Identification and characterization of T3/T7 bacteriophage-like RNA polymerase sequences in wheat View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1999-07

AUTHORS

Tatsuya M. Ikeda, Michael W. Gray

ABSTRACT

Using PCR-based methods, we assembled two wheat cDNA sequences, wheat-G and wheat-C, that encode T3/T7 bacteriophage-like RNA polymerases (RNAPs) sharing 45% amino acid identity. In phylogenetic analyses using maximum likelihood, parsimony and distance methods, the predicted protein sequence of wheat-G (1005 amino acids, 113 kDa) clusters with sequences of previously assigned mitochondrial RNAPs from dicotyledonous plants (Arabidopsis thaliana, Chenopodium album); likewise, in such analyses, the wheat-C sequence (949 amino acids, 107 kDa) affiliates specifically with the Arabidopsis sequence that encodes a phage-like RNAP thought to function in chloroplasts. To confirm biochemically the assignment of the gene encoding the putative wheat mitochondrial RNAP, we isolated a ca. 100 kDa wheat mitochondrial protein that is enriched in fractions displaying specific in vitro transcription activity and that reacts with an antibody raised against a recombinant maize phage-type RNAP. Internal peptide sequence information obtained from the 100-kDa polypeptide revealed that it corresponds to the predicted wheat-G cDNA sequence, providing direct evidence that the wheat-G gene (which we propose to call RpoTm) encodes the wheat mitochondrial RNAP. More... »

PAGES

567-578

References to SciGraph publications

  • 1998-10. Characterization of a gene encoding a single-subunit bacteriophage-type RNA polymerase from maize which is alternatively spliced in MOLECULAR GENETICS AND GENOMICS
  • 1997-12. On the Evolution of the Single-Subunit RNA Polymerases in JOURNAL OF MOLECULAR EVOLUTION
  • 1970-08. Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 in NATURE
  • 1997-03. Protein import into plant mitochondria in PLANT MOLECULAR BIOLOGY
  • 1999. Phage-Type RNA Polymerases in Higher Plants in THE CHLOROPLAST: FROM MOLECULAR BIOLOGY TO BIOTECHNOLOGY
  • 1983-09. A plant DNA minipreparation: Version II in PLANT MOLECULAR BIOLOGY REPORTER
  • Identifiers

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    http://scigraph.springernature.com/pub.10.1023/a:1006203928189

    DOI

    http://dx.doi.org/10.1023/a:1006203928189

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/10480381


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