Time-resolved electron transfer at the donor side of Rhodopseudomonas viridis photosynthetic reaction centers in whole cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-03

AUTHORS

Fabrice Rappaport, Daniel Béal, André Verméglio, Pierre Joliot

ABSTRACT

We have studied the electron transfer reactions from the tetraheme cytochrome of Rhodopseudomonas viridis to the oxidized primary donor in whole cells with a new high sensitivity spectrophotometer. In this apparatus the monochromatic detecting flashes are provided by a YAG pumped Optical Parametric Oscillator, allowing a 10 ns time resolution. When four hemes are reduced the observed electron transfer reaction sequence is the following: first the low-potential c552 heme (the number refers to the maximum absorption wavelength in the alpha-band region) is oxidized with a half time of 130 ns, in agreement with previous reports of measurements performed with purified reaction centers. Then, the electron hole is transferred to the low potential c554 heme with a half time of 2.6 µs. When only the two high potential hemes are reduced the observed electron transfer sequence is the following: oxidation of the high potential c559 heme in the hundreds of ns time range (410 ns), reduction of this heme by the high potential c556 heme in the µs time range (2.7 µs). This confirms the first steps of electron transfer observed in isolated reaction centers. However, in the microsecond time domain, the overall amount of oxidized hemes increases suggesting that, in vivo, the equilibrium constant between the P+/P and the c559ox/c559red couples is significantly lower than expected from the difference in their midpoint potentials. More... »

PAGES

317-323

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1005930018775

DOI

http://dx.doi.org/10.1023/a:1005930018775

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1021972178


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0607", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Plant Biology", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Institut de Biologie Physico-Chimique, CNRS UPR, 9072, 13 rue Pierre et Marie Curie, 75005, PARIS, France", 
          "id": "http://www.grid.ac/institutes/grid.450875.b", 
          "name": [
            "Institut de Biologie Physico-Chimique, CNRS UPR, 9072, 13 rue Pierre et Marie Curie, 75005, PARIS, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rappaport", 
        "givenName": "Fabrice", 
        "id": "sg:person.01103560265.56", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01103560265.56"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institut de Biologie Physico-Chimique, CNRS UPR, 9072, 13 rue Pierre et Marie Curie, 75005, PARIS, France", 
          "id": "http://www.grid.ac/institutes/grid.450875.b", 
          "name": [
            "Institut de Biologie Physico-Chimique, CNRS UPR, 9072, 13 rue Pierre et Marie Curie, 75005, PARIS, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "B\u00e9al", 
        "givenName": "Daniel", 
        "id": "sg:person.0636622234.64", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0636622234.64"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "CEA Cadarache, DEVM/Laboratoire de Bio\u00e9nerg\u00e9tique Cellulaire, 13108, St Paul-lez-Durance, France", 
          "id": "http://www.grid.ac/institutes/grid.457335.3", 
          "name": [
            "CEA Cadarache, DEVM/Laboratoire de Bio\u00e9nerg\u00e9tique Cellulaire, 13108, St Paul-lez-Durance, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Verm\u00e9glio", 
        "givenName": "Andr\u00e9", 
        "id": "sg:person.01207002310.01", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01207002310.01"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institut de Biologie Physico-Chimique, CNRS UPR, 9072, 13 rue Pierre et Marie Curie, 75005, PARIS, France", 
          "id": "http://www.grid.ac/institutes/grid.450875.b", 
          "name": [
            "Institut de Biologie Physico-Chimique, CNRS UPR, 9072, 13 rue Pierre et Marie Curie, 75005, PARIS, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Joliot", 
        "givenName": "Pierre", 
        "id": "sg:person.01324041054.45", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01324041054.45"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/318618a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020599081", 
          "https://doi.org/10.1038/318618a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00047946", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045641142", 
          "https://doi.org/10.1007/bf00047946"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/355796a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032183790", 
          "https://doi.org/10.1038/355796a0"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1998-03", 
    "datePublishedReg": "1998-03-01", 
    "description": "We have studied the electron transfer reactions from the tetraheme cytochrome of Rhodopseudomonas viridis to the oxidized primary donor in whole cells with a new high sensitivity spectrophotometer. In this apparatus the monochromatic detecting flashes are provided by a YAG pumped Optical Parametric Oscillator, allowing a 10 ns time resolution. When four hemes are reduced the observed electron transfer reaction sequence is the following: first the low-potential c552 heme (the number refers to the maximum absorption wavelength in the alpha-band region) is oxidized with a half time of 130 ns, in agreement with previous reports of measurements performed with purified reaction centers. Then, the electron hole is transferred to the low potential c554 heme with a half time of 2.6 \u00b5s. When only the two high potential hemes are reduced the observed electron transfer sequence is the following: oxidation of the high potential c559 heme in the hundreds of ns time range (410 ns), reduction of this heme by the high potential c556 heme in the \u00b5s time range (2.7 \u00b5s). This confirms the first steps of electron transfer observed in isolated reaction centers. However, in the microsecond time domain, the overall amount of oxidized hemes increases suggesting that, in vivo, the equilibrium constant between the P+/P and the c559ox/c559red couples is significantly lower than expected from the difference in their midpoint potentials.", 
    "genre": "article", 
    "id": "sg:pub.10.1023/a:1005930018775", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1022986", 
        "issn": [
          "0166-8595", 
          "1573-5079"
        ], 
        "name": "Photosynthesis Research", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "2-3", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "55"
      }
    ], 
    "keywords": [
      "reaction centers", 
      "time-resolved electron transfer", 
      "optical parametric oscillator", 
      "ns time resolution", 
      "ns time range", 
      "photosynthetic reaction centers", 
      "Rhodopseudomonas viridis photosynthetic reaction centers", 
      "new high sensitivity", 
      "microsecond time domain", 
      "parametric oscillator", 
      "electron transfer sequence", 
      "electron transfer", 
      "electron holes", 
      "time resolution", 
      "electron transfer reactions", 
      "time range", 
      "Rhodopseudomonas viridis", 
      "transfer reactions", 
      "donor side", 
      "primary donor", 
      "heme increases", 
      "highest potential heme", 
      "high sensitivity", 
      "ns", 
      "YAG", 
      "time domain", 
      "holes", 
      "transfer sequence", 
      "tetraheme cytochrome", 
      "oscillator", 
      "equilibrium constants", 
      "transfer", 
      "resolution", 
      "midpoint potential", 
      "measurements", 
      "flashes", 
      "range", 
      "constants", 
      "agreement", 
      "center", 
      "apparatus", 
      "hundreds", 
      "potential heme", 
      "reaction", 
      "whole cells", 
      "sensitivity", 
      "time", 
      "potential", 
      "first step", 
      "heme", 
      "reaction sequence", 
      "viridis", 
      "half times", 
      "couples", 
      "side", 
      "donors", 
      "step", 
      "oxidation", 
      "increase", 
      "cytochrome", 
      "domain", 
      "amount", 
      "previous reports", 
      "reduction", 
      "sequence", 
      "differences", 
      "cells", 
      "overall amount", 
      "vivo", 
      "report", 
      "observed electron transfer reaction sequence", 
      "electron transfer reaction sequence", 
      "transfer reaction sequence", 
      "low-potential c552 heme", 
      "c552 heme", 
      "low potential c554 heme", 
      "potential c554 heme", 
      "c554 heme", 
      "observed electron transfer sequence", 
      "high potential c559 heme", 
      "potential c559 heme", 
      "c559 heme", 
      "high potential c556 heme", 
      "potential c556 heme", 
      "c556 heme", 
      "c559ox/", 
      "viridis photosynthetic reaction centers"
    ], 
    "name": "Time-resolved electron transfer at the donor side of Rhodopseudomonas viridis photosynthetic reaction centers in whole cells", 
    "pagination": "317-323", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1021972178"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1023/a:1005930018775"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1023/a:1005930018775", 
      "https://app.dimensions.ai/details/publication/pub.1021972178"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-01-01T18:07", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_277.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1023/a:1005930018775"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1023/a:1005930018775'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1023/a:1005930018775'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1023/a:1005930018775'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1023/a:1005930018775'


 

This table displays all metadata directly associated to this object as RDF triples.

189 TRIPLES      22 PREDICATES      118 URIs      105 LITERALS      6 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1023/a:1005930018775 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 anzsrc-for:0604
4 anzsrc-for:0607
5 schema:author Nd13a9e93cdf74847a7ddcbd2b8a0556c
6 schema:citation sg:pub.10.1007/bf00047946
7 sg:pub.10.1038/318618a0
8 sg:pub.10.1038/355796a0
9 schema:datePublished 1998-03
10 schema:datePublishedReg 1998-03-01
11 schema:description We have studied the electron transfer reactions from the tetraheme cytochrome of Rhodopseudomonas viridis to the oxidized primary donor in whole cells with a new high sensitivity spectrophotometer. In this apparatus the monochromatic detecting flashes are provided by a YAG pumped Optical Parametric Oscillator, allowing a 10 ns time resolution. When four hemes are reduced the observed electron transfer reaction sequence is the following: first the low-potential c552 heme (the number refers to the maximum absorption wavelength in the alpha-band region) is oxidized with a half time of 130 ns, in agreement with previous reports of measurements performed with purified reaction centers. Then, the electron hole is transferred to the low potential c554 heme with a half time of 2.6 µs. When only the two high potential hemes are reduced the observed electron transfer sequence is the following: oxidation of the high potential c559 heme in the hundreds of ns time range (410 ns), reduction of this heme by the high potential c556 heme in the µs time range (2.7 µs). This confirms the first steps of electron transfer observed in isolated reaction centers. However, in the microsecond time domain, the overall amount of oxidized hemes increases suggesting that, in vivo, the equilibrium constant between the P+/P and the c559ox/c559red couples is significantly lower than expected from the difference in their midpoint potentials.
12 schema:genre article
13 schema:inLanguage en
14 schema:isAccessibleForFree false
15 schema:isPartOf N05c7731510c54ad79a35c04fa14d5154
16 N6e4313609b5e419fa5e48249840de978
17 sg:journal.1022986
18 schema:keywords Rhodopseudomonas viridis
19 Rhodopseudomonas viridis photosynthetic reaction centers
20 YAG
21 agreement
22 amount
23 apparatus
24 c552 heme
25 c554 heme
26 c556 heme
27 c559 heme
28 c559ox/
29 cells
30 center
31 constants
32 couples
33 cytochrome
34 differences
35 domain
36 donor side
37 donors
38 electron holes
39 electron transfer
40 electron transfer reaction sequence
41 electron transfer reactions
42 electron transfer sequence
43 equilibrium constants
44 first step
45 flashes
46 half times
47 heme
48 heme increases
49 high potential c556 heme
50 high potential c559 heme
51 high sensitivity
52 highest potential heme
53 holes
54 hundreds
55 increase
56 low potential c554 heme
57 low-potential c552 heme
58 measurements
59 microsecond time domain
60 midpoint potential
61 new high sensitivity
62 ns
63 ns time range
64 ns time resolution
65 observed electron transfer reaction sequence
66 observed electron transfer sequence
67 optical parametric oscillator
68 oscillator
69 overall amount
70 oxidation
71 parametric oscillator
72 photosynthetic reaction centers
73 potential
74 potential c554 heme
75 potential c556 heme
76 potential c559 heme
77 potential heme
78 previous reports
79 primary donor
80 range
81 reaction
82 reaction centers
83 reaction sequence
84 reduction
85 report
86 resolution
87 sensitivity
88 sequence
89 side
90 step
91 tetraheme cytochrome
92 time
93 time domain
94 time range
95 time resolution
96 time-resolved electron transfer
97 transfer
98 transfer reaction sequence
99 transfer reactions
100 transfer sequence
101 viridis
102 viridis photosynthetic reaction centers
103 vivo
104 whole cells
105 schema:name Time-resolved electron transfer at the donor side of Rhodopseudomonas viridis photosynthetic reaction centers in whole cells
106 schema:pagination 317-323
107 schema:productId N7e1336c42f76401d8a1278a53d7b1312
108 Nad3350b1148e46b495256f8d571cfcdd
109 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021972178
110 https://doi.org/10.1023/a:1005930018775
111 schema:sdDatePublished 2022-01-01T18:07
112 schema:sdLicense https://scigraph.springernature.com/explorer/license/
113 schema:sdPublisher N24c04f09b7b44d00aa216444ea176ae6
114 schema:url https://doi.org/10.1023/a:1005930018775
115 sgo:license sg:explorer/license/
116 sgo:sdDataset articles
117 rdf:type schema:ScholarlyArticle
118 N05c7731510c54ad79a35c04fa14d5154 schema:issueNumber 2-3
119 rdf:type schema:PublicationIssue
120 N24c04f09b7b44d00aa216444ea176ae6 schema:name Springer Nature - SN SciGraph project
121 rdf:type schema:Organization
122 N34912841757a4f8fb1a248b93e3743a4 rdf:first sg:person.0636622234.64
123 rdf:rest N73b8c6bbddd04ec49d9f86892649b80a
124 N6e4313609b5e419fa5e48249840de978 schema:volumeNumber 55
125 rdf:type schema:PublicationVolume
126 N706227826e3f4d128c5e9e6bd49f3ad7 rdf:first sg:person.01324041054.45
127 rdf:rest rdf:nil
128 N73b8c6bbddd04ec49d9f86892649b80a rdf:first sg:person.01207002310.01
129 rdf:rest N706227826e3f4d128c5e9e6bd49f3ad7
130 N7e1336c42f76401d8a1278a53d7b1312 schema:name dimensions_id
131 schema:value pub.1021972178
132 rdf:type schema:PropertyValue
133 Nad3350b1148e46b495256f8d571cfcdd schema:name doi
134 schema:value 10.1023/a:1005930018775
135 rdf:type schema:PropertyValue
136 Nd13a9e93cdf74847a7ddcbd2b8a0556c rdf:first sg:person.01103560265.56
137 rdf:rest N34912841757a4f8fb1a248b93e3743a4
138 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
139 schema:name Biological Sciences
140 rdf:type schema:DefinedTerm
141 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
142 schema:name Biochemistry and Cell Biology
143 rdf:type schema:DefinedTerm
144 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
145 schema:name Genetics
146 rdf:type schema:DefinedTerm
147 anzsrc-for:0607 schema:inDefinedTermSet anzsrc-for:
148 schema:name Plant Biology
149 rdf:type schema:DefinedTerm
150 sg:journal.1022986 schema:issn 0166-8595
151 1573-5079
152 schema:name Photosynthesis Research
153 schema:publisher Springer Nature
154 rdf:type schema:Periodical
155 sg:person.01103560265.56 schema:affiliation grid-institutes:grid.450875.b
156 schema:familyName Rappaport
157 schema:givenName Fabrice
158 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01103560265.56
159 rdf:type schema:Person
160 sg:person.01207002310.01 schema:affiliation grid-institutes:grid.457335.3
161 schema:familyName Verméglio
162 schema:givenName André
163 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01207002310.01
164 rdf:type schema:Person
165 sg:person.01324041054.45 schema:affiliation grid-institutes:grid.450875.b
166 schema:familyName Joliot
167 schema:givenName Pierre
168 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01324041054.45
169 rdf:type schema:Person
170 sg:person.0636622234.64 schema:affiliation grid-institutes:grid.450875.b
171 schema:familyName Béal
172 schema:givenName Daniel
173 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0636622234.64
174 rdf:type schema:Person
175 sg:pub.10.1007/bf00047946 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045641142
176 https://doi.org/10.1007/bf00047946
177 rdf:type schema:CreativeWork
178 sg:pub.10.1038/318618a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020599081
179 https://doi.org/10.1038/318618a0
180 rdf:type schema:CreativeWork
181 sg:pub.10.1038/355796a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032183790
182 https://doi.org/10.1038/355796a0
183 rdf:type schema:CreativeWork
184 grid-institutes:grid.450875.b schema:alternateName Institut de Biologie Physico-Chimique, CNRS UPR, 9072, 13 rue Pierre et Marie Curie, 75005, PARIS, France
185 schema:name Institut de Biologie Physico-Chimique, CNRS UPR, 9072, 13 rue Pierre et Marie Curie, 75005, PARIS, France
186 rdf:type schema:Organization
187 grid-institutes:grid.457335.3 schema:alternateName CEA Cadarache, DEVM/Laboratoire de Bioénergétique Cellulaire, 13108, St Paul-lez-Durance, France
188 schema:name CEA Cadarache, DEVM/Laboratoire de Bioénergétique Cellulaire, 13108, St Paul-lez-Durance, France
189 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...