A comparative assessment of DNA fingerprinting techniques (RAPD, ISSR, AFLP and SSR) in tetraploid potato (Solanum tuberosum L.) germplasm View Full Text


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Article Info

DATE

2000-05

AUTHORS

C.E. McGregor, C.A. Lambert, M.M. Greyling, J.H. Louw, L. Warnich

ABSTRACT

Several DNA marker systems and associated techniques are available today for fingerprinting plant germplasm but information on their relative usefulness in particular crops is limited. The study investigated PCR based DNA fingerprinting in a set of 39 potato cultivars using RAPDs (20 primers), ISSRs (6 primers), AFLPs (2 primers) and SSRs (5 primer pairs). Results show that each of the four techniques can on their own, individually identify each cultivar, but that techniques differ in the mean number of profiles generated per primer (or primer pair) per cultivar, referred to as Genotype Index (GI). The order of merit based on this criterium and in this material was AFLPs (GI = 1.0), a multi-locus SSR (GI = 0.77),RAPDs (GI = 0.53), ISSRs (GI = 0.47) and single locus SSRs (GI = 0.36). Problems in relating banding patterns to individual loci and alleles for polyploid genomes, using these techniques as they are currently employed, are also discussed. More... »

PAGES

135-144

References to SciGraph publications

  • 1994-08. Genetic relationships of Japanese potato cultivars assessed by RAPD analysis in AMERICAN JOURNAL OF POTATO RESEARCH
  • 1995-02. Application of two microsatellite sequences in wheat storage proteins as molecular markers in THEORETICAL AND APPLIED GENETICS
  • 1997-10. Identification of polymorphic DNA markers in cultivated peanut (Arachis hypogaea L.) in EUPHYTICA
  • 1993-10. Comparison of allozyme, RFLP, and RAPD markers for revealing genetic variation within and between trembling aspen and bigtooth aspen in THEORETICAL AND APPLIED GENETICS
  • 1995-12. Assessment of genetic diversity in dent and popcorn (Zea mays L.) inbred lines using inter-simple sequence repeat (ISSR) amplification in MOLECULAR BREEDING
  • 1997-10. Reproducibility testing of RAPD, AFLP and SSR markers in plants by a network of European laboratories in MOLECULAR BREEDING
  • 1998. Amplified Fragment Length Polymorphism (AFLP) in MOLECULAR TOOLS FOR SCREENING BIODIVERSITY
  • 1997-04. Comparison of PCR-based marker systems for the analysis of genetic relationships in cultivated potato in MOLECULAR BREEDING
  • 1998-05. Characterization of the genetic resources of redcurrant (Ribes rubrum: subg. Ribesia) using anchored microsatellite markers in THEORETICAL AND APPLIED GENETICS
  • 1996-06. Identification of molecular markers in soybean comparing RFLP, RAPD and AFLP DNA mapping techniques in PLANT MOLECULAR BIOLOGY REPORTER
  • 1996-08. Characterization ofSolanum tuberosum simple sequence repeats and application to potato culiwar identification in AMERICAN JOURNAL OF POTATO RESEARCH
  • 1996-12. Competition as a source of errors in RAPD analysis in THEORETICAL AND APPLIED GENETICS
  • 1996-01. Polymorphism and DNA markers for asparagus cultivars identified by random amplified polymorphic DNA in EUPHYTICA
  • 1996-05. Genetic diversity of potato determined by random amplified polymorphic DNA analysis in PLANT CELL REPORTS
  • 1995-02. Barley microsatellites: allele variation and mapping in PLANT MOLECULAR BIOLOGY
  • 1970-08. Isozyme polymorphisms in natural populations of Avena fatua and A. barbata in HEREDITY
  • 1996-09. The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis in MOLECULAR BREEDING
  • 1997-09. Direct comparison of levels of genetic variation among barley accessions detected by RFLPs, AFLPs, SSRs and RAPDs in THEORETICAL AND APPLIED GENETICS
  • 1994-01. Stability of RAPD markers for determining cultivar specific DNA profiles in rye (Secale cereale L.) in EUPHYTICA
  • 1997-04. Genome mapping, molecular markers and marker-assisted selection in crop plants in MOLECULAR BREEDING
  • Identifiers

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    DOI

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