A thermostable glucose isomerase having a relatively low optimum pH: study of activity and molecular cloning of the corresponding gene View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-06

AUTHORS

Karima Srih-Belghith, Samir Bejar

ABSTRACT

A thermostable glucose isomerase from a newly isolated thermophilic Streptomyces sp. SK strain, had a wide pH range with an optimum of 6 at 60°C and 6.4 at 90°C. It was optimally active at 95°C and completely stable at 80°C for at least 5.5 h with a half-life of 5 h at 90°C. Using E.coli as a host strain and an internal fragment of xylA of S. olivochromogenes as a probe, a 6.5 kb DNA fragment from Streptomyces sp. SK was cloned. This fragment carries the entire xylA gene since the recombinant plasmid complements the E.coli xyl-5 mutant strain HB101. © Rapid Science Ltd. 1998 More... »

PAGES

553-556

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1005393510435

DOI

http://dx.doi.org/10.1023/a:1005393510435

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1041937453


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