Ontology type: schema:ScholarlyArticle
1997-06
AUTHORSNataša Formanová, Gregory G. Brown
ABSTRACTRNA b is the most abundant member of a family of autonomously replicating single- and double-stranded RNA plasmids found in maize mitochondria. The extent to which this molecule is associated with proteins was investigated by rate zonal and CsCl equilibrium density gradient centrifugation of clarified lysates of S cytoplasm maize mitochondria. A soluble complex of RNA b, responsible for synthesis of the more abundant (+) RNA b strand in mitochondrial lysates, was identified. The complex had a buoyant density of 1.49 g/cm3, indicating a substantial non-nucleic acids content. The sedimentation coefficient of the complex, however, was only slightly larger than that of deproteinized RNA b. Synthesis of RNA b as well as the larger RNA plasmid, RNA a, was resistant to heparin, suggesting that, for both RNAs, preformed complexes between an RNA template and an RNA-dependent RNA polymerase capable of elongating in vivo preinitiated RNA plasmid strands, were present in the lysate. Only a small fraction of RNA b molecules were bound in the complex; the bulk of RNA b sedimented at the same rate as the deproteinized RNA. Thus, after replication, maize mitochondrial plasmids are not associated with nucleoprotein capsids although their synthesis takes place through ribonucleoprotein replication complexes. More... »
PAGES383-392
http://scigraph.springernature.com/pub.10.1023/a:1005818631401
DOIhttp://dx.doi.org/10.1023/a:1005818631401
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1043345943
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/9225850
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biochemistry and Cell Biology",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Centrifugation, Zonal",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mitochondria",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Plasmids",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "RNA Probes",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "RNA, Double-Stranded",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "RNA, Plant",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Ribonuclease, Pancreatic",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Zea mays",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Department of Biology, McGill University, 1205 Doctor Penfield Avenue, H3A 1B1, Montreal, Quebec, Canada",
"id": "http://www.grid.ac/institutes/grid.14709.3b",
"name": [
"Department of Biology, McGill University, 1205 Doctor Penfield Avenue, H3A 1B1, Montreal, Quebec, Canada"
],
"type": "Organization"
},
"familyName": "Formanov\u00e1",
"givenName": "Nata\u0161a",
"id": "sg:person.0675751306.25",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0675751306.25"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Biology, McGill University, 1205 Doctor Penfield Avenue, H3A 1B1, Montreal, Quebec, Canada",
"id": "http://www.grid.ac/institutes/grid.14709.3b",
"name": [
"Department of Biology, McGill University, 1205 Doctor Penfield Avenue, H3A 1B1, Montreal, Quebec, Canada"
],
"type": "Organization"
},
"familyName": "Brown",
"givenName": "Gregory G.",
"id": "sg:person.01041003047.82",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01041003047.82"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1007/bf00027494",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009354516",
"https://doi.org/10.1007/bf00027494"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/978-94-011-0163-9_2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1089529274",
"https://doi.org/10.1007/978-94-011-0163-9_2"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00199965",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022535090",
"https://doi.org/10.1007/bf00199965"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00334523",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1014627276",
"https://doi.org/10.1007/bf00334523"
],
"type": "CreativeWork"
}
],
"datePublished": "1997-06",
"datePublishedReg": "1997-06-01",
"description": "RNA b is the most abundant member of a family of autonomously replicating single- and double-stranded RNA plasmids found in maize mitochondria. The extent to which this molecule is associated with proteins was investigated by rate zonal and CsCl equilibrium density gradient centrifugation of clarified lysates of S cytoplasm maize mitochondria. A soluble complex of RNA b, responsible for synthesis of the more abundant (+) RNA b strand in mitochondrial lysates, was identified. The complex had a buoyant density of 1.49 g/cm3, indicating a substantial non-nucleic acids content. The sedimentation coefficient of the complex, however, was only slightly larger than that of deproteinized RNA b. Synthesis of RNA b as well as the larger RNA plasmid, RNA a, was resistant to heparin, suggesting that, for both RNAs, preformed complexes between an RNA template and an RNA-dependent RNA polymerase capable of elongating in vivo preinitiated RNA plasmid strands, were present in the lysate. Only a small fraction of RNA b molecules were bound in the complex; the bulk of RNA b sedimented at the same rate as the deproteinized RNA. Thus, after replication, maize mitochondrial plasmids are not associated with nucleoprotein capsids although their synthesis takes place through ribonucleoprotein replication complexes.",
"genre": "article",
"id": "sg:pub.10.1023/a:1005818631401",
"inLanguage": "en",
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1101246",
"issn": [
"0167-4412",
"1573-5028"
],
"name": "Plant Molecular Biology",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "3",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "34"
}
],
"keywords": [
"RNA B",
"maize mitochondria",
"RNA-dependent RNA polymerase",
"RNA plasmid",
"CsCl equilibrium density gradient centrifugation",
"mitochondrial lysate",
"mitochondrial plasmids",
"RNA polymerase",
"replication complex",
"abundant members",
"equilibrium density gradient centrifugation",
"RNA A",
"RNA template",
"plasmid strand",
"density gradient centrifugation",
"plasmid",
"lysates",
"mitochondria",
"buoyant density",
"RNA",
"gradient centrifugation",
"protein",
"sedimentation coefficient",
"complexes",
"B molecules",
"strands",
"acid content",
"polymerase",
"soluble complexes",
"capsid",
"small fraction",
"molecules",
"replication",
"same rate",
"synthesis",
"family",
"vivo",
"rate zonal",
"members",
"centrifugation",
"template",
"B.",
"content",
"fraction",
"extent",
"heparin",
"density",
"rate",
"bulk",
"place",
"coefficient",
"zonal",
"cm3"
],
"name": "The maize mitochondrial plasmid RNA b is associated with protein during synthesis but is not encapsidated",
"pagination": "383-392",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1043345943"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1023/a:1005818631401"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"9225850"
]
}
],
"sameAs": [
"https://doi.org/10.1023/a:1005818631401",
"https://app.dimensions.ai/details/publication/pub.1043345943"
],
"sdDataset": "articles",
"sdDatePublished": "2022-05-20T07:19",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220519/entities/gbq_results/article/article_264.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1023/a:1005818631401"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1023/a:1005818631401'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1023/a:1005818631401'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1023/a:1005818631401'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1023/a:1005818631401'
This table displays all metadata directly associated to this object as RDF triples.
170 TRIPLES
22 PREDICATES
92 URIs
80 LITERALS
15 BLANK NODES