Effect of Agar, MS Medium Strength, Sucrose and Polyamines on in vitro Rooting of Syzygium Alternifolium View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1999-11

AUTHORS

P.S. Sha Valli Khan, J.F. Hausman, K.R. Rao

ABSTRACT

This paper describes the effect of agar, MS basal medium strength, sucrose and polyamines on the in vitro rooting of Syzygium alternifolium realized by a two step procedure involving root initiation (RI) and root elongation (RE). RI was carried out on solidified MS medium supplemented with 1.0 mg dm−3 indole-3-butyric acid (IBA) for 3 weeks, and RE following transfer to half-strength MS medium devoid of growth regulators for another 3 weeks. Agar and MS basal medium concentrations played important role on rooting response as well as on health of rooted shoots. Sucrose concentration was positively correlated with the rooting percentage, root number per shoot and root length. The combination of polyamines and 1.0 mg dm−3 IBA increases rooting percentage compared to media containing only 1.0 mg dm−3 IBA. Optimum rooting was attained with half-strength MS medium containing 1.0 mg dm−3 IBA, 2 % sucrose, 10 µM spermine and 0.8 % agar. More... »

PAGES

333-340

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1023/a:1002400915965

DOI

http://dx.doi.org/10.1023/a:1002400915965

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1012182841


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