI

    http://dx.doi.org/10.1023/a:1006256300969

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1027438888

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/10527427


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