Occasional loss of expression of phosphinothricin tolerance in sexual offspring of transgenic oilseed rape (Brassica napus L.) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1997-12

AUTHORS

Peter L.J. Metz, Evert Jacobsen, Willem J. Stiekema

ABSTRACT

The commercial and economic value of genetically modified crops is determined by a predictable, consistent and stable transmission and expression of the transgenes in successive generations. No gene inactivation is expected after selfings or crosses with non-transformed plants of homozygous transgenic oilseed rape plants if the expression of the transgene in homozygous or hemizygous nature in such plants is stable. The segregation ratios of phosphinothricin (PPT) tolerance in successive generations of selfings and mutual crosses of a few independent transgenic PPT-tolerant oilseed rape plants indicated a dominant, monogenic inheritance. In within-variety and between-variety crosses no transgene inactivation was observed. However, after selfings and backcrosses with non-transgenic oilseed rape infrequent loss of the expression of the PPT tolerance transgene was observed independent from its homozygous or hemizygous nature. Molecular analysis of PPT-susceptible plants showed that the loss of expression was due to gene inactivation and not to the absence of the transgene. Methylation and co-suppression are mechanisms that might cause reduced or even loss of expression of the transgene in later generations. The implications of this observation for seed multiplication of varieties and breeding activities with transgenic oilseed rape are discussed. More... »

PAGES

189-196

References to SciGraph publications

  • 1991-08. Reversible inactivation of a transgene in Arabidopsis thaliana in MOLECULAR GENETICS AND GENOMICS
  • 1995-06. Hybridization of radish (Raphanus sativus L.) and oilseed rape (Brassica napus L.) through a flower-culture method in EUPHYTICA
  • 1994-09. Transgene Inactivation: Plants Fight Back! in NATURE BIOTECHNOLOGY
  • 1993-11. Expression instability and genetic disorders in transgenicNicotiana plumbaginifolia L. plants in TRANSGENIC RESEARCH
  • 1995-02. Variation of transgene expression in plants in EUPHYTICA
  • 1995-02. Transgenic apples display stable gene expression in the fruit and Mendelian segregation of the transgenes in the R1 progeny in EUPHYTICA
  • 1990-07. Epigenetic changes in the expression of the maize A1 gene inPetunia hybrida: Role of numbers of integrated gene copies and state of methylation in MOLECULAR GENETICS AND GENOMICS
  • 1988-12. Genetic analysis of T-DNA insertions into the tobacco genome in PLANT CELL REPORTS
  • 1993-01. A variety of epistatic interactions can occur between partially homologous transgene loci brought together by sexual crossing in MOLECULAR GENETICS AND GENOMICS
  • 1987-04. High meiotic stability of a foreign gene introduced into tobacco by Agrobacterium-mediated transformation in MOLECULAR GENETICS AND GENOMICS
  • 1991-05. Differential inactivation and methylation of a transgene in plants by two suppressor loci containing homologous sequences in PLANT MOLECULAR BIOLOGY
  • 1995-11. Effect of oilseed rape genotype on the spontaneous hybridization rate with a weedy species:an assessment of transgene dispersal in THEORETICAL AND APPLIED GENETICS
  • 1995-02. The release of transgenic plants from containment, and the move towards their widespread use in agriculture in EUPHYTICA
  • 1992-10. Promoter methylation and progressive transgene inactivation inArabidopsis in PLANT MOLECULAR BIOLOGY
  • 1992-02. Endogenous and environmental factors influence 35S promoter methylation of a maize A1 gene construct in transgenic petunia and its colour phenotype in MOLECULAR GENETICS AND GENOMICS
  • 1988-05. Analysis of the T-DNA structure in a large number of transgenic petunias generated by Agrobacterium-mediated transformation in PLANT MOLECULAR BIOLOGY
  • 1988-05. Expression and inheritance of kanamycin resistance in a large number of transgenic petunias generated by Agrobacterium-mediated transformation in PLANT MOLECULAR BIOLOGY
  • 1986-09. The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vector in MOLECULAR GENETICS AND GENOMICS
  • Journal

    TITLE

    Euphytica

    ISSUE

    3

    VOLUME

    98

    Author Affiliations

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1023/a:1003117200129

    DOI

    http://dx.doi.org/10.1023/a:1003117200129

    DIMENSIONS

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    34 schema:description The commercial and economic value of genetically modified crops is determined by a predictable, consistent and stable transmission and expression of the transgenes in successive generations. No gene inactivation is expected after selfings or crosses with non-transformed plants of homozygous transgenic oilseed rape plants if the expression of the transgene in homozygous or hemizygous nature in such plants is stable. The segregation ratios of phosphinothricin (PPT) tolerance in successive generations of selfings and mutual crosses of a few independent transgenic PPT-tolerant oilseed rape plants indicated a dominant, monogenic inheritance. In within-variety and between-variety crosses no transgene inactivation was observed. However, after selfings and backcrosses with non-transgenic oilseed rape infrequent loss of the expression of the PPT tolerance transgene was observed independent from its homozygous or hemizygous nature. Molecular analysis of PPT-susceptible plants showed that the loss of expression was due to gene inactivation and not to the absence of the transgene. Methylation and co-suppression are mechanisms that might cause reduced or even loss of expression of the transgene in later generations. The implications of this observation for seed multiplication of varieties and breeding activities with transgenic oilseed rape are discussed.
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