Aspartic proteinase genes in the Brassicaceae Arabidopsis thaliana and Brassica napus View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1997-01

AUTHORS

Kathleen D'Hondt, Sylvia Stack, Steve Gutteridge, Joel Vandekerckhove, Enno Krebbers, Susannah Gal

ABSTRACT

Active aspartic proteinase is isolated from Brassica napus seeds and the peptide sequence is used to generate primers for PCR. We present here cDNA and genomic clones for aspartic proteinases from the closely related Brassicaceae Arabidopsis thaliana and Brassica napus. The Arabidopsis cDNA represents a single gene, while Brassica has at least 4 genes. Like other plant aspartic proteases, the two Brassicaceae enzymes contain an extra protein domain of about 100 amino acids relative to the mammalian forms. The intron/exon arrangement in the Brassica genomic clone is significantly different from that in mammalian genes. As the proteinase is isolated from seeds, the same tissue where 2S albumins are processed, this implies expression of one of the aspartic proteinase genes there. More... »

PAGES

187-192

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1005794917200

DOI

http://dx.doi.org/10.1023/a:1005794917200

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1028613485

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/9037171


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52 schema:description Active aspartic proteinase is isolated from Brassica napus seeds and the peptide sequence is used to generate primers for PCR. We present here cDNA and genomic clones for aspartic proteinases from the closely related Brassicaceae Arabidopsis thaliana and Brassica napus. The Arabidopsis cDNA represents a single gene, while Brassica has at least 4 genes. Like other plant aspartic proteases, the two Brassicaceae enzymes contain an extra protein domain of about 100 amino acids relative to the mammalian forms. The intron/exon arrangement in the Brassica genomic clone is significantly different from that in mammalian genes. As the proteinase is isolated from seeds, the same tissue where 2S albumins are processed, this implies expression of one of the aspartic proteinase genes there.
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