In vitro culture of coffee zygotic embryos for germplasm preservation View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-12

AUTHORS

M. Madhava Naidu, H.L. Sreenath

ABSTRACT

Immature zygotic embryos of Coffea arabica L. Cv. Cauvery (Catimor) were cultured on Murashige and Skoog (1962) (MS) medium supplemented with abscisic acid (ABA) at 0, 0.4, 3.8, 18.9, 37.8 and 75.6 μM., L-cystein hydrochloride at 50 mg 1-1 and sucrose at 3%. Cultures were preserved in parafilm sealed Petri dishes in dark at a temperature of 25 ± 1 °C for up to two years. The preserved embryos were taken out from the media at 6 month intervals in order to test their viability by germination on MS + NAA (0.5 μM) + BA (4.4 μM). On the preservation media devoid of ABA or with a low concentration (0.4 μM) of ABA, the embryos germinated and showed higher mortality with increasing duration of storage. In contrast, the embryos became increasingly dormant with increasing concentrations of ABA and a 74.2% survival was found even after 2 years on medium supplemented with 18.9 μM or 37.8 μM of ABA. The results suggest that embryos can be preserved with a little loss of viability in the presence of ABA even at the normal room temperature (25 + 1 °C) up to two years without any transfer. Application of this technique for germplasm preservation of coffee is discussed. More... »

PAGES

227-230

References to SciGraph publications

  • 1991-12. Minimal growth in vitro conservation of coffee (Coffea spp.) in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • Identifiers

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    http://scigraph.springernature.com/pub.10.1023/a:1006194405702

    DOI

    http://dx.doi.org/10.1023/a:1006194405702

    DIMENSIONS

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