High-efficiency Agrobacterium-mediated transformation of Norway spruce (Picea abies) and loblolly pine (Pinus taeda) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1999-02

AUTHORS

Allan Richard Wenck, Michelle Quinn, Ross W. Whetten, Gerald Pullman, Ronald Sederoff

ABSTRACT

Agrobacterium-mediated gene transfer is the method of choice for many plant biotechnology laboratories; however, large-scale use of this organism in conifer transformation has been limited by difficult propagation of explant material, selection efficiencies and low transformation frequency. We have analyzed co-cultivation conditions and different disarmed strains of Agrobacterium to improve transformation. Additional copies of virulence genes were added to three common disarmed strains. These extra virulence genes included either a constitutively active virG or extra copies of virG and virB, both from pTiBo542. In experiments with Norway spruce, we increased transformation efficiencies 1000-fold from initial experiments where little or no transient expression was detected. Over 100 transformed lines expressing the marker gene beta-glucuronidase (GUS) were generated from rapidly dividing embryogenic suspension-cultured cells co-cultivated with Agrobacterium. GUS activity was used to monitor transient expression and to further test lines selected on kanamycin-containing medium. In loblolly pine, transient expression increased 10-fold utilizing modified Agrobacterium strains. Agrobacterium-mediated gene transfer is a useful technique for large-scale generation of transgenic Norway spruce and may prove useful for other conifer species. More... »

PAGES

407-416

References to SciGraph publications

  • 1992-09. Genetic transformation of Norway spruce (Picea abies (L.) Karst) using somatic embryo explants by microprojectile bombardment in PLANT MOLECULAR BIOLOGY
  • 1991-10. Agrobacterium rhizogenes-mediated genetic transformation and regeneration of a conifer:Larix decidua in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - PLANT
  • 1988. Binary vectors in PLANT MOLECULAR BIOLOGY MANUAL
  • 1992-10. Characterization of competent cells and early events of Agrobacterium-mediated genetic transformation in Arabidopsis thaliana in PLANTA
  • 1995. Somatic Embryogenesis in Black Spruce [Picea mariana (Mill.) B.S.P.] and Red Spruce (P. rubens Sarg.) in SOMATIC EMBRYOGENESIS AND SYNTHETIC SEED I
  • 1997-06. Role of the host cell cycle in theAgrobacterium-mediated genetic transformation ofPetunia: Evidence of an S-phase control mechanism for T-DNA transfer in PLANTA
  • 1983-05. A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid in NATURE
  • 1991-02. Effects of tomato cultivar, leaf age, and bacterial strain on transformation by Agrobacterium tumefaciens in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1994-09. The small, versatilepPZP family ofAgrobacterium binary vectors for plant transformation in PLANT MOLECULAR BIOLOGY
  • 1994. Genetic manipulation of Agrobacterium tumefaciens strains to improve transformation of recalcitrant plant species in PLANT MOLECULAR BIOLOGY MANUAL
  • 1989-05. Transformation of white spruce and other conifer species byAgrobacterium tumefaciens in PLANT CELL REPORTS
  • 1994. Agrobacterium-mediated gene transfer to plant cells: cointegrate and binary vector systems in PLANT MOLECULAR BIOLOGY MANUAL
  • 1993. Transformation in Spruce (Picea Species) in PLANT PROTOPLASTS AND GENETIC ENGINEERING IV
  • 1996-10. Somatic embryogenesis in conifers—A case study of induction and development of somatic embryos in Picea abies in PLANT GROWTH REGULATION
  • 1987-12. Assaying chimeric genes in plants: The GUS gene fusion system in PLANT MOLECULAR BIOLOGY REPORTER
  • 1993-07. NewAgrobacterium helper plasmids for gene transfer to plants in TRANSGENIC RESEARCH
  • 1986-11. Plantlet regeneration via somatic embryogenesis from subcultured callus of mature embryos ofPicea abies (Norway spruce) in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY
  • 1996-06. Genetic transformation of cassava (Manihot esculenta Crantz) in NATURE BIOTECHNOLOGY
  • 1986-09. The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vector in MOLECULAR GENETICS AND GENOMICS
  • 1989-01. Norway spruce somatic embryogenesis: high-frequency initiation from light-cultured mature embryos in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1996-06. High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens in NATURE BIOTECHNOLOGY
  • 1986-07. Gene Transfer into Loblolly Pine by Agrobacterium tumefaciens in NATURE BIOTECHNOLOGY
  • 1992-12. Multiple copies of virG enhance the transient transformation of celery, carrot and rice tissues by Agrobacterium tumefaciens in PLANT MOLECULAR BIOLOGY
  • 1993. DNA Transfer in Conifers in CLONAL FORESTRY I
  • 1990-10. Transformation of cultured cells of Chenopodium quinoa by binary vectors that carry a fragment of DNA from the virulence region of pTiBo542 in PLANT CELL REPORTS
  • 1988-05. Analysis of the T-DNA structure in a large number of transgenic petunias generated by Agrobacterium-mediated transformation in PLANT MOLECULAR BIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1023/a:1006126609534

    DOI

    http://dx.doi.org/10.1023/a:1006126609534

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1022561351

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/10092170


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Acetophenones", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Agrobacterium tumefaciens", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Base Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Culture Techniques", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA, Bacterial", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Dose-Response Relationship, Drug", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Molecular Sequence Data", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Pinus taeda", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Transformation, Genetic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Virulence", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "North Carolina State University", 
              "id": "https://www.grid.ac/institutes/grid.40803.3f", 
              "name": [
                "Forest Biotechnology Group, North Carolina State University, 27695, Raleigh, NC, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Richard Wenck", 
            "givenName": "Allan", 
            "id": "sg:person.01315441060.31", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01315441060.31"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "North Carolina State University", 
              "id": "https://www.grid.ac/institutes/grid.40803.3f", 
              "name": [
                "Forest Biotechnology Group, North Carolina State University, 27695, Raleigh, NC, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Quinn", 
            "givenName": "Michelle", 
            "id": "sg:person.01266072576.80", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01266072576.80"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "North Carolina State University", 
              "id": "https://www.grid.ac/institutes/grid.40803.3f", 
              "name": [
                "Forest Biotechnology Group, North Carolina State University, 27695, Raleigh, NC, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Whetten", 
            "givenName": "Ross W.", 
            "id": "sg:person.0715342240.05", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0715342240.05"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Georgia Institute of Technology", 
              "id": "https://www.grid.ac/institutes/grid.213917.f", 
              "name": [
                "Institute of Paper Science and Technology, 30318, Atlanta, GA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Pullman", 
            "givenName": "Gerald", 
            "id": "sg:person.01354707361.82", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01354707361.82"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "North Carolina State University", 
              "id": "https://www.grid.ac/institutes/grid.40803.3f", 
              "name": [
                "Forest Biotechnology Group, North Carolina State University, 27695, Raleigh, NC, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Sederoff", 
            "givenName": "Ronald", 
            "id": "sg:person.01356507525.20", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01356507525.20"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1007/bf00735769", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000228520", 
              "https://doi.org/10.1007/bf00735769"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf01977351", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000235724", 
              "https://doi.org/10.1007/bf01977351"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf01977351", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000235724", 
              "https://doi.org/10.1007/bf01977351"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02623484", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000709413", 
              "https://doi.org/10.1007/bf02623484"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02623484", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000709413", 
              "https://doi.org/10.1007/bf02623484"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1080/07352689709701951", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002441050"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00039739", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004191435", 
              "https://doi.org/10.1007/bf00039739"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00039739", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004191435", 
              "https://doi.org/10.1007/bf00039739"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/jxb/48.1.151", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007157087"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00014672", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007506198", 
              "https://doi.org/10.1007/bf00014672"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00014672", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007506198", 
              "https://doi.org/10.1007/bf00014672"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1104/pp.97.2.832", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010133467"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00192812", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011448130", 
              "https://doi.org/10.1007/bf00192812"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0003-2697(83)90418-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012973560"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt0696-736", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013865498", 
              "https://doi.org/10.1038/nbt0696-736"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00331014", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013909718", 
              "https://doi.org/10.1007/bf00331014"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1104/pp.92.4.1226", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014023218"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt0786-647", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017642049", 
              "https://doi.org/10.1038/nbt0786-647"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02667740", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017993505", 
              "https://doi.org/10.1007/bf02667740"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02667740", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017993505", 
              "https://doi.org/10.1007/bf02667740"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/303179a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018421874", 
              "https://doi.org/10.1038/303179a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-3-642-84175-0_15", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019363517", 
              "https://doi.org/10.1007/978-3-642-84175-0_15"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02632217", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019970975", 
              "https://doi.org/10.1007/bf02632217"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1046/j.1365-313x.1994.6020271.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023112743"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.91.16.7603", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024084209"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00232856", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024486599", 
              "https://doi.org/10.1007/bf00232856"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00232856", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024486599", 
              "https://doi.org/10.1007/bf00232856"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0294-3506(99)80228-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025351510"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0294-3506(99)80228-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025351510"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-3-642-78037-0_25", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025418388", 
              "https://doi.org/10.1007/978-3-642-78037-0_25"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00027393", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026960131", 
              "https://doi.org/10.1007/bf00027393"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00027393", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026960131", 
              "https://doi.org/10.1007/bf00027393"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1017/cbo9780511752261.013", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027177143"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00036518", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028684075", 
              "https://doi.org/10.1007/bf00036518"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00036518", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028684075", 
              "https://doi.org/10.1007/bf00036518"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf01007700", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030833076", 
              "https://doi.org/10.1007/bf01007700"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/23.6.1087", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032701642"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0022-2836(75)80083-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033296365"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1139/b91-237", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033875506"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-3-662-03091-2_29", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035060525", 
              "https://doi.org/10.1007/978-3-662-03091-2_29"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00024050", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037667746", 
              "https://doi.org/10.1007/bf00024050"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00024050", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037667746", 
              "https://doi.org/10.1007/bf00024050"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00040525", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037679416", 
              "https://doi.org/10.1007/bf00040525"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00040525", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037679416", 
              "https://doi.org/10.1007/bf00040525"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-94-011-0511-8_3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039606618", 
              "https://doi.org/10.1007/978-94-011-0511-8_3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1046/j.1365-313x.1995.7040661.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045311524"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00028894", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045435266", 
              "https://doi.org/10.1007/bf00028894"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00028894", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045435266", 
              "https://doi.org/10.1007/bf00028894"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0176-1617(11)82125-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046487175"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-94-011-0511-8_6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047109668", 
              "https://doi.org/10.1007/978-94-011-0511-8_6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt0696-745", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048394884", 
              "https://doi.org/10.1038/nbt0696-745"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1104/pp.79.2.568", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052662880"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/jxb/45.10.1441", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1059857929"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1104/pp.115.3.971", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060841550"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.273.5278.1107", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062553949"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jb.169.10.4417-4425.1987", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062715466"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1017/cbo9780511752261.005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1089385160"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-94-017-5294-7_3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1089711437", 
              "https://doi.org/10.1007/978-94-017-5294-7_3"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1999-02", 
        "datePublishedReg": "1999-02-01", 
        "description": "Agrobacterium-mediated gene transfer is the method of choice for many plant biotechnology laboratories; however, large-scale use of this organism in conifer transformation has been limited by difficult propagation of explant material, selection efficiencies and low transformation frequency. We have analyzed co-cultivation conditions and different disarmed strains of Agrobacterium to improve transformation. Additional copies of virulence genes were added to three common disarmed strains. These extra virulence genes included either a constitutively active virG or extra copies of virG and virB, both from pTiBo542. In experiments with Norway spruce, we increased transformation efficiencies 1000-fold from initial experiments where little or no transient expression was detected. Over 100 transformed lines expressing the marker gene beta-glucuronidase (GUS) were generated from rapidly dividing embryogenic suspension-cultured cells co-cultivated with Agrobacterium. GUS activity was used to monitor transient expression and to further test lines selected on kanamycin-containing medium. In loblolly pine, transient expression increased 10-fold utilizing modified Agrobacterium strains. Agrobacterium-mediated gene transfer is a useful technique for large-scale generation of transgenic Norway spruce and may prove useful for other conifer species.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1023/a:1006126609534", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1101246", 
            "issn": [
              "0167-4412", 
              "1573-5028"
            ], 
            "name": "Plant Molecular Biology", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "3", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "39"
          }
        ], 
        "name": "High-efficiency Agrobacterium-mediated transformation of Norway spruce (Picea abies) and loblolly pine (Pinus taeda)", 
        "pagination": "407-416", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "6b7ccddc69f9f6401143e2aca054e7267b67491f4224730da81318344680c3e4"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "10092170"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9106343"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1023/a:1006126609534"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1022561351"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1023/a:1006126609534", 
          "https://app.dimensions.ai/details/publication/pub.1022561351"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T21:34", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8687_00000499.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1023/A:1006126609534"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1023/a:1006126609534'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1023/a:1006126609534'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1023/a:1006126609534'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1023/a:1006126609534'


     

    This table displays all metadata directly associated to this object as RDF triples.

    304 TRIPLES      21 PREDICATES      85 URIs      31 LITERALS      19 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1023/a:1006126609534 schema:about N001724e673954643a07739ba66ab06b1
    2 N1edb850b655f4ad492affcca133b1c61
    3 N4b11dfff7ad9456fb5c848ce9df71304
    4 N5e87acc1784942d8874f14d3a33f33d8
    5 N6c87852ea4bc43f1910a710adf26608b
    6 N76c8fd525a6a4427a66828d2dee7ec4d
    7 N9cdca9b5a8814403a11edf952769b13a
    8 Na5d47099b5444b53923493c4a9adfaf5
    9 Nc919bfe450554a1395766cac133809f3
    10 Nffded60728db451f819d750633f0e933
    11 anzsrc-for:06
    12 anzsrc-for:0604
    13 schema:author Ncedc817c63b643e7870940e02bda1191
    14 schema:citation sg:pub.10.1007/978-3-642-78037-0_25
    15 sg:pub.10.1007/978-3-642-84175-0_15
    16 sg:pub.10.1007/978-3-662-03091-2_29
    17 sg:pub.10.1007/978-94-011-0511-8_3
    18 sg:pub.10.1007/978-94-011-0511-8_6
    19 sg:pub.10.1007/978-94-017-5294-7_3
    20 sg:pub.10.1007/bf00014672
    21 sg:pub.10.1007/bf00024050
    22 sg:pub.10.1007/bf00027393
    23 sg:pub.10.1007/bf00028894
    24 sg:pub.10.1007/bf00036518
    25 sg:pub.10.1007/bf00039739
    26 sg:pub.10.1007/bf00040525
    27 sg:pub.10.1007/bf00192812
    28 sg:pub.10.1007/bf00232856
    29 sg:pub.10.1007/bf00331014
    30 sg:pub.10.1007/bf00735769
    31 sg:pub.10.1007/bf01007700
    32 sg:pub.10.1007/bf01977351
    33 sg:pub.10.1007/bf02623484
    34 sg:pub.10.1007/bf02632217
    35 sg:pub.10.1007/bf02667740
    36 sg:pub.10.1038/303179a0
    37 sg:pub.10.1038/nbt0696-736
    38 sg:pub.10.1038/nbt0696-745
    39 sg:pub.10.1038/nbt0786-647
    40 https://doi.org/10.1016/0003-2697(83)90418-9
    41 https://doi.org/10.1016/s0022-2836(75)80083-0
    42 https://doi.org/10.1016/s0176-1617(11)82125-5
    43 https://doi.org/10.1016/s0294-3506(99)80228-2
    44 https://doi.org/10.1017/cbo9780511752261.005
    45 https://doi.org/10.1017/cbo9780511752261.013
    46 https://doi.org/10.1046/j.1365-313x.1994.6020271.x
    47 https://doi.org/10.1046/j.1365-313x.1995.7040661.x
    48 https://doi.org/10.1073/pnas.91.16.7603
    49 https://doi.org/10.1080/07352689709701951
    50 https://doi.org/10.1093/jxb/45.10.1441
    51 https://doi.org/10.1093/jxb/48.1.151
    52 https://doi.org/10.1093/nar/23.6.1087
    53 https://doi.org/10.1104/pp.115.3.971
    54 https://doi.org/10.1104/pp.79.2.568
    55 https://doi.org/10.1104/pp.92.4.1226
    56 https://doi.org/10.1104/pp.97.2.832
    57 https://doi.org/10.1126/science.273.5278.1107
    58 https://doi.org/10.1128/jb.169.10.4417-4425.1987
    59 https://doi.org/10.1139/b91-237
    60 schema:datePublished 1999-02
    61 schema:datePublishedReg 1999-02-01
    62 schema:description Agrobacterium-mediated gene transfer is the method of choice for many plant biotechnology laboratories; however, large-scale use of this organism in conifer transformation has been limited by difficult propagation of explant material, selection efficiencies and low transformation frequency. We have analyzed co-cultivation conditions and different disarmed strains of Agrobacterium to improve transformation. Additional copies of virulence genes were added to three common disarmed strains. These extra virulence genes included either a constitutively active virG or extra copies of virG and virB, both from pTiBo542. In experiments with Norway spruce, we increased transformation efficiencies 1000-fold from initial experiments where little or no transient expression was detected. Over 100 transformed lines expressing the marker gene beta-glucuronidase (GUS) were generated from rapidly dividing embryogenic suspension-cultured cells co-cultivated with Agrobacterium. GUS activity was used to monitor transient expression and to further test lines selected on kanamycin-containing medium. In loblolly pine, transient expression increased 10-fold utilizing modified Agrobacterium strains. Agrobacterium-mediated gene transfer is a useful technique for large-scale generation of transgenic Norway spruce and may prove useful for other conifer species.
    63 schema:genre research_article
    64 schema:inLanguage en
    65 schema:isAccessibleForFree false
    66 schema:isPartOf N479acca1b5114c519c566439f5f061fe
    67 N4d4feb7fb0f645b38038d8e122605f38
    68 sg:journal.1101246
    69 schema:name High-efficiency Agrobacterium-mediated transformation of Norway spruce (Picea abies) and loblolly pine (Pinus taeda)
    70 schema:pagination 407-416
    71 schema:productId N6eb6bc4f6d804c928af68154c7ec6c72
    72 N930c559375aa43d4853819dbbb3ccf0a
    73 Naa992ebc23854d759a26f730ad491f8b
    74 Nb92d55a3b6754c54ae292db9d53f35a0
    75 Nee9808a05fe44ff58ffbe057aa34aaa1
    76 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022561351
    77 https://doi.org/10.1023/a:1006126609534
    78 schema:sdDatePublished 2019-04-10T21:34
    79 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    80 schema:sdPublisher N001a497914b842b7bde094bfb4aac0e1
    81 schema:url http://link.springer.com/10.1023/A:1006126609534
    82 sgo:license sg:explorer/license/
    83 sgo:sdDataset articles
    84 rdf:type schema:ScholarlyArticle
    85 N001724e673954643a07739ba66ab06b1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    86 schema:name Molecular Sequence Data
    87 rdf:type schema:DefinedTerm
    88 N001a497914b842b7bde094bfb4aac0e1 schema:name Springer Nature - SN SciGraph project
    89 rdf:type schema:Organization
    90 N1edb850b655f4ad492affcca133b1c61 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    91 schema:name Dose-Response Relationship, Drug
    92 rdf:type schema:DefinedTerm
    93 N479acca1b5114c519c566439f5f061fe schema:volumeNumber 39
    94 rdf:type schema:PublicationVolume
    95 N482ac9af87c040b1a6f17eff192e9761 rdf:first sg:person.01354707361.82
    96 rdf:rest N68f952dd26234c3e8571794aaf5b3250
    97 N4b11dfff7ad9456fb5c848ce9df71304 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    98 schema:name Acetophenones
    99 rdf:type schema:DefinedTerm
    100 N4d4feb7fb0f645b38038d8e122605f38 schema:issueNumber 3
    101 rdf:type schema:PublicationIssue
    102 N5e87acc1784942d8874f14d3a33f33d8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    103 schema:name Base Sequence
    104 rdf:type schema:DefinedTerm
    105 N68a1eedad1404af9b1edb2df3d0a7b93 rdf:first sg:person.0715342240.05
    106 rdf:rest N482ac9af87c040b1a6f17eff192e9761
    107 N68f952dd26234c3e8571794aaf5b3250 rdf:first sg:person.01356507525.20
    108 rdf:rest rdf:nil
    109 N6c87852ea4bc43f1910a710adf26608b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    110 schema:name DNA, Bacterial
    111 rdf:type schema:DefinedTerm
    112 N6eb6bc4f6d804c928af68154c7ec6c72 schema:name doi
    113 schema:value 10.1023/a:1006126609534
    114 rdf:type schema:PropertyValue
    115 N76c8fd525a6a4427a66828d2dee7ec4d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    116 schema:name Agrobacterium tumefaciens
    117 rdf:type schema:DefinedTerm
    118 N930c559375aa43d4853819dbbb3ccf0a schema:name dimensions_id
    119 schema:value pub.1022561351
    120 rdf:type schema:PropertyValue
    121 N9cdca9b5a8814403a11edf952769b13a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    122 schema:name Pinus taeda
    123 rdf:type schema:DefinedTerm
    124 Na5d47099b5444b53923493c4a9adfaf5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    125 schema:name Transformation, Genetic
    126 rdf:type schema:DefinedTerm
    127 Naa992ebc23854d759a26f730ad491f8b schema:name readcube_id
    128 schema:value 6b7ccddc69f9f6401143e2aca054e7267b67491f4224730da81318344680c3e4
    129 rdf:type schema:PropertyValue
    130 Nb92d55a3b6754c54ae292db9d53f35a0 schema:name nlm_unique_id
    131 schema:value 9106343
    132 rdf:type schema:PropertyValue
    133 Nc919bfe450554a1395766cac133809f3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    134 schema:name Culture Techniques
    135 rdf:type schema:DefinedTerm
    136 Ncedc817c63b643e7870940e02bda1191 rdf:first sg:person.01315441060.31
    137 rdf:rest Ncfb704977f934979bc65a338e29ed55e
    138 Ncfb704977f934979bc65a338e29ed55e rdf:first sg:person.01266072576.80
    139 rdf:rest N68a1eedad1404af9b1edb2df3d0a7b93
    140 Nee9808a05fe44ff58ffbe057aa34aaa1 schema:name pubmed_id
    141 schema:value 10092170
    142 rdf:type schema:PropertyValue
    143 Nffded60728db451f819d750633f0e933 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    144 schema:name Virulence
    145 rdf:type schema:DefinedTerm
    146 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    147 schema:name Biological Sciences
    148 rdf:type schema:DefinedTerm
    149 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    150 schema:name Genetics
    151 rdf:type schema:DefinedTerm
    152 sg:journal.1101246 schema:issn 0167-4412
    153 1573-5028
    154 schema:name Plant Molecular Biology
    155 rdf:type schema:Periodical
    156 sg:person.01266072576.80 schema:affiliation https://www.grid.ac/institutes/grid.40803.3f
    157 schema:familyName Quinn
    158 schema:givenName Michelle
    159 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01266072576.80
    160 rdf:type schema:Person
    161 sg:person.01315441060.31 schema:affiliation https://www.grid.ac/institutes/grid.40803.3f
    162 schema:familyName Richard Wenck
    163 schema:givenName Allan
    164 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01315441060.31
    165 rdf:type schema:Person
    166 sg:person.01354707361.82 schema:affiliation https://www.grid.ac/institutes/grid.213917.f
    167 schema:familyName Pullman
    168 schema:givenName Gerald
    169 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01354707361.82
    170 rdf:type schema:Person
    171 sg:person.01356507525.20 schema:affiliation https://www.grid.ac/institutes/grid.40803.3f
    172 schema:familyName Sederoff
    173 schema:givenName Ronald
    174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01356507525.20
    175 rdf:type schema:Person
    176 sg:person.0715342240.05 schema:affiliation https://www.grid.ac/institutes/grid.40803.3f
    177 schema:familyName Whetten
    178 schema:givenName Ross W.
    179 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0715342240.05
    180 rdf:type schema:Person
    181 sg:pub.10.1007/978-3-642-78037-0_25 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025418388
    182 https://doi.org/10.1007/978-3-642-78037-0_25
    183 rdf:type schema:CreativeWork
    184 sg:pub.10.1007/978-3-642-84175-0_15 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019363517
    185 https://doi.org/10.1007/978-3-642-84175-0_15
    186 rdf:type schema:CreativeWork
    187 sg:pub.10.1007/978-3-662-03091-2_29 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035060525
    188 https://doi.org/10.1007/978-3-662-03091-2_29
    189 rdf:type schema:CreativeWork
    190 sg:pub.10.1007/978-94-011-0511-8_3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039606618
    191 https://doi.org/10.1007/978-94-011-0511-8_3
    192 rdf:type schema:CreativeWork
    193 sg:pub.10.1007/978-94-011-0511-8_6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047109668
    194 https://doi.org/10.1007/978-94-011-0511-8_6
    195 rdf:type schema:CreativeWork
    196 sg:pub.10.1007/978-94-017-5294-7_3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1089711437
    197 https://doi.org/10.1007/978-94-017-5294-7_3
    198 rdf:type schema:CreativeWork
    199 sg:pub.10.1007/bf00014672 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007506198
    200 https://doi.org/10.1007/bf00014672
    201 rdf:type schema:CreativeWork
    202 sg:pub.10.1007/bf00024050 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037667746
    203 https://doi.org/10.1007/bf00024050
    204 rdf:type schema:CreativeWork
    205 sg:pub.10.1007/bf00027393 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026960131
    206 https://doi.org/10.1007/bf00027393
    207 rdf:type schema:CreativeWork
    208 sg:pub.10.1007/bf00028894 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045435266
    209 https://doi.org/10.1007/bf00028894
    210 rdf:type schema:CreativeWork
    211 sg:pub.10.1007/bf00036518 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028684075
    212 https://doi.org/10.1007/bf00036518
    213 rdf:type schema:CreativeWork
    214 sg:pub.10.1007/bf00039739 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004191435
    215 https://doi.org/10.1007/bf00039739
    216 rdf:type schema:CreativeWork
    217 sg:pub.10.1007/bf00040525 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037679416
    218 https://doi.org/10.1007/bf00040525
    219 rdf:type schema:CreativeWork
    220 sg:pub.10.1007/bf00192812 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011448130
    221 https://doi.org/10.1007/bf00192812
    222 rdf:type schema:CreativeWork
    223 sg:pub.10.1007/bf00232856 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024486599
    224 https://doi.org/10.1007/bf00232856
    225 rdf:type schema:CreativeWork
    226 sg:pub.10.1007/bf00331014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013909718
    227 https://doi.org/10.1007/bf00331014
    228 rdf:type schema:CreativeWork
    229 sg:pub.10.1007/bf00735769 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000228520
    230 https://doi.org/10.1007/bf00735769
    231 rdf:type schema:CreativeWork
    232 sg:pub.10.1007/bf01007700 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030833076
    233 https://doi.org/10.1007/bf01007700
    234 rdf:type schema:CreativeWork
    235 sg:pub.10.1007/bf01977351 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000235724
    236 https://doi.org/10.1007/bf01977351
    237 rdf:type schema:CreativeWork
    238 sg:pub.10.1007/bf02623484 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000709413
    239 https://doi.org/10.1007/bf02623484
    240 rdf:type schema:CreativeWork
    241 sg:pub.10.1007/bf02632217 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019970975
    242 https://doi.org/10.1007/bf02632217
    243 rdf:type schema:CreativeWork
    244 sg:pub.10.1007/bf02667740 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017993505
    245 https://doi.org/10.1007/bf02667740
    246 rdf:type schema:CreativeWork
    247 sg:pub.10.1038/303179a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018421874
    248 https://doi.org/10.1038/303179a0
    249 rdf:type schema:CreativeWork
    250 sg:pub.10.1038/nbt0696-736 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013865498
    251 https://doi.org/10.1038/nbt0696-736
    252 rdf:type schema:CreativeWork
    253 sg:pub.10.1038/nbt0696-745 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048394884
    254 https://doi.org/10.1038/nbt0696-745
    255 rdf:type schema:CreativeWork
    256 sg:pub.10.1038/nbt0786-647 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017642049
    257 https://doi.org/10.1038/nbt0786-647
    258 rdf:type schema:CreativeWork
    259 https://doi.org/10.1016/0003-2697(83)90418-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012973560
    260 rdf:type schema:CreativeWork
    261 https://doi.org/10.1016/s0022-2836(75)80083-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033296365
    262 rdf:type schema:CreativeWork
    263 https://doi.org/10.1016/s0176-1617(11)82125-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046487175
    264 rdf:type schema:CreativeWork
    265 https://doi.org/10.1016/s0294-3506(99)80228-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025351510
    266 rdf:type schema:CreativeWork
    267 https://doi.org/10.1017/cbo9780511752261.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1089385160
    268 rdf:type schema:CreativeWork
    269 https://doi.org/10.1017/cbo9780511752261.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027177143
    270 rdf:type schema:CreativeWork
    271 https://doi.org/10.1046/j.1365-313x.1994.6020271.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1023112743
    272 rdf:type schema:CreativeWork
    273 https://doi.org/10.1046/j.1365-313x.1995.7040661.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1045311524
    274 rdf:type schema:CreativeWork
    275 https://doi.org/10.1073/pnas.91.16.7603 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024084209
    276 rdf:type schema:CreativeWork
    277 https://doi.org/10.1080/07352689709701951 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002441050
    278 rdf:type schema:CreativeWork
    279 https://doi.org/10.1093/jxb/45.10.1441 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059857929
    280 rdf:type schema:CreativeWork
    281 https://doi.org/10.1093/jxb/48.1.151 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007157087
    282 rdf:type schema:CreativeWork
    283 https://doi.org/10.1093/nar/23.6.1087 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032701642
    284 rdf:type schema:CreativeWork
    285 https://doi.org/10.1104/pp.115.3.971 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060841550
    286 rdf:type schema:CreativeWork
    287 https://doi.org/10.1104/pp.79.2.568 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052662880
    288 rdf:type schema:CreativeWork
    289 https://doi.org/10.1104/pp.92.4.1226 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014023218
    290 rdf:type schema:CreativeWork
    291 https://doi.org/10.1104/pp.97.2.832 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010133467
    292 rdf:type schema:CreativeWork
    293 https://doi.org/10.1126/science.273.5278.1107 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062553949
    294 rdf:type schema:CreativeWork
    295 https://doi.org/10.1128/jb.169.10.4417-4425.1987 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062715466
    296 rdf:type schema:CreativeWork
    297 https://doi.org/10.1139/b91-237 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033875506
    298 rdf:type schema:CreativeWork
    299 https://www.grid.ac/institutes/grid.213917.f schema:alternateName Georgia Institute of Technology
    300 schema:name Institute of Paper Science and Technology, 30318, Atlanta, GA, USA
    301 rdf:type schema:Organization
    302 https://www.grid.ac/institutes/grid.40803.3f schema:alternateName North Carolina State University
    303 schema:name Forest Biotechnology Group, North Carolina State University, 27695, Raleigh, NC, USA
    304 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...