Anti-phytochelatin monoclonal antibody View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-09

AUTHORS

Hiroyuki Satofuka, Satoshi Amano, Toshiaki Fukui, Haruyuki Atomi, Masahiro Takagi, Tadayuki Imanaka

ABSTRACT

Phytochelatins (PCs, (γGlu-Cys)n-Gly, n = 2–11) are metal ions-binding peptides produced by plant, algae and fungi. Antibodies that recognize PCs were induced by the injection of Balb/c mice with a multiple antigen peptide consisting of PC6(MAP-PC6). One stable hybridoma producing a monoclonal antibody (mAb), designated as 4-9C, was established. The DNA sequences of the heavy and light chain variable regions of the 4-9C mAb were determined. The 4-9C mAb had a smaller equilibrium dissociation constant (Kd) towards Cu-, Zn- and Ni-PC7complexes than those towards other metal-PC7complexes and free PCs. More... »

PAGES

1423-1428

Journal

TITLE

Biotechnology Letters

ISSUE

17

VOLUME

22

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1005673218300

DOI

http://dx.doi.org/10.1023/a:1005673218300

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1007874101


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