PCR-identification of a Nicotiana plumbaginifolia cDNA homologous to the high-affinity nitrate transporters of the crnA family View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1997-05

AUTHORS

Alberto Quesada, Anne Krapp, Laurence J. Trueman, Françoise Daniel-Vedele, Emilio Fernández, Brian G. Forde, Michel Caboche

ABSTRACT

A family of high-affinity nitrate transporters has been identified in Aspergillus nidulans and Chlamydomonas reinhardtii, and recently homologues of this family have been cloned from a higher plant (barley). Based on six of the peptide sequences most strongly conserved between the barley and C. reinhardtii polypeptides, a set of degenerate primers was designed to permit amplification of the corresponding genes from other plant species. The utility of these primers was demonstrated by RT-PCR with cDNA made from poly(A)+ RNA from barley, C. reinhardtii and Nicotiana plumbaginifolia. A PCR fragment amplified from N. plumbaginifolia was used as probe to isolate a full-length cDNA clone which encodes a protein, NRT2;1Np, that is closely related to the previously isolated crnA homologue from barley. Genomic Southern blots indicated that there are only 1 or 2 members of the Nrt2 gene family in N. plumbaginifolia. Northern blotting showed that the Nrt2 transcripts are most strongly expressed in roots. The effects of external treatments with different N sources showed that the regulation of the Nrt2 gene(s) is very similar to that reported for nitrate reductase and nitrite reductase genes: their expression was strongly induced by nitrate but was repressed when reduced forms of N were supplied to the roots. More... »

PAGES

265-274

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1005872816881

DOI

http://dx.doi.org/10.1023/a:1005872816881

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1027045058

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/9207842


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RDF/XML is a standard XML format for linked data.

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