Gene delivery for genetically engineered mucosal cells with enhanced function View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-06

AUTHORS

Naoki Nagatani, Masashige Shinkai, Yayoi Nagase, Hiroyuki Honda, Ken-ichiro Hata, Hirokazu Mizuno, Minoru Ueda, Takeshi Kobayashi

ABSTRACT

A non-viral transfection method for oral mucosal cells was investigated using a modified transfection method and five commercial transfection reagents. The CellFECTINTM gave the highest expression of a transfected gene. When the mucosal cells were transfected with 0.3 ng DNA/cell, the transfection efficiency was optimal, and the production of a reporter protein increased up to ten times higher than those with the other transfection reagents. More... »

PAGES

999-1002

References to SciGraph publications

Journal

TITLE

Biotechnology Letters

ISSUE

12

VOLUME

22

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1005693321390

DOI

http://dx.doi.org/10.1023/a:1005693321390

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1000218395


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